Granted Patent B2

US 12,410,439 · App. 17/757,595 · Granted Sep 9, 2025

Inventors: Jeffrey Habben (Urbandale, IA); Benjamin P Weers (Polk City, IA); Jingrui Wu (Johnston, IA)

Assignee: PIONEER HI-BRED INTERNATIONAL, INC.

C12N15/8262A01H6/4684Y02A40/146

Abstract

Claims (18)

1. A corn plant comprising a stature reducing genetic modification and a yield enhancing genetic modification, wherein the yield enhancing genetic modification comprises a heterologous regulatory polynucleotide operably linked to a polynucleotide that encodes a polypeptide comprising an amino acid sequence that is at least 99% identical to SEQ ID NO: 1 and wherein the corn plant exhibits a plant height reduction of about 5% to about 50% due to the introduced genetic modification at a genomic locus encoding a D8 polypeptide represented by SEQ ID NO: 6, when compared to a control corn plant not comprising the stature reducing genetic modification.

2. The corn plant of claim 1 , wherein the plant comprises event DP-202216-6, wherein a representative sample of seed of said corn event has been deposited with American Type Culture Collection (ATCC) with Accession No. PTA-124653.

3. A seed produced from the corn plant of claim 1 , wherein a progeny from the seed exhibits increased yield and reduced stature when compared to the control plant.

4. A method of increasing planting density of corn plants comprising a heterologous regulatory polynucleotide operably linked to a polynucleotide that encodes a polypeptide comprising an amino acid sequence that is at least 99% identical to SEQ ID NO: 1, the method comprising:

a) providing corn plants wherein the expression of the polynucleotide encoding the polypeptide is increased when compared to a control plant;

b) reducing plant height by introducing a genetic modification at a genomic locus encoding a D8 polypeptide represented by SEQ ID NO: 6 that results in reduced stature of the corn plants; and

c) planting the corn plants at a planting density of about 30,000 to about 75,000 plants per acre.

5. The method of claim 4 , wherein the corn plants comprise event DP-202216-6.

6. The method of claim 4 , wherein the corn plants are planted in a plurality of rows having a row width of about 8 inches to about 30 inches.

7. The method of claim 4 , wherein the corn plants yield an average of about 3 bu/acre compared to control corn plants.

8. A method of increasing nitrogen use efficiency of a population of corn plants per unit of applied nitrogen, the method comprising:

a) providing the population of corn plants, wherein the expression of a polynucleotide that encodes a polypeptide comprising an amino acid sequence that is at least 99% identical to SEQ ID NO: 1 is increased due to a genetic modification compared to a control plant;

b) modifying the plant height of the population of the corn plants by introducing a genetic modification at a genomic locus encoding a D8 polypeptide represented by SEQ ID NO: 6 that results in reduced stature of the population of the corn plants; and

c) growing the population of plants in a crop growing environment where the applied nitrogen is about 10% to about 50% less than an application rate of about 100 lbs to about 400 lbs of nitrogen per acre.

9. The method of claim 8 , wherein the corn plants comprise event DP-202216-6.

10. The method of claim 8 , wherein the corn plants exhibit an increase in grain yield of an average of at least 3 bu/acre when compared to the control plant.

11. The method of claim 8 , wherein the population of corn plants exhibit an increase in nitrogen use efficiency of at least about 5% when compared to the control plant.

12. The method of claim 8 , wherein the nitrogen use efficiency is increased by applying late season nitrogen, wherein at least about 25% of total nitrogen applied is applied on or after V8-V12 stage.

Assignments (1)

Continuity (2)

References Cited (126)