Transgenic Cory
View Patent ↗The present invention relates to transgenic ornamental fish, as well as methods of making such fish by germ cell transplantation techniques. Also disclosed are methods of establishing a population of such transgenic fish and methods of providing them to the ornamental fish industry for the purpose of marketing.
1 . A transgenic Cory whose genome comprises a chromosomally integrated expression cassette encoding a red fluorescent protein, wherein the transgenic Cory comprise the Red Cory 1 transformation event, germ stem cells comprising the Red Cory 1 transformation event having been deposited as ATCC accession number 127094, wherein the transgenic Cory exhibits red fluorescence.
2 . The transgenic Cory of claim 1 , wherein the transgenic cory is fertile.
3 . The transgenic Cory of claim 1 , wherein the transgenic Cory is homozygous for the integrated expression cassette.
4 . The transgenic Cory of claim 1 , wherein the transgenic Cory is heterozygous for the integrated expression cassette.
5 . A method of producing a transgenic Cory comprising:
(a) obtaining a Cory whose genome comprises a chromosomally integrated expression cassette encoding a red fluorescent protein, wherein the transgenic Cory comprise the Red Cory 1 transformation event, germ stem cells comprising the Red Cory 1 transformation event having been deposited as ATCC accession number 127094; and
breeding the obtained Cory with a second Cory to provide a transgenic Cory comprising the Red Cory 1 transformation event having been deposited as ATCC accession no. PTA-127094, wherein the transgenic Cory exhibits red fluorescence.
6 . The method of claim 5 , wherein the second Cory is a non-transgenic Cory.