Magnetic lysis method and device
A method for lysing cells is disclosed. The method includes stirring cells with a magnetic stir element in the presence of a plurality of cell lysis beads at a speed sufficient to lyse the cells. Also disclosed is a device for lysing cells. The device includes a container having a magnetic stir element and a plurality of cell lysis beads disposed therein. The container is dimensioned to allow rotation of the magnetic stir element inside the container.
1. A device for lysing cells, comprising:
a chamber comprising a magnetic stir element and a plurality of beads, wherein the chamber is dimensioned to allow rotation of the magnetic stir element inside the chamber and wherein the chamber is placed on a base; and
a magnet in the proximity of the chamber and the magnetic stir element inside the chamber,
wherein the magnet is positioned alongside or above the base of the chamber such that the magnet produces a varying magnetic field by rotating along an axis that extends along the longest dimension of the magnet and not perpendicular to the base of the chamber, and wherein the axis passes through the center of the magnet.
2. The device of claim 1 , further comprising a temperature control module that controls the temperature of the chamber.
3. The device of claim 1 , wherein the plurality of beads comprises a material selected from the group consisting of plastics, glass, ceramics, metals and mixtures thereof.
4. The device of claim 1 , wherein the plurality of beads comprises silica beads.
5. The device of claim 1 , wherein each of the plurality of beads has a diameter within the range of between 10 μm and 1000 μm.
6. The device of claim 1 , wherein the magnetic stir element comprises a metal or an alloy.
7. The device of claim 1 , wherein the magnetic stir element comprises an alloy core coated with a polymer.
8. The device of claim 7 , wherein the alloy core comprises neodymium iron boron or samarium cobalt and/or is coated with a biocompatible polymer.
9. The device of claim 8 , wherein the biocompatible polymer is polytetrafluoroethylene (PTFE) or parylene.
10. The device of claim 1 , wherein the magnetic stir element is bar-shaped, cylinder-shaped, cross-shaped, V-shaped, triangular, rectangular, trapezoidal, a rod or disc-shaped, or two-pronged tuning fork shaped.
11. The device of claim 1 , wherein the magnetic stir element is coated with a chemically inert material.
12. The device of claim 11 , wherein the chemically inert material is a polymer, glass, or ceramic.
13. The device of claim 1 , wherein the longest dimension of the stir element is slightly smaller than the diameter of the chamber.