Chitosan porous structure-based magnetically actuated microrobot
The present invention relates to a porous structure-based magnetically actuated microrobot and a fabricating method therefor, wherein the porous structure-based magnetically actuated microrobot is based on a natural polymer having biocompatibility and biodegradability, so that the precise targeting of the porous microrobot through the attachment of magnetic nanoparticles and the drug and cell delivery using the porous microrobot can be attained.
1 . A microrobot comprising: a porous film with the pore space diameters of 35 to 130 μm; and magnetic nanoparticles attached to pore spaces of the porous film,
wherein the porous film contains chitosan as a natural polymer,
wherein the magnetic nanoparticles comprise ferumoxytol and collagen type I,
wherein the magnetic nanoparticles are formed through electrostatic binding of ferumoxytol and collagen type I,
wherein the microrobot has fibronectin attached to its inside or surface, and
wherein stem cells or macrophages are attached to the fibronectin attached to the microrobot.
2 . The microrobot of claim 1 , wherein the porous film contains at least one element selected from the group consisting of C, O, and N, which are main ingredients of a polymer.
3 . The microrobot of claim 1 , wherein the diameter of the magnetic nanoparticles is 1 to 1,000 nm.
4 . A method for fabricating a microrobot, the method comprising:
a porous film manufacturing step of manufacturing a porous film contains chitosan as a natural polymer at −15° C. to −10° C.;
a porous structure forming step of processing the porous film to form a porous structure;
a microrobot preparing step of attaching magnetic nanoparticles comprising ferumoxytol and collagen type I and formed through electrostatic binding of ferumoxytol and collagen type I to the porous structure to prepare a microrobot; and
a material loading step of attaching fibronectin to the microrobot and then loading stem cells or macrophages in the microrobot.
5 . The method of claim 4 , wherein in the porous structure forming step, the porous structure is formed by laser processing of the film.