IP Library › Granted Patent US 12,448,423
Granted Patent B2
US 12,448,423 · App. 17/968,611 · Granted Oct 21, 2025

Targeted heterodimeric Fc fusion proteins containing NKG2D antigen binding domains

Inventors: Matthew J. Bernett (Monrovia, CA); John R. Desjarlais (Pasadena, CA); Rajat Varma (Monrovia, CA); Gregory Moore (Azusa, CA); Juan Diaz (Anaheim Hills, CA); Alex Nisthal (Monrovia, CA); Ke Liu (Glendora, CA); Rumana Rashid (Temple City, CA); Eric Chang (Walnut, CA)
Assignee: Xencor, Inc.
C07K14/5443A61P35/00C07K16/2851A61K38/00A61K2039/505C07K2317/622C07K2317/94C07K2319/30
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Quick Facts
Patent No.
US 12,448,423
App. No.
17/968,611
Granted
Oct 21, 2025
Kind
B2
Abstract

The present invention is directed to a novel targeted heterodimeric Fc fusion proteins comprising an IL-15/IL-15Rα Fc fusion protein and an NKG2D antigen binding domain Fc fusion proteins.

Claims (15)

1. A targeted molecule comprising:

an NKG2D antigen binding domain (ABD), said ABD comprising a variable heavy chain domain (“VH domain”) and a variable light chain domain (“VL domain”), wherein said VH domain has at least 95% amino acid sequence identity with SEQ ID NO: 1040 and said VL domain has at least 95% amino acid sequence identity with SEQ ID NO: 1041.

2. The targeted molecule of claim 1 , wherein said VH domain has at least 97% amino acid sequence identity with SEQ ID NO: 1040 and said VL domain has at least 97% amino acid sequence identity with SEQ ID NO: 1041.

3. The targeted molecule of claim 1 , wherein said VH domain has at least 98% amino acid sequence identity with SEQ ID NO: 1040 and said VL domain has at least 98% amino acid sequence identity with SEQ ID NO: 1041.

4. The targeted molecule of claim 1 , wherein said VH domain has at least 99% amino acid sequence identity with SEQ ID NO: 1040 and said VL domain has at least 99% amino acid sequence identity with SEQ ID NO: 1041.

5. The targeted molecule of claim 1 , wherein said VH domain is SEQ ID NO: 1040 and said VL domain is SEQ ID NO: 1041.

6. The targeted molecule of claim 1 , wherein said ABD is a single chain variable fragment (scFv) and further comprises a scFv linker that covalently attaches said VH domain and VL domain.

7. The targeted molecule of claim 6 , wherein said ABD from N- to C-terminus is oriented as VH-scFv linker-VL.

8. The targeted molecule of claim 6 , wherein said ABD from N- to C-terminus is oriented as VL-scFv linker-VH.

9. The targeted molecule of claim 1 , wherein said ABD is a Fab fragment.

10. A nucleic acid composition comprising one or more nucleic acid sequences encoding said ABD of claim 6 .

11. An expression vector composition comprising one or more expression vectors comprising said nucleic acid composition of claim 10 .

12. A host cell comprising said expression vector composition of claim 11 .

13. A method of producing a polypeptide comprising culturing said host cell of claim 12 under suitable conditions wherein said polypeptide is expressed, and recovering said polypeptide.

14. A pharmaceutical composition comprising the targeted molecule of claim 1 and a pharmaceutically acceptable carrier.

Assignments (1)
ASSIGNMENT OF ASSIGNOR'S INTEREST Recorded Nov 3, 2023
From: BERNETT, MATTHEW J.; DESJARLAIS, JOHN R.; VARMA, RAJAT; MOORE, GREGORY; DIAZ, JUAN; NISTHAL, ALEX; LIU, KE; RASHID, RUMANA; CHANG, ERIC
To: XENCOR, INC.
Reel/Frame 065455/0001 →
Continuity (3)
Division 16724118 · Dec 20, 2019
Provisional Application 62783106 · Dec 20, 2018
Related Publication 20230340053A1 · Oct 26, 2023
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