Fluid management system for an analyzer and/or sorter type flow type particle analyzer using a detachable connector
Fluid management systems for flow type particle analyzers are provided. Aspects of the fluid management systems include a flow cell comprising an input and output; a cuvette comprising an input coupled to the output of the flow cell and further comprising an output; a sample input line for fluidically coupling a sample fluid source to the input of the flow cell; and a fluid supply subsystem configured to alternatively fluidically couple: (a) a primary fluid source; or (b) one or more secondary fluid sources, to the input of the flow cell. Also provided are methods of using flow type particle analyzers having the subject fluid management systems.
1 . A flow type particle analyzer comprising a fluid management system comprising:
a flow cell comprising an input and output;
a cuvette comprising an input coupled to the output of the flow cell and further comprising an output;
a sample input line for fluidically coupling a sample fluid source to the input of the flow cell such that sample fluid may flow from the sample fluid source to the flow cell; and
a fluid supply subsystem comprising a fluid supply subsystem connector, the fluid supply subsystem configured to alternatively:
(a) fluidically couple a primary fluid source; and
(b) fluidically couple one or more secondary fluid sources,
to the input of the flow cell such that fluid may flow from each fluidically coupled fluid source, through each fluid pathway of the fluid supply subsystem, to the flow cell by detachably connecting the fluid supply subsystem connector to one of a primary fluid source connector and a secondary fluid source connector at a time,
wherein each of the primary and secondary fluid source connectors comprises a single connector for mating with a single, shared interface of the fluid supply subsystem connector, such that only one of the fluid source connectors may be connected to the fluid supply subsystem connector at a time.
2 . The flow type particle analyzer of claim 1 , wherein the sample input line is fluidically coupled to a sample fluid source.
3 . The flow type particle analyzer of claim 1 , wherein the fluid supply subsystem fluidically couples the primary fluid source to the input of the flow cell.
4 . The flow type particle analyzer of claim 1 , wherein the primary fluid source comprises a sheath fluid source.
5 . The flow type particle analyzer of claim 1 , wherein the fluid supply subsystem fluidically couples the one or more secondary fluid sources to the input of the flow cell.
6 . The flow type particle analyzer of claim 1 , wherein the one or more secondary fluid sources comprises a cleaning fluid source.
7 . The flow type particle analyzer of claim 1 , wherein the fluid management system comprises a fluid movement device configured to convey one or more secondary fluids from one or more secondary fluid sources to the fluid supply subsystem.
8 . The flow type particle analyzer of claim 1 , wherein the fluid management system comprises a waste management subsystem configured to receive and combine waste fluids from:
(a) one or more system waste fluid sources; and
(b) an analyzer waste fluid collection subsystem, a sorter waste fluid collection subsystem, or a combination thereof
to produce a combined waste fluid.
9 . The flow type particle analyzer of claim 1 , wherein the fluid management system comprises:
an analyzer waste fluid collection subsystem configured to fluidically couple the output of the cuvette to a waste management subsystem;
a sorter waste fluid collection subsystem configured to fluidically couple an output of a sort block coupled to the output of the cuvette to a waste management subsystem; or
a combination thereof.
10 . The flow type particle analyzer of claim 1 , wherein the fluid management system comprises a primary fluid pressure feedback control subsystem configured to control primary fluid pressure based on measured fluid level height and air pressure in the primary fluid source.
11 . The flow type particle analyzer of claim 1 , wherein the fluid management system comprises a sample fluid pressure feedback control subsystem configured to control sample fluid pressure based on measured air pressure in the sample fluid source and a primary fluid pressure control signal.
12 . The flow type particle analyzer of claim 1 , wherein the fluid management system comprises a pressurized air source configured to supply air pressure to a primary fluid source air pressure regulator and a sample fluid source air pressure regulator.
13 . The flow type particle analyzer of claim 1 , wherein the flow type particle analyzer comprises an interrogation source.
14 . The flow type particle analyzer of claim 1 , wherein the flow type particle analyzer comprises an acousto-optic device configured to frequency shift laser light using applied acoustic waves.
15 . The flow type particle analyzer of claim 1 , wherein the flow type particle analyzer comprises a controller configured to apply radiofrequency drive signals to an acousto-optic device to produce angularly deflected laser beams in an output laser beam.
16 . The flow type particle analyzer of claim 1 , wherein the flow type particle analyzer comprises a light detection system.
17 . The flow type particle analyzer of claim 1 , wherein the flow type particle analyzer is a flow cytometer.
18 . The flow type particle analyzer of claim 1 , wherein the primary fluid source is gaseously coupled with a pressurized air source through a primary fluid source air pressure regulator configured to control air pressure in the primary fluid source; and
wherein the secondary fluid source connector is fluidically coupled to a fluid supply pump that is fluidically coupled to the one or more secondary fluid sources such that the pump is configured to flow the one or more secondary fluids from the one or more secondary fluid sources through the fluid supply subsystem to the flow cell.
19 . A method comprising:
flowing fluid in a flow type particle analyzer comprising a fluid management system comprising:
a flow cell comprising an input and output;
a cuvette comprising an input coupled to the output of the flow cell and further comprising an output;
a sample input line fluidically coupling a sample fluid source to the input of the flow cell such that sample fluid may flow from the sample fluid source to the flow cell; and
a fluid supply subsystem comprising a fluid supply subsystem connector, the fluid supply subsystem configured to alternatively:
(a) fluidically couple a primary fluid source; and
(b) fluidically couple one or more secondary fluid sources,
to the input of the flow cell such that fluid may flow from each fluidically coupled fluid source, through each fluid pathway of the fluid supply subsystem, to the flow cell by detachably connecting the fluid supply subsystem connector to one of a primary fluid source connector and a secondary fluid source connector at a time;
wherein the input of the flow cell is fluidically coupled to either the primary fluid source or the one or more second fluid sources, and
wherein each of the primary and secondary fluid source connectors comprises a single connector for mating with a single, shared interface of the fluid supply subsystem connector, such that only one of the fluid source connectors may be connected to the fluid supply subsystem connector at a time.
20 . The method of claim 19 wherein the primary fluid source is gaseously coupled with a pressurized air source through a primary fluid source air pressure regulator configured to control air pressure in the primary fluid source; and
wherein the secondary fluid source connector is fluidically coupled to a fluid supply pump that is fluidically coupled to the one or more secondary fluid sources such that the pump is configured to flow the one or more secondary fluids from the one or more secondary fluid sources through the fluid supply subsystem to the flow cell.