IP Library › Granted Patent US 12,509,677
Granted Patent B2
US 12,509,677 · App. 17/606,292 · Granted Dec 30, 2025

Probiotic strains having increased storage stability

Inventors: Michael Dauner (Wilmington, DE); Gerda Saxer Quance (Wilmington, DE); Steven Keith Williams (Corinth, TX)
Assignee: INTERNATIONAL N&H DENMARK APS
C12N15/01C12N1/205C12R2001/23
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Quick Facts
Patent No.
US 12,509,677
App. No.
17/606,292
Granted
Dec 30, 2025
Kind
B2
Abstract

Evolved strains of Bifidobacterium and Lactobacillus are provided. The evolved strains have probiotic properties and have enhanced storage stability in both wet and dry conditions. The strains of the invention contain key genetic mutations and phenotypic alterations as compared with wild-type.

Claims (1)

1 . A heat evolved strain of Bifidobacterium animalis subsp. lactis deposited with the DSMZ under number DSM 33461 on 4 Mar. 2020 (BBi 6.6).

Assignments (1)
CHANGE OF NAME Recorded Feb 6, 2024
From: DUPONT NUTRITION BIOSCIENCES APS
To: INTERNATIONAL N&H DENMARK APS
Reel/Frame 066494/0814 →
Continuity (2)
Provisional Application 62838985 · Apr 26, 2019
Related Publication 20220204966A1 · Jun 30, 2022
References Cited (16)
US 8741622B2 · Berger et al. · 2014 [cited by applicant]
Chen et al. “Adaptation of Lactococcus lactis to high growth temperature leads to a dramatic increase in acidification rate”. Scientific Reports vol. 5, Article No. 14199 (2015). doi: 10.1038/srep14199 (Year: 2015). [cited by examiner]
Dong et al. [cited by examiner]
Stress responses and genetic variation in bacteria. Mutation Research/Fundamental and Molecular Mechanisms of Mutagenesis, vol. 569, Issues 1-2, 2005, pp. 3-11. (Year: 2005). [cited by examiner]
Simpson et al. [cited by examiner]
[cited by examiner]
Bhavsar et al. The other lives of ribosomal proteins. Hum Genomics. Jun. 2010;4(5):327-44. (Year: 2010). [cited by examiner]
The Leibniz Institute. Bifidobacterium thermacidophilum DSM 15837. URL: www.dsmz.de/collection/catalogue/details/culture/DSM-15837. Accessed Jun. 11, 2025. (Year: 2000). [cited by examiner]
Biavati, B. and Mattarelli, P. (2015). Bifidobacterium †,‡. In Bergey's Manual of Systematics of Archaea and Bacteria (eds M.E. Trujillo, S. Dedysh, P. DeVos, B. Hedlund, P. Kämpfer, F.A. Rainey and W.B. Whitman). (Year… [cited by examiner]
Rasinkangas et al. ( [cited by examiner]
STAHL, et al., “Complete Genome Sequences of Probiotic Strains [cited by applicant]
Lalicic-Petronijevic, et al., “Viability of probiotic strains Lactobacillus addophilus NCFM® and Biftdobacterium lactis HN019 and their impact on sensory and rheological properties of milk and dark chocolates during sto… [cited by applicant]
Gueimonde, et al. “Enhancing probiotic stability in industrial processes”, FFF Probiotics, vol. 23 (2012). [cited by applicant]
Chen, et al., “Adaptation of Lactococcus lactis to high growth temperature leads to a dramatic increase i acidification rate”, Scientific Reports, pp. 1-15 (2015). [cited by applicant]
Dettman, et al., “Evolutionary insight from whole-genome sequencing of experimentally evolved microbes” Molecular Ecology, vol. 21, pp. 2058-2077 (2012). [cited by applicant]
Barrick, et al., “Genome dynamics during experimental evolution” Nature Reviews, vol. 14, pp. 827-839 (2013). [cited by applicant]