Granted Patent B2

US 12,409,648 · App. 18/531,501 · Granted Sep 9, 2025

Inventors: Sammy S. Datwani (Pleasanton, CA); Carson Riche (Fremont, CA); Marsha N. Blauwkamp (Palo Alto, CA); Justin Blonigan (Pleasanton, CA)

Assignee: LABCYTE INC.

B41J2/04575B01J19/0046B01J19/26B01L3/0268B41J2/04516B41J2/04588B01J2219/00362B01L2200/0647B01L2200/148B01L2400/0436B01L2400/0439

Abstract

Claims (39)

1. A method of ejecting a droplet from a reservoir containing a non-Newtonian fluid by an acoustic droplet ejector, the method comprising:

emitting focused acoustic energy in a first pattern comprising a first tone burst segment to a fluid surface of the non-Newtonian fluid to generate a leading lobe and a filament from the fluid surface without separating the filament from the leading lobe or the fluid surface, wherein a diameter of the leading lobe is greater than a diameter of the filament; and

emitting focused acoustic energy in a second pattern comprising a second tone burst segment and a third tone burst segment to the leading lobe to eject a droplet by separating the leading lobe from the filament to form the droplet, wherein an amplitude of the focused acoustic energy varies between the first tone burst segment and the second tone burst segment or the second tone burst segment and the third tone burst segment, wherein each of the first tone burst segment and the third tone burst segment include a plurality of cycles of waveform.

2. The method of claim 1 , wherein:

the first tone burst segment has an amplitude and duration sufficient to raise the leading lobe and filament from the fluid surface; and

the second pattern comprises at least two discrete tone burst segments including the second tone burst segment and the third tone burst segment configured to eject the droplet by separating the droplet from the filament.

3. The method of claim 2 , wherein the first tone burst segment has greater duration than each one of the second and third tone burst segments, and third tone burst segment having greater duration than the second tone burst segment.

4. The method of claim 1 , wherein the droplet has a diameter less than 70%, less than 30%, or less than 10% of a length of the filament, or wherein the droplet has a droplet volume less than 80%, less than 10%, or less than 5% of a total volume of a remainder of the filament.

5. The method of claim 1 , wherein the second pattern of focused acoustic energy is configured to effect droplet ejection without producing satellite droplets.

6. The method of claim 1 , wherein an amplitude of the focused acoustic energy exceeds an alternative amplitude associated with ejecting an alternative droplet from a Newtonian fluid.

7. The method of claim 1 , wherein the non-Newtonian fluid comprises a mixture of water and genomic DNA.

8. The method of claim 1 , wherein:

the filament of the non-Newtonian fluid generated from the reservoir by the focused acoustic energy comprises a chain containing 0, 1, 2, or >2 beads of the non-Newtonian fluid; and

the second pattern of focused acoustic energy induces droplet breakoff from the chain.

9. The method of claim 8 , wherein the beads on the chain coalesce with each other or with the non-Newtonian fluid in the reservoir before or after droplet breakoff.

10. The method of claim 8 , further comprising optically or electronically monitoring the filament to assess whether the droplet breakoff is successful or unsuccessful.

11. The method of claim 1 , further comprising predicting droplet breakoff based on optical or electrical signals from a fluid mound formed in the reservoir.

12. The method of claim 1 , wherein the non-Newtonian fluid comprises a solution containing genetic material.

13. The method of claim 1 , further comprising:

emitting a series of calibration tone bursts to the fluid surface configured to raise a quantity of fluid from the fluid surface;

detecting formation of a filament in the quantity of fluid raised from the fluid surface or detecting ejection of a calibration droplet from the fluid surface; and

setting one of a power, wavelength, or duration of one or more tone bursts of the first pattern of focused acoustic energy or the second pattern of focused acoustic energy based on the detection of the filament or the ejection of the calibration droplet.

14. A droplet ejection system configured to eject a droplet from a non-Newtonian fluid in a reservoir, the system comprising:

an acoustic ejector configured to:

emit focused acoustic energy in a first pattern comprising a first tone burst segment to a fluid surface of the non-Newtonian fluid to generate a leading lobe and a filament from the fluid surface without separating the filament from the leading lobe or the fluid surface, wherein a diameter of the leading lobe is greater than a diameter of the filament; and

emit focused acoustic energy in a second pattern comprising a second tone burst segment and a third tone burst segment to the leading lobe to separate the leading lobe from the filament without ejecting satellite droplets such that the filament retreats into the fluid surface, wherein an amplitude of the focused acoustic energy varies between the first tone burst segment and the second tone burst segment or the second tone burst segment and the third tone burst segment; and

a processor and memory device storing executable instructions that, when executed by the processor, cause the acoustic ejector to emit the focused acoustic energy in the first pattern to a fluid surface of the non-Newtonian fluid and emit focused acoustic energy in the second pattern to the leading lobe, wherein each of the first tone burst segment and the third tone burst segment include a plurality of cycles of waveform.

15. The system of claim 14 , wherein the non-Newtonian fluid comprises a mixture of water and genomic DNA.

16. A method of ejecting a droplet containing a non-Newtonian fluid from a fluid reservoir, the method comprising:

emitting a tone burst pattern of focused acoustic energy to a fluid reservoir containing a non-Newtonian fluid, wherein the emission of the tone burst pattern to the fluid reservoir ejects a droplet of the non-Newtonian fluid from the fluid reservoir, and wherein the tone burst pattern comprises:

a first discrete tone burst segment configured to generate a leading lobe and a filament from a fluid surface of the fluid reservoir without separating the filament from the leading lobe or the fluid surface, wherein a diameter of the leading lobe is greater than a diameter of the filament;

a second discrete tone burst segment emitted subsequent to the first discrete tone burst segment; and

a third discrete tone burst segment emitted subsequent to the second discrete tone burst segment, wherein an amplitude of the focused acoustic energy varies between the first discrete tone burst segment and the second discrete tone burst segment or the second discrete tone burst segment and the third discrete tone burst segment, wherein each of the first discrete tone burst segment and the third discrete tone burst segment include a plurality of cycles of waveform.

17. The method of claim 16 , wherein the first discrete tone burst segment has a greater duration than the second discrete tone burst segment and the third discrete tone burst segment, and the third discrete tone burst segment has a greater duration than the second discrete tone burst segment.

18. The method of claim 16 , wherein the non-Newtonian fluid comprises a mixture of water and genomic DNA.

19. The method of claim 16 , further comprising:

emitting a series of calibration tone bursts to the fluid surface configured to raise a quantity of fluid from the fluid surface;

detecting formation of the filament in the quantity of fluid raised from the fluid surface; and

setting one of a power, wavelength, or duration of one or more tone burst segments of the tone burst pattern based on the detection of the filament.

Assignments (1)

Continuity (3)

References Cited (100)