High flowrate flushing for open port sampling probe
In a sampling system for mass spectrometry, a method and apparatus are set forth for high flow-rate flushing and sample delivery via a sampling probe ( 10 ). The sampling system includes a sampling probe ( 10 ) having a first fluid conduit ( 40 ) with an inlet, a second fluid conduit ( 42 ) with an outlet, and a sampling port fluidly connecting the first fluid conduit ( 40 ) and second fluid conduit ( 42 ). A fluid source ( 50 ) is attached to the inlet and a vacuum source ( 60 ) is attached to the outlet for causing fluid to flow through the first fluid conduit ( 40 ) past the sampling port and exit through the second fluid conduit ( 42 ). A cap ( 90 ) is provided for selectively closing and opening the sampling port. When the cap is removed, thus when the sampling port is open, sample may be introduced into, and captured by fluid flowing through the sampling port. When the cap is in place, thus when the sampling port is closed, a flushing fluid is supplied for flushing the sampling probe ( 10 ).
1 . A sampling system, comprising:
a sampling probe comprising:
a first fluid conduit having an inlet, the first fluid conduit in fluid communication with a second fluid conduit having an outlet, and a sampling port for receiving and capturing sample fluidly connecting the first fluid conduit and the second fluid conduit;
a fluid source for supplying capture fluid attached to the inlet; and,
a cap for selectively closing the sampling port and opening the sampling port to receive a sample.
2 . The sampling system of claim 1 , further comprising an actuator for selectively placing the cap over the sampling port and removing the cap from over the sampling port to selectively close and open the sampling port.
3 . The sampling system of claim 1 , further comprising an actuator for selectively locating the sampling port over the cap and removing the sampling port from the cap to selectively close and open the sampling port.
4 . The sampling system of claim 2 , wherein the actuator is a robotic arm.
5 . The sampling system of claim 1 , further comprising an aperture in the cap for introducing additional fluid when the sampling port is closed.
6 . The sampling system of claim 5 , wherein the aperture is a pin-hole for introducing the additional fluid.
7 . The sampling system of claim 1 , wherein the fluid source includes a pump for introducing the fluid under pressure at a first flow rate.
8 . A method of operating a sampling probe that comprises a first fluid conduit having an inlet, the first fluid conduit in fluid communication with a second fluid conduit having an outlet, and a sampling port for receiving and capturing sample fluidily fluidly connecting the first fluid conduit and the second fluid conduit, the method comprising:
supplying capture fluid to the inlet;
aspirating the capture fluid from the outlet; and,
controlling the flow of the capture fluid through the sampling port using a cap to selectively close the sampling port and open the sampling port to receive a sample.
9 . The method of claim 8 , further comprising selectively placing a cap over the sampling port and removing the cap from the sampling port to selectively close and open the sampling port.
10 . The method of claim 8 , further comprising selectively locating the sampling port on the cap and removing the sampling port from cap to selectively close and open the sampling port.
11 . The method of claim 8 , further comprising introducing additional fluid through an aperture in the cap when the sampling port is closed.
12 . The method of claim 8 , further comprising increasing the flow rate of the capture fluid when the sampling port is closed.
13 . The method of claim 8 , further comprising switching to a second fluid when the flow rate of the capture fluid is increased.
14 . The method of claim 13 , wherein the capture fluid comprises methanol, and the second fluid comprises methanol with 0.1 formic acid.
15 . The method of claim 8 , further comprising an actuator operative to move either the cap or the sampling probe to selectively open and close the sampling port.
16 . A method of sampling, comprising:
providing a sampling probe including a first fluid conduit having an inlet, a second fluid conduit having an outlet, and a sampling port for receiving and capturing sample between the first fluid conduit and second fluid conduit;
supplying a capture fluid at the inlet;
aspirating the capture fluid at the outlet;
opening the sampling port and supplying capture fluid at a first flow rate to capture samples at the sampling port; and
closing the sampling port and supplying a flushing fluid at a second flow rate higher than the first flow rate for flushing the sampling probe.
17 . The method of claim 16 , further comprising selectively locating a cap over the sampling port and separating the cap from the sampling port to selectively open and close the sampling port.
18 . The method of claim 16 , further comprising introducing additional fluid through an aperture in the cap when the sampling port is closed.
19 . The method of claim 16 , wherein the flushing fluid is selected from the group consisting of:
a) the capture fluid;
b) a flushing solvent; and,
c) a combination of the capture fluid and formic acid.
20 . The method of claim 8 , further comprising selectively closing the sampling port, using the cap, to flush the sampling probe.