Thermophilic and thermoacidophilic metabolism genes and enzymes from
Isolated and/or purified polypeptides and nucleic acid sequences encoding polypeptides from Alicyclobacillus acidocaldarius are provided. Further provided are methods for modulating or altering metabolism in a cell using isolated and/or purified polypeptides and nucleic acid sequences from Alicyclobacillus acidocaldarius.
1. An expression vector comprising an isolated polynucleotide encoding a polypeptide having at least 90% sequence identity to SEQ ID No. 443 and a nucleotide sequence heterologous to the polynucleotide.
2. The expression vector of claim 1 , wherein the expression vector comprises an isolated polynucleotide having at least 95% identity to SEQ ID No. 444.
3. The expression vector of claim 1 , wherein the encoded polypeptide has 3-hydroxybutyryl-CoA dehydratase enzymatic activity.
4. A method of modulating or altering metabolism in a cell, the method comprising:
providing the expression vector of claim 3 to the cell and expressing the encoded polypeptide in the cell.
5. The method according to claim 4 , further comprising glycosylating, or otherwise post-translationally modifying the encoded peptide in the cell.
6. An expression vector comprising an isolated polynucleotide encoding a polypeptide having at least 95% sequence identity to SEQ ID No. 443 and a nucleotide sequence heterologous to the polynucleotide.
7. The expression vector of claim 6 , wherein the encoded polypeptide has 3-hydroxybutvrvl-CoA dehydratase enzymatic activity.
8. The expression vector of claim 6 , wherein the expression vector comprises an isolated polynucleotide having at least 95% identity to SEQ ID No. 444.