Microcapsules containing natural oil and preparation method therefor
View Patent ↗The present invention relates to microcapsules comprising natural oil and, more specifically, to microcapsules which contain gelatin, natural polymers, oil, and an oil thickener and have enhanced mechanical properties, and a preparation method therefor. The microcapsules comprising natural oil according to the present invention have remarkably enhanced mechanical properties and retention properties, and when co-cultured with cells, the microcapsules provide the effect of inducing maturation of the cells, and thus may be used in various fields of microcarriers, cell cultures, and co-culture systems.
1 . A preparation method of microcapsules, the preparation method comprising:
a step (a) of preparing a gelatin solution containing gelatin, a natural oil, and dextrin palmitate as an oil thickener;
a step (b) of preparing a natural polymer solution;
a step (c) of mixing the gelatin solution of the step (a) and the natural polymer solution of the step (b);
a step (d) of adjusting pH of the mixture prepared in the step (c); and
a step (e) of cooling the pH-adjusted mixture of the step (d) to form microcapsules, each microcapsule having a wall containing gelatin and the natural polymer, surrounding a core containing the natural oil thickened with dextrin palmitate,
wherein the oil thickener is contained in an amount of 5 weight % with respect to the weight of the oil,
wherein the natural polymer is a mixture of arabic gum and hyaluronic acid, and
wherein the arabic gum and the hyaluronic acid are mixed at a weight ratio of 1:1 to 1:9.
2 . The preparation method of claim 1 , wherein the natural oil is one or more selected from the group consisting of olive oil, camellia oil, castor oil, palm oil, Jojoba oil, almond oil, grapeseed oil, herbal oil, rose oil, coconut oil, moringa oil, rice bran oil, apricot kernel oil, sunflower oil, meadowfoam seed oil, Abyssinian oil, and squalane.
3 . The preparation method of claim 1 , wherein the dextrin palmitate is the only oil thickener present.
4 . The preparation method of claim 1 , wherein the microcapsules are suitable for use as carriers for mammalian cell culture.
5 . The preparation method of claim 4 , wherein the microcapsules are suitable to support cell aggregation and the formation of spheroids in the mammalian cell culture.