Yeast expressing a heterologous phospholipase for ethanol production
Described herein are recombinant fermenting organisms having a heterologous polynucleotide encoding a phospholipase. Also described are processes for producing a fermentation product, such as ethanol, from starch or cellulosic-containing material with the recombinant fermenting organisms.
1. A recombinant Saccharomyces yeast cell comprising a heterologous polynucleotide encoding a phospholipase and a heterologous polynucleotide encoding a glucoamylase,
wherein the phospholipase has a mature polypeptide sequence at least 90% sequence identity to the amino acid sequence of SEQ ID NO: 237.
2. The recombinant yeast cell of claim 1 , wherein the fermenting organism comprises a heterologous polynucleotide encoding an alpha-amylase.
3. The recombinant yeast cell of claim 1 , wherein the fermenting organism comprises a heterologous polynucleotide encoding a protease.
4. The recombinant yeast of claim 1 , wherein the cell is a Saccharomyces cerevisiae cell.
5. The recombinant yeast of claim 1 , wherein the phospholipase has a mature polypeptide sequence having at least 95% sequence identity to the amino acid sequence of SEQ ID NO: 237.
6. The recombinant yeast of claim 1 , wherein the phospholipase has a mature polypeptide sequence having at least 97% sequence identity to the amino acid sequence of SEQ ID NO: 237.
7. The recombinant yeast of claim 1 , wherein the phospholipase has a mature polypeptide sequence having at least 98% sequence identity to the amino acid sequence of SEQ ID NO: 237.
8. The recombinant yeast of claim 1 , wherein the phospholipase has a mature polypeptide sequence having at least 99% sequence identity to the amino acid sequence of SEQ ID NO: 237.
9. The recombinant yeast of claim 1 , wherein the phospholipase has a mature polypeptide sequence that comprises or consists of the amino acid sequence of SEQ ID NO: 237.
10. A method of producing a fermentation product from a starch-containing or cellulosic-containing material comprising:
(a) saccharifying the starch-containing or cellulosic-containing material; and
(b) fermenting the saccharified material of step (a) with the recombinant yeast of claim 1 .
11. The method of claim 10 , wherein saccharification of step (a) occurs on a starch-containing material, and wherein the starch-containing material is gelatinized.
12. The method of claim 10 , wherein saccharification of step (a) occurs on a starch-containing material, and wherein the starch-containing material is ungelatinized.
13. The method of claim 10 , wherein saccharification of step (a) occurs on a starch-containing material, and wherein the method comprises liquefying the starch-containing material by contacting the material with an alpha-amylase prior to saccharification.
14. The method of claim 10 , wherein liquefying the starch-containing material and/or saccharifying the starch-containing material is conducted in presence of exogenously added protease.
15. The method of claim 10 , wherein the recombinant yeast comprises a heterologous polynucleotide encoding an alpha-amylase.
16. The method of claim 10 , wherein the recombinant yeast is a Saccharomyces cerevisiae cell.
17. The method of claim 10 , wherein the method results in higher yield of fermentation product and/or reduced foam accumulation when compared to the same process using an identical cell without the heterologous polynucleotide encoding the phospholipase under the same conditions.
18. The method of claim 10 , wherein the phospholipase of the recombinant yeast has a mature polypeptide sequence having at least 95% sequence identity to the amino acid sequence of SEQ ID NO: 237.
19. The method of claim 10 , wherein the phospholipase of the recombinant yeast has a mature polypeptide sequence having at least 97% sequence identity to the amino acid sequence of SEQ ID NO: 237.
20. The method of claim 10 , wherein the phospholipase of the recombinant yeast has a mature polypeptide sequence that comprises or consists of the amino acid sequence of SEQ ID NO: 237.