Granted Patent B2

US 12,410,451 · App. 18/516,805 · Granted Sep 9, 2025

Inventors: Michael Glenn Catlett (West Sacramento, CA); Monica Tassone (West Sacramento, CA); Paul Vincent Harris (Carnation, WA); Robert Lyle Osborne (Raleigh, NC); Shiro Fukuyama (Chiba, JP); Tomoko Matsui (Chiba, JP); Ryoko Kataoka (Longmont, CO)

Assignee: Novozymes A/S

C12P7/06C12P2203/00C12Y302/01001C12Y302/01093

Abstract

Claims (16)

1. A method of producing a fermentation product from a starch-containing or cellulosic-containing material comprising:

(a) saccharifying the starch-containing or cellulosic-containing material; and

(b) fermenting the saccharified material of step (a) with a recombinant yeast cell;

wherein the recombinant yeast cell comprises a heterologous polynucleotide encoding a trehalase having a mature polypeptide sequence with at least 95% sequence identity to the amino acid sequence of SEQ ID NO: 223.

2. The method of claim 1 , wherein the trehalase has a mature polypeptide sequence comprising or consisting of the amino acid sequence of SEQ ID NO: 223.

3. The method of claim 1 , wherein saccharification of step (a) occurs on a starch-containing material.

4. The method of claim 3 , comprising liquefying the starch-containing material by contacting the material with an alpha-amylase prior to saccharification.

5. The method of claim 4 , wherein liquefying the starch-containing material and/or saccharifying the starch-containing material is conducted in presence of exogenously added protease.

6. The method of claim 1 , wherein fermentation and saccharification are performed simultaneously in a simultaneous saccharification and fermentation (SSF).

7. The method of claim 1 , comprising recovering the fermentation product from the from the fermentation.

8. The method of claim 1 , wherein the fermentation product is ethanol.

9. The method of claim 1 , wherein the recombinant yeast cell comprises a heterologous polynucleotide encoding a glucoamylase or a protease.

10. The method of claim 1 , wherein the recombinant yeast cell is a Saccharomyces cerevisiae cell.

11. The method of claim 1 , wherein the trehalase has a mature polypeptide sequence with at least 97% sequence identity to the amino acid sequence of SEQ ID NO: 223.

12. The method of claim 1 , wherein the trehalase has a mature polypeptide sequence with at least 98% sequence identity to the amino acid sequence of SEQ ID NO: 223.

13. The method of claim 1 , wherein the trehalase has a mature polypeptide sequence with at least 99% sequence identity to the amino acid sequence of SEQ ID NO: 223.

Continuity (3)

References Cited (23)