Process of extracting oil from thin stillage
A process of recovering oil, comprising (a) converting a starch-containing material into dextrins with an alpha-amylase; (b) saccharifying the dextrins using a carbohydrate source generating enzyme to form a sugar; (c) fermenting the sugar in a fermentation medium into a fermentation product using a fermenting organism; (d) recovering the fermentation product to form a whole stillage; (e) separating the whole stillage into thin stillage and wet cake; (e′) optionally concentrating the thin stillage into syrup; (f) recovering oil from the thin stillage and/or optionally the syrup, wherein a phospholipase is present and/or added during steps (a) to (c). Use of phospholipase for increasing oil recovery yields from thin stillage and/or syrup in a fermentation product production process.
1. A process of recovering oil, comprising:
(a) converting a starch-containing material into dextrins with an alpha-amylase at a temperature above the initial gelatinization temperature;
(b) saccharifying the dextrins using a carbohydrate source generating enzyme to form a sugar;
(c) fermenting the sugar in a fermentation medium into a fermentation product using a fermenting organism;
(d) recovering the fermentation product to form a whole stillage;
(e) separating the whole stillage into thin stillage and wet cake;
(f) optionally concentrating the thin stillage into syrup; and
(g) recovering oil from the thin stillage and/or optionally the syrup,
wherein a phospholipase C having at least 80% sequence identity to the amino acid sequence of SEQ ID NO: 2 is present and/or added during steps (b) and/or (c).
2. The process of claim 1 , wherein the phospholipase C is added during step (b).
3. The process of claim 1 , wherein the phospholipase C is added during step (c).
4. The process of claim 1 , wherein the saccharifying step (b) and fermenting step (c) are performed as a simultaneous saccharification and fermentation (SSF) and the phospholipase C is added during SSF.
5. The process of claim 1 , wherein a surfactant is present and/or added during steps (b) and/or (c).
6. The process of claim 5 , wherein the surfactant is selected from the group consisting of: polyethylene glycol, sorbitan monooleate, sorbitane monooleate, and mixtures thereof.
7. The process of claim 1 , wherein the phospholipase C has at least 90% sequence identity to the amino acid sequence of SEQ ID NO: 2.
8. The process of claim 1 , wherein the phospholipase C has at least 95% sequence identity to the amino acid sequence of SEQ ID NO: 2.
9. A process of recovering oil, comprising:
(a) converting a starch-containing material into dextrins with an alpha-amylase at a temperature below the initial gelatinization temperature;
(b) saccharifying the dextrins using a carbohydrate source generating enzyme to form a sugar;
(c) fermenting the sugar in a fermentation medium into a fermentation product using a fermenting organism;
(d) recovering the fermentation product to form a whole stillage;
(e) separating the whole stillage into thin stillage and wet cake;
(f) optionally concentrating the thin stillage into syrup; and
(g) recovering oil from the thin stillage and/or optionally the syrup,
wherein a phospholipase C having at least 80% sequence identity to the amino acid sequence of SEQ ID NO: 2 is present and/or added during steps (a), (b) and/or (c).
10. The process of claim 9 , wherein steps (b) and (c) are carried out simultaneously.
11. The process of claim 10 , wherein the phospholipase C is added during step (a).
12. The process of claim 11 , wherein a surfactant is present and/or added during step (a).
13. The process of claim 10 , wherein the phospholipase C is added during step (b) and/or step (c).
14. The process of claim 13 , wherein a surfactant is present and/or added during step (b) and/or step (c).
15. The process of claim 9 , wherein steps (a) to (c) are carried out simultaneously and the phospholipase is added during steps (a) to (c).
16. The process of claim 15 , wherein a surfactant is present and/or added during steps (a), (b) and/or (c).
17. The process of claim 9 , wherein the phospholipase C has at least 90% sequence identity to the amino acid sequence of SEQ ID NO: 2.
18. The process of claim 9 , wherein the phospholipase C has at least 95% sequence identity to the amino acid sequence of SEQ ID NO: 2.
19. The process of claim 9 , wherein the phospholipase C comprises the amino acid sequence of SEQ ID NO: 2.
20. The process of claim 1 , wherein the phospholipase C comprises the amino acid sequence of SEQ ID NO: 2.