Organic nitrogenous fertilizers and methods of making the same
View Patent ↗A method for producing a liquid nitrogen fertilizer from protein-rich plant materials, such as soybeans. The method includes extracting nitrogenous compounds through an alkaline solution under controlled temperature and pH conditions, followed by enzymatic hydrolysis using proteases to break down proteins into amino acids and oligopeptides. The fertilizer composition may include additional plant-beneficial compounds, such as isoflavones, phenols, and phytoalexins, enhancing plant growth, nutrient uptake, and disease resistance. The method maximizes recovery through iterative mechanical separation and optional hydrolysis of solid fractions. The resulting organic liquid fertilizer is suitable for use in sustainable and organic farming applications, providing a nutrient-rich, environmentally friendly alternative to conventional chemical fertilizers.
1 . A method of producing a liquid nitrogen fertilizer from organic material, comprising:
a. performing an extraction process on a slurry of plant material in water at a predetermined temperature and alkaline pH range for a predetermined period;
b. separating a first liquid portion of the slurry from solid material in said slurry;
c. performing an enzymatic digestion step on the first liquid portion using one or more proteases at a predetermined temperature and pH range for a predetermined period to produce a first hydrolysate; and
d. concentrating the first hydrolysate to yield a nitrogen-rich fertilizer composition including about 2% to about 12% w/w free amino acids.
2 . The method of claim 1 , further comprising subjecting the plant material to a defatting process to yield defatted organic material prior to preparing said slurry and creating a slurry of the defatted organic material.
3 . The method of claim 1 , further comprising performing a hydrolysis step on solid material removed from the extraction step in water at a predetermined temperature and pH range for a predetermined period.
4 . The method of claim 3 , further comprising separating a second liquid hydrolysate of the resulting hydrolysis product of the hydrolysis step.
5 . The method of claim 4 , further comprising combining the first hydrolysate with the second hydrolysate, placing the combined hydrolysates in a chamber and heating the composition at a predetermined pH, at a predetermined temperature, and for a predetermined period of time to further hydrolyze peptides present in the combined hydrolysates.
6 . The method of claim 4 , further comprising passing the combined the first and second hydrolysates through an osmosis system to remove clean water and provide a concentrated hydrolysate extract that provides an amino acid rich composition.
7 . The method of claim 1 , wherein the plant material is selected from a group consisting of soy beans, soy bean products, clover, duckweed, cottonseed, lentils, chickpeas, Spirulina, hemp seeds, chia seeds, and alfalfa.
8 . The method of claim 1 , further comprising the steps of:
a. drying the solid material removed from the extraction step;
b. placing the dried solid plant material into a pellet mill; and
c. producing a separate pellet fertilizer from the dried solid plant material.
9 . The method of claim 1 , wherein the composition comprises isoflavones in a range of about 0.01 w/w to about 0.2% w/w.
10 . The method of claim 1 , wherein at least 50% of the composition is composed of free amino acids and oligopeptides of less than 2000 Da.
11 . The method of claim 1 , wherein said composition comprises phytoalexins and phenolic compounds that provide a synergistic enhancement in nitrogen-use efficiency in a plant to which the fertilizer composition is applied.
12 . A method for producing a concentrated amino acid fertilizer from an enzymatic hydrolysate of proteinaceous plant material, comprising:
a. performing an extraction process on a slurry of plant material in water at a predetermined temperature and alkaline pH range for a predetermined period;
b. separating a first liquid portion of the slurry from solid material in said slurry;
c. hydrolyzing the first liquid portion derived from the plant material by adding protease enzymes, at a temperature in a range of about 48° C. to about 60° C., and at a pH in a range of about 8 to about 9; and
d. heating the resulting hydrolysate to a temperature in a range of about 75° C. to about 90° C. to deactivate the protease enzymes.
13 . The method of claim 12 , further comprising subjecting the hydrolysate to a reverse osmosis process to remove water and concentrate the nitrogenous compounds.
14 . The method of claim 12 , recovering the concentrated liquid fertilizer containing about 2% to about 12% amino acids by weight.
15 . The method of claim 12 , further comprising adding organic acids to stabilize the pH of the concentrated liquid fertilizer in a range of about 6.5 to about 7.5.
16 . A method of producing a liquid nitrogen fertilizer from organic material, comprising:
a. performing a non-enzymatic extraction process on a slurry of plant material in water at a predetermined temperature and alkaline pH range for a predetermined period;
b. separating a first liquid portion of the slurry from solid material in said slurry;
c. performing an enzymatic digestion step on the first liquid portion using one or more proteases at a predetermined temperature and pH range for a predetermined period to produce a first hydrolysate; and
d. concentrating the first hydrolysate to yield a nitrogen-rich fertilizer composition including about at least 50% w/w of the composition consists of free amino acids and oligopeptides of less than 2000 Da.
17 . The method of claim 16 , further comprising subjecting the plant material to a defatting process to yield defatted organic material prior to preparing said slurry and creating a slurry of the defatted organic material.
18 . The method of claim 16 , further comprising performing a hydrolysis step on solid material removed from the extraction step in water at a predetermined temperature and pH range for a predetermined period.
19 . The method of claim 18 , further comprising separating a second liquid hydrolysate of the resulting hydrolysis product of the hydrolysis step.
20 . The method of claim 19 , further comprising combining the first hydrolysate with the second hydrolysate, placing the combined hydrolysates in a chamber and heating the composition at a predetermined pH, at a predetermined temperature, and for a predetermined period of time to further hydrolyze peptides present in the combined hydrolysates.