Method to prepare a soy hydrolysate product with a high concentration of free amino acids and method of using the same
The present development is a method for producing a high concentration free amino acid soy hydrolysate composition and a method for using the resulting hydrolysate composition. The high concentration free amino acid soy hydrolysate is used to enhance the microbe population found in soil and wastewater. The enhanced microbe population can promote plant growth, particularly for use in brownfields and similar reclamation sites or on turf grasses, and can promote the digestion of sewage solids.
1. A method of treating wastewater, the method comprising:
(a) providing a free amino acid soy hydrolysate liquor prepared from a reaction mixture made by reacting soy meal with a diluted hydrochloric acid solution until the pH of the reaction mixture is below pH 1 and concurrently heating the reaction mixture to a temperature of at least 60° C. (140° F.) for a period of time of up to forty-eight hours, and then cooling the reaction mixture to ambient temperature and adding a base to raise the pH of the reaction mixture to at least pH 3, and then filtering the reaction mixture and recovering the free amino acid soy hydrolysate liquor;
(b) providing wastewater containing raw sewage solids for treatment;
(c) applying the free amino acid soy hydrolysate liquor to the wastewater to deliver from 0.01% to 5.00% by mass high concentration free amino acid soy (HCAAS)-hydrolysate solids in a hydrolysate solids in a reaction product mixture after step (c); and
(d) allowing the free amino acid soy hydrolysate liquor to react with the wastewater for a predetermined time period in the reaction product mixture after step (c).
2. The method of claim 1 wherein the reaction mixture is heated to a temperature of between 100° C. (212° F.) and 105° C. (221° F.).
3. The method of claim 1 wherein the reaction mixture is heated for a period of time of from about one hour to about twenty-four hours.
4. The method of claim 1 wherein the base is selected from the group consisting of potassium hydroxide, sodium hydroxide, aqueous ammonia or a combination thereof.
5. The method of claim 1 wherein the free amino acid soy hydrolysate liquor contains about 30% to about 35% by mass dissolved solids.
6. The method of claim 1 wherein the free amino acid soy hydrolysate liquor has about 0.5% to about 5.0% amino nitrogen, by mass.
7. The method of claim 6 wherein the free amino acid soy hydrolysate liquor has about 1.2% to about 2.4% amino nitrogen, by mass.
8. The method of claim 1 wherein the free amino acid soy hydrolysate liquor has a percent dry basis aspartic acid concentration of at least 3% by mass and a percent dry basis glutamic acid concentration of at least 4% by mass.
9. The method of claim 1 wherein the free amino acid soy hydrolysate liquor is allowed to react with the wastewater for up to seven hours.
10. The method of claim 1 wherein the free amino acid soy hydrolysate liquor is mixed with a surfactant, an emulsifier, an antifoaming agent, a dispersant, or a combination thereof.