Systems and methods for multianalyte detection
Provided herein are systems, devices and methods for performing multianalyte detection in a biological sample, such as a human blood sample. Multiwell plates useful for performing multianalyte detection are also provided. The systems, devices and methods provided herein relate to the field of direct-to-consumer diagnostics (DTC diagnostics) and are useful, e.g., for facilitating consumer access to consumer healthcare and consumer wellness information. Other uses of the systems, devices and methods provided herein relate to the fields of medical research and drug discovery.
1 . A method of performing point-of-care assays for a plurality of analytes in a liquid sample, the method being performed within a single diagnostic system, the method comprising:
A. providing
a multiwell plate comprising a plurality of wells, each well containing a portion of the liquid sample, the multiwell plate having a translucent bottom and opaque well walls wherein:
i) a first well contains a first reagent comprising a fluorogenic substrate or a fluorescently labeled binding protein configured to emit a fluorescent signal upon interaction with a first analyte in the sample; and
ii) a second well contains a second reagent comprising a chromogenic substrate configured to produce a detectable absorbance change upon interaction with a second analyte in the sample;
a single light source;
a single CMOS;
a motor;
a platform configured to receive the multiwell plate, the platform comprises platform magnets, platform vertical keys and an opaque film
wherein the opaque film is disposed on an upper surface of the platform;
wherein a peripheral portion of the multiwell plate comprising a plurality of overhang wells including the first well and the second well, overhangs the platform and a selected overhang well from the plurality of overhang wells is within an optical path of the light source and CMOS and is aligned to permit transmission of light through the translucent bottom of the first overhang well without obstruction from the platform or the motor;
the platform vertical keys engaged with a seat by a seat vertical key and platform magnets engaged with the seat by seat magnets in the seat, the seat coupled to the motor; and
a processor operatively connected to the CMOS;
B. moving, under control of the motor, the multiwell plate relative to the single CMOS and single light source, such that the first well of the plurality of overhang wells is aligned with the CMOS and the light source;
C. directing light from the single light source to the translucent bottom of the first well of the plurality of overhang wells and detecting by the CMOS a fluorescence emission generated upon interaction between a first analyte in the sample and the first reagent;
D. moving, under control of the motor, the multiwell plate relative to the single CMOS and single light source, such that the second well of the plurality of overhang wells is aligned with the CMOS and the light source;
E. directing light from the single light source to the translucent bottom of the second well of the plurality of overhang wells and detecting by the CMOS transmitted or absorbed light generated upon interaction between a second analyte in the sample and the second reagent;
F. processing, by the processor, (i) the detected fluorescence emission from the CMOS to generate analyte measurements for the first analyte in the liquid sample and (ii) the transmitted or absorbed light detected from the CMOS to generate analyte measurements for the second analyte in the liquid sample.