Granted Patent B2

US 12,480,907 · App. 17/719,760 · Granted Nov 25, 2025

Inventors: Steven Koester (Edina, MN); Qun Su (Minneapolis, MN); Philippe Pierre Joseph Buhlmann (Minneapolis, MN); Xue Zhen (Plymouth Meeting, PA); Justin Theodore Nelson (Vadnais Heights, MN); Gregory J. Sherwood (White Bear Lake, MN)

Assignee: REGENTS OF THE UNIVERSITY OF MINNESOTA

G01N27/22G01N33/497H10D1/64H10D62/882G01N33/4975

Abstract

Claims (35)

1 . A system for analyzing a fluid sample comprising:

a controller circuit; and

a chemical sensor element, the chemical sensor element comprising

one or more discrete binding detectors, the one or more discrete binding detectors comprising one or more graphene varactors;

measurement circuitry, the measurement circuitry comprising

an electrical voltage generator, wherein the electrical voltage generator is configured to generate an applied voltage at a plurality of voltage values to be applied to the one or more graphene varactors, the voltage values falling within a range from a lower bound to an upper bound; and

a measurement circuit comprising a capacitance sensor, wherein the capacitance sensor is configured to measure capacitance of the discrete binding detectors resulting from the applied voltage;

wherein the system for analyzing the fluid sample is configured to measure hysteresis effects related to capacitance versus voltage values obtained from the one or more graphene varactors; and

wherein the system is configured to measure analyte presence in the fluid sample by evaluating hysteresis change in one or more parameters of capacitance-voltage data.

2 . The system of claim 1 , wherein the applied voltage comprises voltage values starting at one of the lower or upper bounds and moving to the other bound as part of a sweep across different voltage values falling within the range from the lower bound to the upper bound; and

wherein the hysteresis effects reflect differences in measurable values relating to capacitance of the graphene varactors resulting from a sweep in a first direction between the lower bound and upper bound versus a sweep in a second direction between the lower bound and the upper bound, wherein the second direction is opposite the first direction.

3 . The system of claim 2 , wherein a sweep in the first direction is followed by a pause and then sweep in the second direction.

4 . The system of claim 1 , wherein the applied voltage is represented as a sum of an AC voltage component superimposed on a DC bias voltage component.

5 . The system of claim 2 , wherein a sweep in the first direction followed by a sweep in the second direction constitutes a hysteresis measurement cycle, wherein the upper voltage bound and the lower voltage bound remain static between successive cycles.

6 . The system of claim 2 , wherein a sweep in the first direction followed by a sweep in the second direction constitutes a hysteresis measurement cycle, wherein the upper voltage bound and the lower voltage bound change between successive cycles.

7 . The system of claim 2 , wherein the system is configured to utilize determined hysteresis effects as a data input in a pattern matching operation, wherein results of the pattern matching operation characterizes the fluid test sample and/or a patient providing the fluid test sample.

8 . The system of claim 2 , wherein the system is configured to calculate a first Dirac point voltage for a sweep of the discrete binding detectors in the first direction and a second Dirac point voltage for a successive sweep of the discrete binding detectors in the second direction.

9 . The system of claim 1 , wherein the controller circuit is configured to calculate an average hysteresis change value of a measured property over a plurality of hysteresis measurement cycles.

10 . The system of claim 1 , wherein the system is configured to determine an identity of one or more analytes present in a fluid sample by evaluating hysteresis effects on one or more properties of the one or more discrete binding detectors.

11 . The system of claim 1 , further comprising a temperature controller configured to control a temperature of the one or more graphene varactors.

12 . A method for evaluating a fluid sample comprising:

contacting a chemical sensor element comprising one or more discrete binding detectors with the fluid sample, each discrete binding detector comprising a graphene varactor;

applying a voltage to the graphene varactors as part of a series of hysteresis measurement cycles over a time period, wherein each hysteresis measurement cycle includes applying the voltage to the graphene varactors as part of a sweep across a range of voltages in a first direction and then in a second direction that is opposite the first direction;

measuring capacitance of each of the discrete binding detectors resulting from the applied voltage;

determining hysteresis effects on measured capacitance values over the time period; and

determining an identity of one or more analytes present in the fluid sample by evaluating hysteresis effects on one or more properties of the one or more discrete binding detectors.

13 . The method of claim 12 , further comprising distinguishing a first unique fluid mixture from a second unique fluid mixture based on the measured hysteresis effects exhibited by each of the first and second unique fluid mixtures.

14 . The method of claim 12 , wherein applying a voltage comprises applying a voltage to the graphene varactors at multiple discrete voltages across a range of voltages by stepping through the range of voltages in increments from 5 mV to 100 mV.

15 . The method of claim 12 , further comprising characterizing the fluid sample at least in part based on determined hysteresis effects on one or more parameters.

16 . The method of claim 12 , further comprising identifying a disease state of an individual providing the fluid sample based at least in part on determined hysteresis effects.

17 . The method of claim 12 , further comprising identifying a disease state of an individual by matching data gathered from analyzing the fluid sample, including data regarding hysteresis effects, against predetermined data patterns corresponding to disease states.

18 . The method of claim 12 , further comprising

determining one or more parameters of capacitance-voltage data of each of the discrete binding detectors resulting from the applied voltage and;

classifying discrete analytes within the fluid sample based on the determined hysteresis effects and the one or more parameters of capacitance-voltage data in combination.

19 . The method of claim 18 , wherein determining the one or more parameters of capacitance-voltage data comprises determining a forward Dirac point voltage for each of the discrete binding detectors resulting from the applied voltage.

Assignments (6)

Continuity (2)

References Cited (400)