Granted Patent B2

US 12,606,835 · App. 17/855,849 · Granted Apr 21, 2026

Inventors: Xiangzhao Mao (Qingdao, CN); Zhen Liu (Qingdao, CN); Zhuoning Cao (Qingdao, CN)

Assignee: OCEAN UNIVERSRRY OF CHINA

C12N15/70C07K14/43595C12N9/0069C12N9/16C12N9/88C12Y113/12007C12Y402/02003C12N2830/002

Abstract

Claims (12)

1 . An auto-induction regulatory system based on quorum sensing, wherein: the system comprises luxI, luxR and egfp, a promoter for controlling the expression of luxI and luxR, and a promoter for controlling the expression of egfp, wherein, the promoter for controlling the expression of luxI and luxR is P luxI , P BB Or P J23100 ; and the promoter for controlling the expression of egfp is P luxI (C-77T), where the nucleotide sequence of said P luxI (C-77T) is as set forth in SEQ ID NO: 5.

2 . The auto-induction regulatory system based on quorum sensing according to claim 1 , wherein:

the nucleotide sequence of said P luxI is as set forth in SEQ ID NO: 1;

the nucleotide sequence of said P BB is as set forth in SEQ ID NO: 2;

the nucleotide sequence of said P J23100 is as set forth in SEQ ID NO: 3;

and the nucleotide sequences of said luxI, luxR and egfp are as set forth in SEQ ID NO: 9, SEQ ID NO: 10, and SEQ ID NO: 11.

3 . An engineered bacterium containing the auto-induction regulatory system based on quorum sensing according to claim 1 .

4 . A recombinant expression vector, whose structure is P luxI -luxI-luxR-P luxI (C-77T)-est7, wherein, the nucleotide sequence of said P luxI is as set forth in SEQ ID NO: 1;

the nucleotide sequence of said P luxI (C-77T) is as set forth in SEQ ID NO: 5;

the nucleotide sequence of said luxI is as set forth in SEQ ID NO: 9, and the nucleotide sequence of said luxR is as set forth in SEQ ID NO: 10;

and the nucleotide sequence of said est7 is as set forth in SEQ ID NO: 13.

5 . A recombinant engineered bacterium comprising the recombinant expression vector according to claim 4 in its genome.

Priority Claims (1)

Continuity (1)

References Cited (9)