Granted Patent
B2
US 12,535,492 · App. 18/394,339 · Granted Jan 27, 2026
Mass spectral tissue analysis
Inventors:
Robert Graham Cooks (West Lafayette, IN); Livia Schiavinato Eberlin (Lafayette, IN); Christina Ramires Ferreira (West Lafayette, IN); Allison Lisa Dill (Indianapolis, IN); Demian R. Ifa (West Lafayette, IN)
Assignee:
Purdue Research Foundation
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Abstract
The invention generally relates to mass spectral analysis. In certain embodiments, methods of the invention involve analyzing a lipid containing sample using a mass spectrometry technique, in which the technique utilizes a liquid phase that does not destroy native tissue morphology during analysis.
Claims (33)
1 . A method for assessing a tissue sample for presence of cancerous cells that are associated with brain, kidney, or prostate, the method comprising:
producing mass spectral data using a desorption electrospray ionization (DESI) technique that comprises directing a liquid phase that does not destroy native tissue morphology from a DESI probe onto the tissue sample to thereby desorb one or more compounds from the sample that are analyzed by a mass spectrometer;
receiving the mass spectral data of a tissue sample;
producing a mass spectral image of the tissue sample based on the mass spectral data;
receiving histochemical data of the tissue sample;
producing an optical image of the tissue sample from the histochemical data;
overlaying the mass spectral image of the tissue sample with the optical image of the tissue sample to produce an overlaid image; and
assessing the overlaid image for presence of cancerous cells that are associated with at least one type of cancer selected from the group consisting of brain, kidney, and prostate.
2 . The method according to claim 1 , wherein assessing comprises determining a distribution of the one or more compounds in the tissue sample based on an analysis of the overlaid image.
3 . The method according to claim 2 , further comprising grading the cancerous cells based on the distribution of the one or more compounds in the tissue sample.
4 . The method according to claim 3 , wherein the cancerous cells are indicative of brain cancer.
5 . The method of claim 1 , wherein the liquid phase is a solvent comprising dimethylformamide (DMF), acetonitrile (ACN), or tetrahydrofuran (THF).
6 . The method according to claim 5 , wherein the liquid phase comprises at least one other component.
7 . The method according to claim 6 , wherein the at least one other component is selected from the group consisting of ethanol (EtOH), water (H 2 O), acetonitrile (ACN), and a combination thereof.
8 . The method according to claim 1 , wherein the liquid phase is selected from the group consisting of: acetonitrile (ACN):ethanol (EtOH); methanol (MeOH):chloroform (CHCl 3 ); and acetonitrile (ACN):chloroform (CHCl 3 ).
9 . The method according to claim 1 , wherein the histochemistry data is produced by H&E staining.
10 . The method according to claim 1 , wherein the one or more compounds comprises one or more lipids.
11 . A method for assessing a tissue sample for presence of cancerous cells that are associated with brain, kidney, or prostate, the method comprising:
producing mass spectral data of a tissue sample comprising a cancerous section that is associated with brain, kidney, or prostate using a desorption electrospray ionization (DESI) technique that comprises directing a liquid phase that does not destroy native tissue morphology from a DESI probe onto the tissue sample to thereby desorb one or more compounds from the sample that are analyzed by a mass spectrometer;
receiving the mass spectral data of the tissue sample comprising the cancerous section that is associated with brain, kidney, or prostate;
producing a mass spectral image of the tissue sample comprising the cancerous section based on the mass spectral data;
receiving histochemical data of the tissue sample comprising the cancerous section;
producing an optical image of the tissue sample comprising the cancerous section from the histochemical data;
overlaying the mass spectral image of the tissue sample comprising the cancerous section with the optical image of the tissue sample comprising the cancerous section to produce an overlaid image;
identifying the cancerous section that is associated with brain, kidney, or prostate within the overlaid image; and
assessing the overlaid image to determine a grade of the cancerous section that is associated with brain, kidney, or prostate.
12 . The method according to claim 11 , wherein the mass spectral data is produced using a desorption electrospray ionization (DESI) technique operated in imaging mode.
13 . The method of claim 11 , wherein the liquid phase is a solvent comprising dimethylformamide (DMF), acetonitrile (ACN), or tetrahydrofuran (THF).
14 . The method according to claim 13 , wherein the liquid phase comprises at least one other component.
15 . The method according to claim 14 , wherein the at least one other component is selected from the group consisting of ethanol (EtOH), water (H 2 O), acetonitrile (ACN), and a combination thereof.
16 . The method according to claim 11 , wherein the liquid phase is selected from the group consisting of: acetonitrile (ACN):ethanol (EtOH); methanol (MeOH):chloroform (CHCl 3 ); and acetonitrile (ACN):chloroform (CHCl 3 ).
17 . The method according to claim 11 , wherein the histochemistry data is produced by H&E staining.
18 . The method according to claim 11 , wherein the one or more compounds comprises one or more lipids.
Assignments (1)
ASSIGNMENT OF ASSIGNOR'S INTEREST
Recorded Apr 22, 2024
From: COOKS, ROBERT GRAHAM; EBERLIN, LIVIA SCHIAVINATO; FERREIRA, CHRISTINA RAMIRES; DILL, ALLISON LISA; IFA, DEMIAN R.
To: PURDUE RESEARCH FOUNDATION
Reel/Frame 067181/0420 →
Continuity (6)
Provisional Application
61487363
· May 18, 2011
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