Reduced gluten grains and compositions thereof
Plants with reduced gluten grains and compositions thereof are disclosed herein.
1. Wheat grain comprising: a wheat prolamin box binding factor (“WPBF”) gene with one or more non-transgenic human induced mutations encoding a WPBF protein comprising an amino acid sequence having 95% or greater identity to SEQ ID NO:3, or having 95% or greater identity to SEQ ID NO:6, or having 95% or greater identity to SEQ ID NO:9, wherein the grain comprises an increase in high molecular weight glutenin content as compared to grain from a wild type plant.
2. The wheat grain of claim 1 , wherein the grain further comprises a reduced gluten content as compared to grain from a wild type plant.
3. The wheat grain of claim 1 , wherein the grain further comprises a reduced gliadin content as compared to grain from a wild type plant.
4. The wheat grain of claim 1 , wherein the grain further comprises a reduced low molecular weight glutenin content as compared to grain from a wild type plant.
5. The wheat grain of claim 1 , wherein the grain further comprises an increase in lysine content as compared to grain from a wild type plant.
6. The wheat grain of claim 1 , wherein the WPBF protein comprises an amino acid sequence having 95% or greater identity to SEQ ID NO: 3.
7. The wheat grain of claim 1 , wherein the WPBF protein comprises an amino acid sequence having 95% or greater identity to SEQ ID NO: 6.
8. The wheat grain of claim 1 , wherein the WPBF protein comprises an amino acid sequence having 95% or greater identity to SEQ ID NO: 9.
9. Flour comprising a cell of the wheat grain of claim 1 .
10. A food or beverage product comprising a cell of the wheat grain of claim 1 .
11. A method of altering glutenin content in a wheat plant comprising;
(a) introducing at least one human induced mutation in a WPBF gene in plant material or plant parts from the wheat plant;
(b) growing the plant material or plant parts to produce progeny wheat plants; and
(c) selecting a progeny wheat plant that produces wheat grain having the one or more non-transgenic human induced mutations in the WPBF gene encoding a WPBF protein comprising an amino acid sequence having 95% or greater identity to SEQ ID NO: 3, or having 95% or greater identity to SEQ ID NO: 6, or having 95% or greater identity to SEQ ID NO: 9, wherein the grain comprises an increase in high molecular weight glutenin content as compared to grain from a wild type plant.
12. The method of claim 11 , wherein the grain further comprises a reduced gluten content as compared to grain from a wild type plant.
13. The method of claim 11 , wherein the grain further comprises a reduced gliadin content as compared to grain from a wild type plant.
14. The method of claim 11 , wherein the grain further comprises a reduced low molecular weight glutenin content as compared to grain from a wild type plant.
15. The method of claim 11 , wherein the grain further comprises an increase in lysine content as compared to grain from a wild type plant.
16. The method of claim 11 , wherein the WPBF gene comprises a nucleic acid sequence having 95% or greater identity to SEQ ID NO: 2.
17. The method of claim 11 , wherein the WPBF gene comprises a nucleic acid sequence having 95% or greater identity to SEQ ID NO: 5.
18. The method of claim 11 , wherein the WPBF gene comprises a nucleic acid sequence having 95% or greater identity to SEQ ID NO: 8.