Granted Patent
B2
US 12,552,961 · App. 18/635,552 · Granted Feb 17, 2026
Droplet forming devices and methods having fluorous diol additives
Inventors:
Tathagata Mukherjee (San Jose, CA); Eric Anderson (Lafayette, CA); Stefanie Nishimura (Castro Valley, CA)
Assignee:
10x Genomics, Inc.
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Abstract
Crosslinked fluorocarbon surface coating, kits therefor, microfluidic devices having crosslinked fluoropolymer surface coatings, and methods of use for droplet generation are provided. The kits, devices, and their methods have a droplet source region that is coated with a crosslinked fluoropolymer surface coating produced by the reaction of a fluorocarbon silane and a fluorocarbon polyol.
Claims (19)
1 . A microfluidic device comprising:
a first channel or region being coated with a crosslinked fluorocarbon surface coating and having a first depth, a first width, a first proximal end, and a first distal end,
wherein the crosslinked fluorocarbon surface coating is produced by the reaction of a fluorocarbon silane and a fluorocarbon polyol,
wherein the fluorocarbon polyol is a compound of Formula V:
wherein:
V is —(CZ 2 ) n′ —C 1-15 perfluoroalkylene-(CZ 2 ) n′ — or —(CZ 2 ) n′ —(C 1-15 heterofluoroalkylene) n′ —(CZ 2 ) n′ —, wherein each Z is independently H, F, or C 1-6 perfluoroalkyl and each n′ is independently 1, 2, 3, 4, 5, 6 or 7.
2 . The device of claim 1 , wherein V is —(CZ 2 ) n′ —C 1-15 perfluoroalkylene-(CZ 2 ) n′ —.
3 . The device of claim 1 , wherein V is —(CZ 2 ) n′ —(C 1-15 heterofluoroalkylene) n′ -(CZ 2 ) n′ —.
4 . The device of claim 1 , wherein V has the structure of Formula VI:
wherein each n is independently 0, 1, 2, 3, 4, 5, 6, or 7, and m is 1, 2, 3, 4, 5, or 6.
5 . The device of claim 1 , wherein the fluorocarbon silane has the structure of Formula II:
wherein each n is independently 1, 2, 3, 4, 5, 6, or 7; and
m is 1, 2, 3, 4, 5, or 6.
6 . A microfluidic device comprising:
a first channel or region being coated with a crosslinked fluorocarbon surface coating and having a first depth, a first width, a first proximal end, and a first distal end,
wherein the crosslinked fluorocarbon surface coating is produced by the reaction of a fluorocarbon silane and a fluorocarbon polyol,
wherein the fluorocarbon silane has the structure of Formula II:
wherein each n is independently 1, 2, 3, 4, 5, 6, or 7; and
m is 1, 2, 3, 4, 5, or 6.
Assignments (1)
ASSIGNMENT OF ASSIGNOR'S INTEREST
Recorded Apr 29, 2024
From: MUKHERJEE, TATHAGATA; ANDERSON, ERIC; NISHIMURA, STEFANIE
To: 10X GENOMICS, INC.
Reel/Frame 067255/0750 →
Continuity (3)
Continuation
PCTUS2023030441
· Aug 17, 2023
Provisional Application
63371787
· Aug 18, 2022
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