IP Library › Granted Patent US 12,551,898
Granted Patent B2
US 12,551,898 · App. 17/888,054 · Granted Feb 17, 2026

Test card for assay and method of manufacturing same

Inventors: Ryan Alan Revilla (Costa Mesa, CA); Roy James Heltsley (Foothill Ranch, CA); Steve Hoe Lee (Glendale, CA); Tej Rushikesh Patel (Aliso Viejo, CA)
Assignee: Fluxergy, Inc.
B01L7/52B01L3/5027B01L2200/04B01L2200/0684B01L2200/0689B01L2300/0816B01L2300/0867B01L2300/087B01L2300/0887B01L2300/161B01L2400/049C12Q1/686
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Quick Facts
Patent No.
US 12,551,898
App. No.
17/888,054
Granted
Feb 17, 2026
Kind
B2
Abstract

Disclosed herein is a test card including at least one fluid inlet port, at least one microchannel, and a plurality of target zones, where the test card is configured to perform a plurality of assays in the plurality of target zones. Also described herein is a method of using the test card to perform a plurality of assays on a fluid sample in the plurality of target zones.

Claims (20)

1 . A test card comprising:

at least one fluid inlet port;

a plurality of target zones; and

at least one microchannel fluidically coupling the at least one fluid inlet port and the plurality of target zones;

wherein the test card is configured to perform a plurality of assays in the plurality of target zones, wherein the plurality of assays comprise a plurality of different assays such that the test card is a single test card configured to simultaneously perform a combination of different assays, wherein the plurality of target zones comprise at least two target zones in parallel and at least two target zones in series, wherein the plurality of different assays include at least two of (a) a nucleic acid amplification assay, (b) an immunoassay, (c) an ion selective assay, and (d) an image cytometry assay, or wherein the plurality of different assays include at least two of (a) a capacitance-based assay, (b) a fluorescence-based assay, and (c) a colorimetric-based assay.

2 . The test card according to claim 1 , wherein the plurality of assays include at least two of (a) a nucleic acid amplification assay, (b) an immunoassay, (c) an ion selective assay, or (d) an image cytometry assay.

3 . The test card according to claim 1 , wherein the plurality of assays include at least two of (a) a capacitance-based assay, (b) a fluorescence-based assay, or (c) a colorimetric-based assay.

4 . The test card according to claim 1 , wherein at least two of the plurality of assays are performed in the plurality of target zones at the same time.

5 . The test card according to claim 1 , wherein at least two of the plurality of assays are performed in the plurality of target zones at different times.

6 . The test card according to claim 1 , wherein the plurality of assays include at least three assays.

7 . The test card according to claim 1 , further comprising a plurality of fluid detection zones.

8 . The test card according to claim 1 , further comprising a plurality of sets of electrodes, wherein each set of electrodes is aligned with one of the plurality of target zones, and wherein at least one of the plurality of sets of electrodes is used for heating and/or sensing.

9 . The test card according to claim 1 , wherein the test card is configured such that fluid moves through the at least one microchannel during use.

10 . The test card according to claim 1 , wherein the test card is configured such that fluid is moved through the at least one microchannel from the at least one fluid inlet port to the plurality of target zones after the fluid is received at the at least one fluid inlet port.

11 . The test card according to claim 1 , wherein the test card is configured such that fluid moves through the at least one microchannel upon application of pressure.

12 . The test card according to claim 1 , wherein the at least one fluid inlet port comprises a plurality of fluid inlet ports.

13 . The test card according to claim 1 , further comprising at least one fluid outlet port.

14 . The test card according to claim 1 , wherein the plurality of assays include at least two of (a) a polymerase chain reaction (“PCR”) assay, (b) an enzyme-linked immunosorbent assay (“ELISA”), (c) an agglutination-PCR assay, or (d) an image cytometry assay.

15 . The test card according to claim 1 , wherein the test card is configured for an analysis of at least one assay of the plurality of assays in the plurality of target zones.

16 . The test card according to claim 1 , wherein at least one target zone of the plurality of target zones comprises an analysis port through which at least one assay of the plurality of assays is configured to be analyzed.

Assignments (2)
ASSIGNMENT OF ASSIGNOR'S INTEREST Recorded Aug 19, 2022
From: FLUXERGY, LLC
To: FLUXERGY, INC.
Reel/Frame 061279/0517 →
ASSIGNMENT OF ASSIGNOR'S INTEREST Recorded Aug 18, 2022
From: REVILLA, RYAN ALAN; HELTSLEY, ROY JAMES; LEE, STEVE HOE; PATEL, TEJ RUSHIKESH
To: FLUXERGY, LLC
Reel/Frame 060844/0918 →
Continuity (5)
Continuation 16243485 · Jan 9, 2019
Division 15185661 · Jun 17, 2016
Provisional Application 62187471 · Jul 1, 2015
Provisional Application 62182992 · Jun 22, 2015
Related Publication 20220388006A1 · Dec 8, 2022
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