Method for producing microbial oil from labyrinthulid microorganism capable of producing said microbial oil
A method for producing a microbial oil includes genetically modifying a labyrinthulid which belongs to the genus Parietichytrium or the genus Schizochytrium by disrupting and/or silencing a Δ4 desaturase gene, a C20 elongase gene, and/or a C18 elongase gene, or by being transformed with an ω3 desaturase gene; culturing the labyrinthulid; and collecting the microbial oil from the labyrinthulid. The labyrinthulid has an activity of synthesizing DHA via a PUFA-PKS pathway in an amount of not more than 1/100 of a total amount of DHA synthesized in the labyrinthulid or no activity of producing PUFAs via the PUFA-PKS pathway, and the labyrinthulid has an activity of producing PUFAs via an endogenous elongase-desaturase pathway.
1 . A method for producing a microbial oil, comprising:
genetically modifying a labyrinthulid which belongs to the genus Parietichytrium or the genus Schizochytrium by disrupting and/or silencing a Δ4 desaturase gene, a C20 elongase gene, and/or a C18 elongase gene, or by being transformed with an ω3 desaturase gene;
culturing the labyrinthulid; and
collecting the microbial oil from the labyrinthulid,
wherein the labyrinthulid has an activity of synthesizing DHA via a PUFA-PKS pathway in an amount of not more than 1/100 of a total amount of DHA synthesized in the labyrinthulid or no activity of producing PUFAs via the PUFA-PKS pathway, and the labyrinthulid has an activity of producing PUFAs via an endogenous elongase-desaturase pathway.
2 . The method according to claim 1 , wherein the microbial oil comprises an increased amount of DTA as compared with the microbial oil collected from the labyrinthulid whose Δ4 desaturase gene is not disrupted or silenced.
3 . The method according to claim 1 , wherein the microbial oil comprises an increased amount of n-3 DPA as compared with the microbial oil collected from the labyrinthulid whose Δ4 desaturase gene is not disrupted or silenced.
4 . The method according to claim 1 , wherein the microbial oil comprises an increased amount of EPA as compared with the microbial oil collected from the labyrinthulid whose C20 elongase gene is not disrupted or silenced.
5 . The method according to claim 1 , wherein the microbial oil comprises an increased amount of ARA as compared with the microbial oil collected from the labyrinthulid whose C20 elongase gene is not disrupted or silenced.
6 . The method according to claim 1 , wherein the microbial oil comprises an increased amount of STA as compared with the microbial oil collected from the labyrinthulid whose C18 elongase gene is not disrupted or silenced.
7 . The method according to claim 1 , wherein the microbial oil comprises an increased amount of GLA as compared with the microbial oil collected from the labyrinthulid whose C18 elongase gene is not disrupted or silenced.
8 . The method according to claim 1 , wherein the microbial oil comprises an increased amount of EPA as compared with the microbial oil collected from the labyrinthulid which is not transformed with the 03 desaturase gene.
9 . The method according to claim 1 , wherein the microbial oil comprises an increased amount of ETA as compared with the microbial oil collected from the labyrinthulid which is not transformed with the 03 desaturase gene.
10 . The method according to claim 1 , wherein the labyrinthulid belonging to the genus Parietichytrium is Parietichytrium sp. SEK358 (FERM BP-11405), Parietichytrium sarkarianum SEK364 (FERM BP-11298), or Parietichytrium sp. SEK571 (FERM BP 11406), and/or wherein the labyrinthulid belonging to the genus Schizochytrium is Schizochytrium aggregatum ATCC 28209.