IP Library › Granted Patent US 12,588,686
Granted Patent B2
US 12,588,686 · App. 17/646,177 · Granted Mar 31, 2026

Method for producing modified pea protein

Inventors: Hiroshi Takayanagi (Kawasaki, JP); Hiroyuki Nakagoshi (Kawasaki, JP); Rikiya Ishida (Kawasaki, JP)
Assignees: AJINOMOTO CO., INC.; AMANO ENZYME INC.
A23J1/148C07K14/415
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Quick Facts
Patent No.
US 12,588,686
App. No.
17/646,177
Granted
Mar 31, 2026
Kind
B2
Abstract

Methods including performing (I) a step of adding an acid to a water dispersion of a pea ground product and collecting the precipitate, and (II) a step of conducting an enzyme reaction by adding protein deamidase, in this order or reverse order, are useful for improving the solubility of a modified pea protein.

Claims (28)

1 . A method for producing a modified pea protein, comprising:

(A1) dry pulverizing a pea into a pea ground product and mixing the pea ground product with water, or wet pulverizing a pea in water, to obtain a water dispersion of a ground pea product,

(A2) adding an acid to (i) a solution obtained by removing solid substances from the water dispersion of a ground pea product obtained in step (A1) or (ii) the water dispersion obtained in step (A1), to obtain a precipitate and collecting the precipitate, and

(A3) conducting an enzyme reaction by adding protein deamidase to the precipitate obtained in step (A2), or adding protein deamidase after dissolving the precipitate obtained in step (A2), to obtain the modified pea protein,

wherein the protein deamidase is protein glutaminase or protein asparaginase,

wherein the protein deamidase is added in an amount of 1 to 1000 units per 1 g of the protein, and

wherein the protein glutaminase or the protein asparaginase alone is used as the enzyme.

2 . A method for producing a modified pea protein, comprising:

(B1) dry pulverizing a pea into a pea ground product and mixing the pea ground product with water, or wet pulverizing a pea in water, to obtain a water dispersion of a pea ground product,

(B2) adding protein deamidase to (i) a solution obtained by removing solid substances from the water dispersion of a ground pea product obtained in step (B1) or (ii) the water dispersion obtained in step (B1), to obtain an enzymatic reaction mixture, and

(B3) adding an acid to the enzymatic reaction mixture obtained in step (B2), to obtain a precipitate, and collecting the precipitate, which contains the modified pea protein,

wherein the protein deamidase is protein glutaminase or protein asparaginase,

wherein the protein deamidase is added in an amount of 1 to 1000 units per 1 g of the protein, and

wherein the protein glutaminase or the protein asparaginase alone is used as the enzyme.

3 . A method for improving solubility of a pea protein, comprising:

(A1) dry pulverizing a pea into a pea ground product and mixing the pea ground product with water, or wet pulverizing a pea in water,

(A2) adding an acid to (i) a solution obtained by removing solid substances from the water dispersion of a pea ground product obtained in step (A1) or (ii) the water dispersion obtained in step (A1), to obtain a precipitate, and collecting the precipitate, and

(A3) conducting an enzyme reaction by adding protein deamidase to the precipitate obtained in step (A2), or adding protein deamidase after dissolving the precipitate obtained in step (A2), to obtain the modified pea protein with improved solubility,

wherein the protein deamidase is protein glutaminase or protein asparaginase,

wherein the protein deamidase is added in an amount of 1 to 1000 units per 1 g of the protein, and

wherein the protein glutaminase or the protein asparaginase alone is used as the enzyme.

4 . A method for improving solubility of a pea protein, comprising:

(B1) dry pulverizing a pea into a pea ground product and mixing the pea ground product with water, or wet pulverizing a pea in water, to obtain a water dispersion of a pea ground product,

(B2) adding protein deamidase to (i) a solution obtained by removing solid substances from the water dispersion of a pea ground product obtained in step (B1) or (ii) the water dispersion obtained in step (B1), to obtain an enzymatic reaction mixture, and

(B3) adding an acid to the enzymatic reaction mixture obtained in step (B2), to obtain a precipitate, and collecting the precipitate, which contains the modified pea protein with improved solubility,

wherein the protein deamidase is protein glutaminase or protein asparaginase,

wherein the protein deamidase is added in an amount of 1 to 1000 units per 1 g of the protein, and

wherein the protein glutaminase or the protein asparaginase alone is used as the enzyme.

Assignments (2)
UNDIVIDED INTEREST ASSIGNMENT Recorded Sep 7, 2022
From: AJINOMOTO CO., INC.
To: AMANO ENZYME INC.
Reel/Frame 061387/0966 →
ASSIGNMENT OF ASSIGNOR'S INTEREST Recorded Sep 6, 2022
From: TAKAYANAGI, HIROSHI; NAKAGOSHI, HIROYUKI; ISHIDA, RIKIYA
To: AJINOMOTO CO., INC.
Reel/Frame 060998/0804 →
Priority Claims (2)
JP 2019-124836 · Jul 3, 2019 · national
JP 2020-054915 · Mar 25, 2020 · national
Continuity (2)
Continuation PCTJP2020026034 · Jul 2, 2020
Related Publication 20220117259A1 · Apr 21, 2022
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