Vaccine compositions comprising C-C motif chemokine 22 (CCL22) or fragments thereof
The present disclosure relates to CCL22 as a T cell target in cancer immunosuppression.
1 . A nucleic acid encoding an immunogenically active CCL22 peptide fragment consisting of at the most 50 consecutive residues of SEQ ID NO: 12, wherein said peptide fragment is of at least 25 amino acids and contains a sequence selected from the group consisting of SEQ ID NO: 1, SEQ ID NO: 3, SEQ ID NO: 4, SEQ ID NO: 5, and SEQ ID NO: 11.
2 . The nucleic acid according to claim 1 , wherein the peptide fragment comprises the sequence of SEQ ID NO: 5.
3 . A composition, comprising the nucleic acid of claim 1 .
4 . The composition of claim 3 , wherein the peptide fragment comprises the sequence of SEQ ID NO: 5.
5 . The composition of claim 3 , further comprising a nucleic acid encoding an immunogenically active protein or peptide fragment selected from a protein or peptide fragment, which is not CCL22.
6 . The composition of claim 3 , comprising an adjuvant.
7 . The composition of claim 6 , wherein the adjuvant is selected from the group consisting of bacterial DNA based adjuvants, oil/surfactant based adjuvants, viral dsRNA based adjuvants and imidazoquinolines.
8 . The composition of claim 6 , wherein the adjuvant is a Montanide ISA adjuvant.
9 . The composition of claim 8 , wherein the adjuvant is Montanide ISA 51 or Montanide ISA 720.
10 . The composition of claim 9 , wherein the adjuvant is Montanide ISA 51.
11 . The composition of claim 6 , wherein the adjuvant is GM-CSF.
12 . The composition of claim 3 , wherein the nucleic acid is comprised within a vector.
13 . The composition of claim 12 , wherein the vector is selected from the group consisting of viral vectors and bacterial vectors.
14 . The composition of claim 12 , wherein the vector furthermore comprises nucleic acids encoding a T-cell stimulatory polypeptide.