Granted Patent B2

US 12,498,318 · App. 18/515,337 · Granted Dec 16, 2025

Inventors: Yi-Hsin Tai (Hsin-Chu, TW); Hsin-Yi Hsieh (Hsin-Chu, TW)

Assignee: VISERA TECHNOLOGIES COMPANY LTD.

G01N21/314G01N21/554G01N2021/1765G01N2021/3166

Abstract

Claims (506)

1 . An imaging method for detecting spectrum shift, comprising:

receiving a first light beam by a first photodiode and a second photodiode to simultaneously obtain a first image signal with a first image intensity from the first photodiode and a second image signal with a second image intensity from the second photodiode,

wherein the first image signal corresponds to the first light beam filtered by a first filter, the second image signal corresponds to the first light beam filtered by a first double-layered filter comprising a second filter overlapped on a third filter, and the third filter has a wavelength range same as a wavelength range of the first filter,

wherein a difference between a peak wavelength of the first image signal and a center wavelength of the first filter is within ±5 nm,

wherein a difference between the peak wavelength of the first image signal and a cut-on/cut-off wavelength of the second filter is within ±5 nm;

receiving a second light beam by the first photodiode and the second photodiode to simultaneously obtain a third image signal with a third image intensity from the first photodiode and a fourth image signal with a fourth image intensity from the second photodiode,

wherein the third image signal corresponds to the second light beam filtered by the first filter, the fourth image signal corresponds to the second light beam filtered by the first double-layered filter; and

determining a spectrum shift from the first light beam to the second light beam by comparing the first image intensity and the second image intensity to the third image intensity and the fourth image intensity.

2 . The imaging method of claim 1 , wherein the second image intensity is half of the first image intensity when receiving the first light beam by the first photodiode and the second photodiode.

3 . The imaging method of claim 1 , wherein a slope of a transition band of the first filter and a slope of a transition band of the second filter are steeper than a slope of the first image signal.

4 . The imaging method of claim 1 , wherein a wavelength range of the first image signal fully falls in the wavelength range of the first filter when receiving the first light beam by the first photodiode and the second photodiode.

5 . The imaging method of claim 1 , wherein the first filter is a short band-pass filter, and the second filter is a long-pass filter or a long band-pass filter.

6 . The imaging method of claim 5 , wherein the short band-pass filter has a bandwidth in a range of 20 nm to 60 nm.

7 . The imaging method of claim 5 , wherein determining the spectrum shift from the first light beam to the second light beam comprises:

obtaining a signal value γ1 and a signal value γ2 from an Equation (I):

γ

=

RI

A

-

(

I

S

-

RI

A

)

I

S

=

2

⁢

RI

A

-

I

S

I

S

,

Equation

⁢

(

I

)

wherein R is an equilibrium parameter, I S and I A are the first image intensity and the second image intensity for the signal value γ1, I S and I A are the third image intensity and the fourth image intensity for the signal value γ2; and

determining the spectrum shift by a difference between the signal value γ1 and the signal value γ2.

8 . The imaging method of claim 7 , wherein the signal value γ1 equals zero when receiving the first light beam by the first photodiode and the second photodiode.

9 . The imaging method of claim 1 , wherein the first filter is a long band-pass filter, and the second filter is a short-pass filter or a short band-pass filter.

10 . The imaging method of claim 9 , wherein determining the spectrum shift from the first light beam to the second light beam comprises:

obtaining a signal value γ1 and a signal value γ2 from an Equation (II):

γ

=

(

I

L

-

RI

B

)

-

RI

B

I

L

=

I

L

-

2

⁢

RI

B

I

L

,

Equation

⁢

(

II

)

wherein R is an equilibrium parameter, I L and I B are the first image intensity and the second image intensity for the signal value γ1, I L and I B are the third image intensity and the fourth image intensity for the signal value γ2; and

determining the spectrum shift by a difference between the signal value γ1 and the signal value γ2.

11 . The imaging method of claim 1 , wherein receiving the first light beam by the first photodiode and the second photodiode further comprises receiving the first light beam by a third photodiode to simultaneously obtain a fifth image signal with a fifth image intensity from the third photodiode, the fifth image signal corresponds to the first light beam filtered by a fourth filter, and the fourth filter has a wavelength range same as a wavelength range of the second filter; and

wherein receiving the second light beam by the first photodiode and the second photodiode further comprises receiving the second light beam by the third photodiode to simultaneously obtain a sixth image signal with a sixth image intensity from the third photodiode, the sixth image signal corresponds to the second light beam filtered by the fourth filter.

12 . The imaging method of claim 11 , wherein the first filter is a short band-pass filter, and the second filter is a long band-pass filter; and

wherein determining the spectrum shift from the first light beam to the second light beam comprises:

obtaining a signal value γ1 and a signal value γ2 from an Equation (III) or an Equation (IV):

γ

=

RI

A

+

(

I

L

-

RI

A

)

-

(

I

S

-

RI

A

)

I

S

+

I

L

-

RI

A

=

I

L

-

I

S

+

RI

A

I

L

+

I

S

-

RI

A

Equation

⁢

(

III

)

γ

=

(

I

L

-

RI

A

)

-

RI

A

-

(

I

S

-

RI

A

)

I

S

+

I

L

-

R

⁢

I

A

=

I

L

-

I

S

-

RI

A

I

L

+

I

S

-

RI

A

,

Equation

⁢

(

IV

)

wherein R is an equilibrium parameter, (I S , I A , I L ) are the first image intensity, the second image intensity, and the fifth image intensity for the signal value γ1, (I S , I A , I L ) are the third image intensity, the fourth image intensity, and the sixth image intensity for the signal value γ2; and

determining the spectrum shift by a difference between the signal value γ1 and the signal value γ2.

13 . The imaging method of claim 12 , wherein the Equation (III) is simplified to an Equation (V) when I L −RI A =0:

γ

=

RI

A

-

(

I

S

-

RI

A

)

I

S

+

I

L

-

RI

A

=

2

⁢

RI

A

-

I

S

I

L

+

I

S

-

RI

A

,

Equation

⁢

(

V

)

and

wherein the Equation (IV) is simplified to an Equation (VI) when I S −RI A =0:

γ

=

(

I

L

-

RI

A

)

-

RI

A

I

S

+

I

L

-

RI

A

=

I

L

-

2

⁢

RI

A

I

L

+

I

S

-

RI

A

.

Equation

⁢

(

VI

)

14 . The imaging method of claim 11 , wherein the first filter is a long band-pass filter, and the second filter is a short band-pass filter; and

wherein determining the spectrum shift from the first light beam to the second light beam comprises:

obtaining a signal value γ1 and a signal value γ2 from an Equation (VII) or an Equation (VIII):

γ

=

(

I

L

-

RI

B

)

-

RI

B

-

(

I

S

-

RI

B

)

I

L

+

I

S

-

RI

B

=

I

L

-

I

S

-

RI

B

I

L

+

I

S

-

RI

B

Equation

⁢

(

VII

)

γ

=

(

I

L

-

RI

B

)

+

RI

B

-

(

I

S

-

RI

B

)

I

L

+

I

S

-

RI

B

=

I

L

-

I

S

+

RI

B

I

L

+

I

S

-

RI

B

,

Equation

⁢

(

VIII

)

wherein R is an equilibrium parameter, (I L , I B , I S ) are the first image intensity, the second image intensity, and the fifth image intensity for the signal value γ1, (I L , I B , I S ) are the third image intensity, the fourth image intensity, and the sixth image intensity for the signal value γ2; and

determining the spectrum shift by a difference between the signal value γ1 and the signal value γ2.

15 . The imaging method of claim 14 , wherein the Equation (VII) is simplified to an Equation (IX) when I S −RI B =0:

γ

=

(

I

L

-

RI

B

)

-

RI

B

I

L

+

I

S

-

RI

B

=

I

L

-

2

⁢

RI

B

I

L

+

I

S

-

RI

B

,

Equation

⁢

(

IX

)

and

wherein the Equation (VIII) is simplified to an Equation (X) when I L −RI B =0:

γ

=

RI

B

-

(

I

S

-

RI

B

)

I

L

+

I

S

-

RI

B

=

2

⁢

RI

B

-

I

S

I

L

+

I

S

-

RI

B

.

Equation

⁢

(

X

)

16 . The imaging method of claim 1 , wherein receiving the first light beam by the first photodiode and the second photodiode further comprises receiving the first light beam by a third photodiode to simultaneously obtain a fifth image signal with a fifth image intensity from the third photodiode, the fifth image signal corresponds to the first light beam filtered by a second double-layered filter comprising a fourth filter overlapped on a fifth filter, and the fifth filter has a wavelength range same as the wavelength range of the first filter; and

wherein receiving the second light beam by the first photodiode and the second photodiode further comprises receiving the second light beam by the third photodiode to simultaneously obtain a sixth image signal with a sixth image intensity from the third photodiode, the sixth image signal corresponds to the second light beam filtered by the second double-layered filter.

17 . The imaging method of claim 16 , wherein the first filter is a center band-pass filter, the second filter is a short-pass filter or a short band-pass filter, and the fourth filter is a long-pass filter or a long band-pass filter.

18 . The imaging method of claim 16 , wherein determining the spectrum shift from the first light beam to the second light beam comprises:

obtaining a signal value γ1 and a signal value γ2 from an Equation (XI):

γ

=

R

⁡

(

I

A

-

I

B

)

I

C

,

Equation

⁢

(

XI

)

wherein R is an equilibrium parameter, (I C , I B , I A ) are the first image intensity, the second image intensity, and the fifth image intensity for the signal value γ1, (I C , I B , I A ) are the third image intensity, the fourth image intensity, and the sixth image intensity for the signal value γ2; and

determining the spectrum shift by a difference between the signal value γ1 and the signal value γ2.

19 . A biosensor for performing the imaging method of claim 1 , comprising:

a photodiode array comprising the first photodiode and the second photodiode;

a filter array comprising the first filter and the first double-layered filter above the photodiode array,

wherein the first photodiode and the second photodiode corresponds to the first filter and the first double-layered filter respectively;

a sample substrate above the filter array; and

a light source above the sample substrate.

20 . The biosensor of claim 19 , wherein the light source is configured to generate a surface plasmon resonance spectrum, and wherein a wavelength range of the light source covers wavelength ranges of the filter array.

Assignments (1)

Continuity (1)

References Cited (19)