Granted Patent B2

US 12,447,500 · App. 18/543,320 · Granted Oct 21, 2025

Inventors: Yannan Zhao (Del Mar, CA); Florika Macazo (San Diego, CA); Rachel Goodridge (San Diego, CA); Emily Welch (San Diego, CA); Hassan Bohra (Singapore, SG); Sang Park (San Diego, CA); Lisa Savagian (San Diego, CA); Borhan Chueh (San Diego, CA); Thomas Brubaker (Escondido, CA); Shreshtha Mishra (San Diego, CA)

Assignee: Illumina, Inc.

B05D1/38B05D7/24B05D7/22

Abstract

Claims (26)

1. A method for modifying an interstitial surface separating recesses from one another, the method comprising:

flowing a first fluid over the interstitial surface and into the recesses, such that the interstitial surface is substantially coated with the first fluid and the recesses are substantially filled with the first fluid;

while the first fluid remains within the recesses, replacing the first fluid coating the interstitial surface with a second fluid comprising a reagent; and

while the first fluid remains within the recesses and after replacing the first fluid coating the interstitial surface with the second fluid, solidifying the first fluid.

2. The method of claim 1 , further comprising using the reagent to modify the interstitial surface.

3. The method of claim 1 , wherein the first fluid remaining within the recesses substantially inhibits the reagent from modifying the recesses.

4. The method of claim 1 , wherein the first fluid and the second fluid are substantially immiscible with each other.

5. The method of claim 1 , wherein the first fluid is polar and the second fluid is nonpolar, or wherein the first fluid is nonpolar and the second fluid is polar.

6. The method of claim 1 , wherein the second fluid displaces the first fluid from the interstitial surface.

7. The method of claim 1 , wherein a third fluid displaces the first fluid from the interstitial surface before the second fluid replaces the first fluid coating the interstitial surface.

8. The method of claim 7 , wherein the third fluid is substantially immiscible with the second fluid.

9. The method of claim 7 , wherein the third fluid is miscible with the first fluid.

10. The method of claim 7 , wherein the second fluid displaces the third fluid from the interstitial surface.

11. The method of claim 1 , wherein the first fluid is solidified using a change in temperature, a change in pH, light, mechanical force, a reactive gas, an electrical field, a magnetic field, or a chemical.

12. The method of claim 1 , further comprising, after solidifying the first fluid within the recesses:

melting the solidified first fluid; and

removing the melted first fluid from the recesses.

13. The method of claim 1 , wherein the second fluid comprises a gas.

14. The method of claim 1 , wherein a cover is disposed over the interstitial surface and the recesses, forming a space between the cover and the interstitial surface.

15. The method of claim 14 , comprising using laminar flow to flow the first and second fluids in the space between the cover and the interstitial surface.

16. The method of claim 1 , wherein the first fluid and the second fluid both comprise liquids.

17. The method of claim 1 , wherein one of the first fluid and the second fluid is a liquid, and the other of the first fluid and the second fluid is a gas.

18. A method for modifying recesses that are separated from one another by an interstitial surface, the method comprising:

flowing a first fluid comprising a reagent over the interstitial surface and into the recesses, such that the interstitial surface is substantially coated with the first fluid and the recesses are substantially filled with the first fluid;

while the first fluid remains within the recesses, replacing the first fluid coating the interstitial surface with a second fluid;

wherein one of the first fluid and the second fluid is a liquid, and the other of the first fluid and the second fluid is a gas.

Assignments (3)

Continuity (2)

References Cited (9)