Blood analysis method, control device and blood cell analyzer
The present disclosure provided a blood cell analyzer, a control device and a blood analysis method thereof. In the method, a first reagent is mixed with a sample to obtain a first testing sample, and then a second reagent is mixed with the first testing sample for a further reaction to get a second testing sample for basophil classification and/or HGB measurement. A blood sample may be tested in one reaction cell through time-division multiplexing technology to obtain four groups leukocytes classification result and HGB result by single detection channel. Thus, the structure of the analyzer may be greatly simplified on the premise of guaranteeing the performance of the analyzer, the size and cost of the analyzer may reduce and a performance-price ratio of the analyzer may increase.
1. A blood analysis method for a blood cell analyzer, the blood cell analyzer comprising a reaction cell where a testing sample is prepared from a blood sample, and a detection apparatus which detects the testing sample for leukocyte detection, and said method comprising:
injecting a blood sample and a first reagent into the reaction cell, wherein the first reagent lyses erythrocytes in the blood sample and reacts with leukocytes in the blood sample to obtain a first testing sample;
transferring a part of the first testing sample from the reaction cell to the detection apparatus for leukocyte four-group classification detection;
injecting a second reagent into the reaction cell having a remainder of the first testing sample after transferring the part of the first testing sample, wherein the second reagent further reacts with leukocytes in the remainder of the first testing sample to obtain a second testing sample; and
transferring the second testing sample to the detection apparatus for basophil classification detection.
2. The method according to claim 1 , wherein the first testing sample and the second testing sample are prepared in a common reaction cell.
3. The method according to claim 1 , further comprising measuring the second testing sample for hemoglobin measurement.
4. The method according to claim 1 , further comprising:
acquiring a first detecting information after the leukocyte four-group classification detection is performed in the detection apparatus;
acquiring a second detecting information after the basophil classification detection is performed in the detection apparatus; and
performing leukocyte five-group classification based on the first and the second detecting information.
5. The method according to claim 1 , wherein the leukocyte four-group classification detection and the basophil classification detection are performed in an optical detection apparatus.
6. The method according to claim 1 , wherein the leukocyte four-group classification detection is performed in an optical detection apparatus and the basophil classification detection is performed in an impedance detection apparatus.
7. The method according to claim 1 , further comprising counting leukocytes based on a signal acquired during the leukocyte four-group classification detection or a signal acquired during basophil classification detection.
8. The method according to claim 1 , comprising:
transferring the part of the first testing sample from the reaction cell to the detection apparatus via pipelines of the analyzer, and
cleaning the pipelines and the detection apparatus through which the first testing sample has passed, before transferring the second testing sample for the basophil classification detection via the pipelines.
9. A blood analysis method for a blood cell analyzer, the blood cell analyzer comprising a reaction cell where a testing sample is prepared from a blood sample, and a detection apparatus which detects the testing sample, and said method comprising:
injecting a blood sample and a first reagent in the reaction cell, wherein the first reagent lyses erythrocytes in the blood sample and reacts with leukocytes in the blood sample to obtain a first testing sample;
transferring a part of the first testing sample from the reaction cell to the detection apparatus for leukocyte five-group classification detection;
injecting a second reagent into the reaction cell having a remainder of the first testing sample after transferring the part of the first testing sample, wherein the second reagent further reacts with leukocytes or erythrocytes in the remainder of the first testing sample to obtain a second testing sample; and
measuring the second testing sample for hemoglobin measurement.
10. The method according to claim 9 , further comprising counting leukocytes based on a signal acquired during the leukocyte five-group classification detection.
11. The method according to claim 9 , further comprising transferring a part of the second testing sample to the detection apparatus for leukocyte counting detection.