Fungal endophytes for improved crop yields and protection from pests
The invention provides a synthetic combination of a crop and at least one fungal endophyte, wherein the crop is a host plant of the endophyte. Provided are also methods and compositions for producing such synthetic combinations. The endophyte reproduces and enhances the agronomic characteristics of the crop. Methods for inoculating the host plant with the endophyte, for propagating the host-endophyte combination, and for detecting the presence of the endophyte and of its metabolites within a host plant are also described.
1. A method for improving a trait in a cotton plant, the method comprising: contacting an agricultural seed of said cotton plant with a formulation comprising purified filamentous, spore-forming, facultative fungal endophytes of at least one species, wherein the endophytes are Eurotiomycetes capable of producing substances that are beneficial to plants or detrimental to pests or both, wherein the at least one species of endophytes comprise Paecilomyces sp., and wherein the endophytes are present in the formulation in an amount effective to modulate the colonization frequencies of the endophytes of genus Alternaria that are native to the cotton plant, and to provide a benefit to the cotton plant compared to a reference cotton plant grown from a seed untreated with the Paecilomyces sp. facultative fungal endophytes.
2. The method of claim 1 , wherein the benefit is an improved agronomic property selected from the group consisting of increased biomass, increased tillering, increased root mass, increased flowering, increased yield, increased water use efficiency, reduction of yield loss, altered plant height, decreased time to emergence, increased seedling height, increased root length, increased chlorophyll levels, retention of developing flowers, retention of developing fruits, altered phytohormone levels, and enhanced resistance to environmental stress relative to a reference plant.
3. The method of claim 1 , wherein the endophytes belong to the sub-class Eurotiomycetidae.