Method and device for superresolution optical measurement using singular optics
A method for measuring a position of a fluorophore includes configuring a set of compact light distributions, the set having at least one member, each light distribution characterized by a center, so that there is substantially zero intensity at the center of the set of compact light distributions. The method additionally includes moving the set of compact light distributions in relation to a set of hypothesized positions of the fluorophore, detecting, in a plurality of locations corresponding to the hypothesized set of positions, a set of images; and estimating the position of the fluorophore, by determining from the set of images a set of parameters describing the position of the fluorophore using an inverse problem method.
1. A method for measuring a position of a fluorophore, the method comprising:
configuring a set of compact light distributions, the set having at least one member, each light distribution characterized by a center, so that there is substantially zero intensity at the center of the set of compact light distributions;
moving the set of compact light distributions in relation to a set of hypothesized positions of the fluorophore;
detecting, in a plurality of locations corresponding to the hypothesized set of positions, a set of images; and
estimating the position of the fluorophore, by determining from the set of images a set of parameters describing the position of the fluorophore using an inverse problem method.
2. A method according to claim 1 , wherein the set has a plurality of members, grouped in a single mathematical combination of compact light distributions.
3. A method according to claim 2 , wherein the each of the compact light distributions is a rotated half-moon.
4. A method according to claim 1 , wherein the compact light distribution is a vortex.