IP Library › Granted Patent US 12,527,882
Granted Patent B2
US 12,527,882 · App. 15/662,190 · Granted Jan 20, 2026

Immolative cell-penetrating complexes for nucleic acid delivery

Inventors: Robert M. Waymouth (Palo Alto, CA); Paul Wender (Palo Alto, CA); Jessica R. Vargas (Palo Alto, CA); Tim R. Blake (Palo Alto, CA); Colin J. McKinlay (Palo Alto, CA); Ronald Levy (Palo Alto, CA); Ole Audun Werner Haabeth (Palo Alto, CA); Nancy Benner (Palo Alto, CA); Katherine Near (Palo Alto, CA)
Assignee: The Board of Trustees of the Leland Stanford Junior University
A61K48/0041A61K47/593A61K47/6907A61K47/6931C12N15/115C12N15/85C12N15/88A61K47/6883C12N15/1135
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Quick Facts
Patent No.
US 12,527,882
App. No.
15/662,190
Granted
Jan 20, 2026
Kind
B2
Abstract

There are provided herein, inter alia, complexes, compositions and methods for the delivery of therapeutic, diagnostic and imaging agents, including nucleic acid, into a cell. The complexes, compositions and methods may facilitate complexation, protection, delivery and release of oligonucleotides and polyanionic cargos into target cells, tissues, and organs both in vitro and in vivo.

Claims (42)

1 . A cationic amphipathic polymer having the formula:

wherein

Ring A is a substituted or unsubstituted cycloalkyl, substituted or unsubstituted heterocycloalkyl, substituted or unsubstituted aryl, or substituted or unsubstituted heteroaryl;

CART comprises a poly(alpha aminoester) immolation domain (IM) which has the formula:

-L 1 -[(LP 1 ) z1 -(IM) z2 -(LP 2 ) z3 ] z4 -L 2 -R 2A

wherein,

R 2A is hydrogen, halogen, —CCl 3 , —CBr 3 , —CF 3 , —CI 3 , CHCl 2 , —CHBr 2 , —CHF 2 , —CHI 2 , —CH 2 Cl, —CH 2 Br, —CH 2 F, —CH 2 I, —CN, —OH, —NH 2 , —COOH, —CONH 2 , —NO 2 , —SH, —SO 3 H, —SO 4 H, —SO 2 NH 2 , —NHNH 2 , —ONH 2 , —NHC(O)NHNH 2 , —NHC(O)NH 2 , —NHSO 2 H, —NHC(O)H, —NHC(O)OH, —NHOH, —OCCl 3 , —OCF 3 , —OCBr 3 , —OCI 3 , —OCHCl 2 , —OCHBr 2 , —OCHI 2 , —OCHF 2 , —OCH 2 Cl, —OCH 2 Br, —OCH 2 I, —OCH 2 F, substituted or unsubstituted alkyl, substituted or unsubstituted heteroalkyl, substituted or unsubstituted cycloalkyl, substituted or unsubstituted heterocycloalkyl, substituted or unsubstituted aryl, or substituted or unsubstituted heteroaryl;

L 1 and L 2 are independently a bond, C(O)O—, —O—, —S—, —NH—, —C(O)NH—, —NHC(O)—, —S(O) 2 —, —S(O)NH—, —NHC(O)NH—, substituted or unsubstituted alkylene, substituted or unsubstituted heteroalkylene, substituted or unsubstituted cycloalkylene, substituted or unsubstituted heterocycloalkylene, substituted or unsubstituted arylene, or substituted or unsubstituted heteroarylene;

LP 1 and LP 2 are independently a bond or a lipophilic polymer domain, wherein at least one of LP 1 or LP 2 is a lipophilic polymer domain;

z5 is an integer from 1 to 10;

z1 and z3 are independently integers from 0 to 30 and at least one of z1 and z3 is not 0;

z4 is an integer from 1 to 100; and

z2 is from 2 to 50;

wherein the lipophilic polymer domain independently has the formula:

and

R 20 is independently hydrogen, unbranched C 1 -C 30 alkyl, which may be fully saturated, mono- or polyunsaturated, or

 and

wherein the poly(alpha aminoester) immolation domain independently has the formula:

2 . The cationic amphipathic polymer of claim 1 , wherein the cationic amphipathic polymer has the formula:

3 . The cationic amphipathic polymer of claim 1 , wherein L 1 is —CH 2 —O—, substituted or unsubstituted alkylene, substituted or unsubstituted heteroalkylene, substituted or unsubstituted cycloalkylene, substituted or unsubstituted heterocycloalkylene, substituted or unsubstituted arylene, or substituted or unsubstituted heteroarylene.

4 . The cationic amphipathic polymer of claim 3 , wherein L 1 is

—CH 2 —O—,

5 . The cationic amphipathic polymer of claim 1 , wherein R20 is independently hydrogen, a stearyl moiety, an oleyl moiety, a linoleyl moiety, a dodecyl moiety, or a nonenyl moiety.

6 . A cationic amphipathic polymer having the formula

wherein:

R 1A is a phenyl,

R 20 is independently hydrogen, stearyl, oleyl, linoleyl, dodecyl, nonenyl, or

z1 is from 2 to 25, and z2 is from 2 to 40.

7 . A cationic amphipathic polymer having the formula:

wherein R 1A is a phenyl substituted with a methoxy linker.

8 . The cationic amphipathic polymer of claim 1 , wherein the polymer is complexed with a nucleic acid.

9 . The cationic amphipathic polymer of claim 8 , wherein the nucleic acid is RNA or DNA.

10 . The cationic amphipathic polymer of claim 9 , wherein the nucleic acid is messenger RNA, small interference RNA, short hairpin RNA, micro RNA, guide RNA, CRISPR RNA, transactivating RNA, plasmid DNA, minicircle DNA, or genomic DNA.

11 . A nanoparticle composition comprising a plurality of the cationic amphipathic polymer of claim 8 .

12 . A pharmaceutical composition comprising the nanoparticle composition of claim 11 or the cationic amphipathic polymer of claim 8 .

13 . A vaccine composition comprising the nanoparticle composition of claim 11 or the cationic amphipathic polymer of claim 8 , and optionally an immunological adjuvant.

14 . The cationic amphipathic polymer of claim 1 , wherein the cationic amphipathic polymer has the formula:

15 . The cationic amphipathic polymer of claim 1 , wherein the polymer is complexed with mRNA.

16 . The cationic amphipathic polymer of claim 6 , wherein the polymer is complexed with mRNA.

17 . The cationic amphipathic polymer of claim 7 , wherein the polymer is complexed with mRNA.

18 . A pharmaceutical composition comprising the cationic amphipathic polymer of claim 16 .

19 . A pharmaceutical composition comprising the cationic amphipathic polymer of claim 17 .

Assignments (1)
ASSIGNMENT OF ASSIGNOR'S INTEREST Recorded Sep 12, 2017
From: WAYMOUTH, ROBERT M.; WENDER, PAUL; VARGAS, JESSICA R.; BLAKE, TIM R.; MCKINLAY, COLIN; LEVY, RONALD; HAABETH, OLE AUDUN WERNER; BENNER, NANCY; NEAR, KATHERINE
To: THE BOARD OF TRUSTEES OF THE LELAND STANFORD JUNIOR UNIVERSITY
Reel/Frame 043565/0598 →
Continuity (2)
Provisional Application 62367555 · Jul 27, 2016
Related Publication 20180028688A1 · Feb 1, 2018
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