IP Library Granted Patent US 12,582,677
Granted Patent B2
US 12,582,677 · App. 17/227,497 · Granted Mar 24, 2026

Methods for producing retinal tissue and retina-related cell

Inventors: Tokushige Nakano (Osaka, JP); Satoshi Ando (Osaka, JP); Yoshiki Sasai (Wako, JP); Mototsugu Eiraku (Wako, JP)
Assignees: SUMITOMO CHEMICAL COMPANY, LIMITED; RIKEN
A61K35/30A61L27/3633A61L27/3834A61L27/3895C12N5/062C12N5/0621G01N33/5058A61L2430/16C12N2500/84C12N2500/90C12N2501/16C12N2501/415C12N2501/42C12N2506/02C12N2533/90
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Quick Facts
Patent No.
US 12,582,677
App. No.
17/227,497
Granted
Mar 24, 2026
Kind
B2
Abstract

The invention provides a method for producing a retinal tissue by (1) subjecting pluripotent stem cells to floating culture in a serum-free medium containing a substance inhibiting the Wnt signal pathway to form an aggregate of pluripotent stem cells, (2) subjecting the aggregate to floating culture in a serum-free medium containing a basement membrane preparation, and then (3) subjecting the aggregate to floating culture in a serumcontaining medium. The invention also provides a method for producing an optic-cup-like structure, a method for producing a retinal pigment epithelium, and a method for producing a retinal layer-specific neural cell.

Claims (12)

1 . An artificial cell aggregate consisting of a human retinal tissue, with Brn3 and TuJ1-positive ganglion cells in the lowermost layer of the retinal tissue, Crx and Recoverin-positive photoreceptor-precursor cells in the outermost layer and intermediate layer of the retinal tissue, and interneuron progenitor cells between the outermost layer and the lowermost layer of the retinal tissue, wherein the lowermost layer, intermediate layer, and outermost layer form a layered structure, and Crx and Recoverin-positive photoreceptor-precursor cells are accumulated in the outermost layer.

2 . The cell aggregate according to claim 1 , wherein the interneuron progenitor cells comprise Chx10-positive bipolar cells.

3 . The cell aggregate according to claim 1 , which comprises no less than 70% of RAX-positive cells.

4 . The cell aggregate according to claim 1 , which comprises Nrl-positive cells and Rxr-gamma-positive cells.

5 . The cell aggregate according to claim 1 , which comprises rod cells and cone cells.

6 . The cell aggregate according to claim 1 , which comprises Ptfla-positive cells.

7 . The cell aggregate according to claim 1 , which comprises horizontal cells or amacrine cells.

8 . The cell aggregate according to claim 1 , which are differentiated in vitro from pluripotent stem cells.

9 . A retinal tissue which is cut out from the cell aggregate according to claim 1 .

10 . A cell culture comprising the cell aggregate according to claim 1 and a medium.

11 . A transplant comprising the cell aggregate according to claim 1 and a medium.

12 . A transplant comprising the retinal tissue according to claim 9 and a medium.

Priority Claims (8)
JP 2011-258209 · Nov 25, 2011 · national
JP 2011-258210 · Nov 25, 2011 · national
JP 2011-258211 · Nov 25, 2011 · national
JP 2011-258212 · Nov 25, 2011 · national
JP 2012-043080 · Feb 29, 2012 · national
JP 2012-043081 · Feb 29, 2012 · national
JP 2012-043082 · Feb 29, 2012 · national
JP 2012-043083 · Feb 29, 2012 · national
Continuity (2)
Continuation 14360534
Related Publication 20210228645A1 · Jul 29, 2021
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