Detection of mycobacterium on growth media
View Patent ↗To determine the presence of Mycobacterium in an environment, a sample from the environment can be plated onto a growth medium that is selective for Mycobacterium . Prior to plating, the sample may be treated with a compound containing sodium dodecyl sulfate containing glycine hydrochloride. Plating may occur immediately after the addition of the compound or after a standing period. The agar based growth medium can include a high concentration of crystal violet, in excess of 0.5 μg/ml. Mycobacterium colonies will generally appear white while other colonies will generally appear stained purple or another color.
1 . A method for determining the presence of Mycobacterium in a sample, comprising:
(A) obtaining a sample from an environment;
(B) treating the sample with a compound containing sodium dodecyl sulfate and glycine hydrochloride, wherein the compound comprises 1-5 mM glycine hydrochloride and 0.1%-1.0% sodium dodecyl sulfate;
(C) plating the sample treated with sodium dodecyl sulfate containing glycine hydrochloride onto a growing medium;
(D) incubating a plated sample for an incubation period; and
(E) after the incubation period, inspecting one or more bacterial growth colonies to determine the presence of Mycobacterium in the environment.
2 . The method of claim 1 comprising treating the sample with sodium dodecyl sulfate containing glycine hydrochloride for at least 4 minutes at room temperature.
3 . The method of claim 1 wherein the sample is plated onto the growth medium immediately after treatment with the compound.