Granted Patent B2

US 12,350,637 · App. 18/614,432 · Granted Jul 8, 2025

Inventors: Jason Robert Hyde (Oxford, GB); Pedro Miguel Ortiz Bahamon (Oxford, GB); Clive Gavin Brown (Oxford, GB); Andrew John Heron (Oxford, GB); Paul Raymond Mackett (Oxford, GB)

Assignee: Oxford Nanopore Technologies PLC

B01J19/0046B01L3/5088C12Q1/6869G01N33/48721G01N33/573B01J2219/00313B01J2219/00317B01J2219/00351B01J2219/00585B01J2219/00659B01J2219/00734B01J2219/00736B01L2200/0642B01L2400/086G01N2333/974

Abstract

Claims (17)

1. An apparatus for forming an array of volumes comprising polar medium, the apparatus comprising:

a support that comprises a base and partitions extending from the base, which partitions comprise inner portions that extend from a surface of the base and outer portions that extend from the inner portions, wherein the partitions define compartments, the inner portions defining inner recesses of the compartments, and the outer portions extending outwardly from the inner portions, wherein the outer portions are pillars extending from the inner portions, and wherein the outer portions have surfaces having a patterning that comprises a plurality of indentations;

volumes of polar medium contained in the inner recesses;

a layer comprising polar medium extending across the support, wherein the layer comprising polar medium is in contact with the volumes of polar medium at an interface;

an apolar medium that is present around the pillars; and

membranes comprising amphipathic molecules, wherein the membranes are present at the interfaces between the layer comprising polar medium and the volumes of polar medium.

2. The apparatus of claim 1 , further comprising gaps between the outer portions allowing flow of an apolar medium across the support.

3. The apparatus of claim 1 , wherein the outer portions do not have gaps.

4. The apparatus of claim 1 , wherein the polar medium contained in the inner recesses have a convex surface and the membranes have a concave surface at the interface.

5. The apparatus of claim 1 , wherein the indentations have a depth-to-width aspect ratio of between or equal to 1:1 and 10:1.

6. The apparatus of claim 1 , wherein the indentations have a width of between or equal to 20 microns and 5 microns.

7. The apparatus of claim 1 , wherein the indentations extend along the entire length of the outer portions.

8. The apparatus of claim 1 , wherein the height of the pillars and the height of the inner recesses have an aspect ratio of 1:3 or less.

9. The apparatus of claim 1 , wherein the inner recesses have surfaces having a patterning that comprises a plurality of indentations arranged to retain apolar medium that extend outwardly of the inner recesses.

10. The apparatus of claim 1 , wherein the inner portions of the partitions have a non-circular profile as viewed from the openings of the inner recesses that comprises, around individual compartments, one or more salient portions for constraining a volume comprising polar medium and one or more re-entrant portions providing channels.

11. The apparatus of claim 1 , wherein the inner portions are capable of constraining volumes comprising polar medium contained in neighboring inner recesses from contacting each other.

12. The apparatus of claim 1 , wherein the membranes comprise nanopores.

Assignments (2)

Priority Claims (1)

Continuity (6)

References Cited (332)