IP Library › Granted Patent US 12,235,267
Granted Patent B2
US 12,235,267 · App. 18/642,104 · Granted Feb 25, 2025

Ultra-sensitive detection of molecules or particles using beads or other capture objects

Inventors: David C. Duffy (Arlington, MA); David M. Rissin (Lexington, MA); David R. Walt (Boston, MA); David Fournier (Northborough, MA); Cheuk Kan (Waltham, MA)
Assignee: Quanterix Corporation
G01N33/54393G01N33/54306G01N33/54313
View Patent ↗
Loading inventors, assignments & file history…
Monitor This Case
Get email alerts when status or documents change.
Order Certified Copies
Most orders are placed with the USPTO same day — all within 24 business hours.
Order via The Patent Place →
Pre-filled with this patent's details
Quick Facts
Patent No.
US 12,235,267
App. No.
18/642,104
Granted
Feb 25, 2025
Kind
B2
Abstract

The present invention relates to systems and methods for detecting analyte molecules or particles in a fluid sample and in some cases, determining a measure of the concentration of the molecules or particles in the fluid sample. Methods of the present invention may comprise immobilizing a plurality of analyte molecules or particles with respect to a plurality of capture objects. At least a portion of the plurality of capture objects may be spatially separated into a plurality of locations. A measure of the concentration of analyte molecules in a fluid sample may be determined, at least in part, on the number of reaction vessels comprising an analyte molecule immobilized with respect to a capture object. In some cases, the assay may additionally comprise steps including binding ligands, precursor labeling agents, and/or enzymatic components.

Claims (30)

1. A method for determining a measure of the concentration of analyte molecules or particles in a fluid sample, comprising:

(a) providing capture objects, wherein at least some of the capture objects are associated with at least one type of analyte molecule or particle from the fluid sample and at least some of the capture objects are not associated with the at least one type of analyte molecule or particle;

(b) passing at least some of the capture objects by a detection system;

(c) individually interrogating with the detection system the at least some of the capture objects during the passing;

(d) determining, based at least in part on step (c), a measure indicative of the number or fraction of the capture objects passed by the detection system that are associated with the at least one type of analyte molecule or particle; and

(e) determining a measure of the concentration of the at least one type of analyte molecule or particle in the fluid sample based at least in part on the measure indicative of the number or fraction of the capture objects passed by the detection system that are associated with the at least one type of analyte molecule or particle.

2. The method of claim 1 , wherein at least some of capture objects have a binding surface having affinity for the at least one type of analyte molecule or particle.

3. The method of claim 2 , wherein at least some of the capture objects are beads.

4. The method of claim 1 , wherein a statistically significant fraction of the capture objects provided are associated with the at least one type of analyte molecule or particle and a statistically significant fraction of the capture objects provided are not associated with the at least one type of analyte molecule or particle.

5. The method of claim 1 , wherein the percentage of capture objects provided that are not associated with the at least one type of analyte molecule is at least 30%.

6. The method of claim 1 , wherein the percentage of capture objects provided that are not associated with the at least one type of analyte molecule is at least 50%.

7. The method of claim 1 , wherein the percentage of capture objects provided that are not associated with the at least one type of analyte molecule is at least 70%.

8. The method of claim 1 , wherein:

step (d) comprises determining a measure indicative of the number of the capture objects passed by the detection system that are associated with the at least one type of analyte molecule or particle from the fluid sample, and

step (e) comprises determining a measure of the concentration of the at least one type of analyte molecule or particle in the fluid sample based at least in part on the measure indicative of the number of the capture objects passed by the detection system that are associated with the at least one type of analyte molecule or particle.

9. The method of claim 8 , wherein step (d) further comprises determining a measure indicative of a ratio of the number of the capture objects passed by the detection system that are associated with the at least one type of analyte molecule or particle to the number of the capture objects passed by the detection system that are not associated with the at least one type of analyte molecule or particle.

10. The method of claim 8 , wherein step (e) is based at least in part on a Poisson distribution analysis involving the number of the capture objects passed by the detection system that are associated with the at least one type of analyte molecule or particle.

11. The method of claim 10 , wherein the percentage of capture objects provided that are not associated with the at least one type of analyte molecule is at least 50%.

12. The method of claim 10 , wherein the percentage of capture objects provided that are not associated with the at least one type of analyte molecule is at least 70%.

13. The method of claim 1 , wherein:

step (d) comprises determining a measure indicative of the fraction of the capture objects passed by the detection system that are associated with the at least one type of analyte molecule or particle, and

step (e) comprises determining a measure of the concentration of the at least one type of analyte molecule or particle in the fluid sample based at least in part on the measure indicative of the fraction of the capture objects passed by the detection system that are associated with the at least one type of analyte molecule or particle.

14. The method of claim 1 , wherein at least some of the capture objects passed by the detection system are in single file.

15. The method of claim 1 , wherein the passing comprises feeding the capture objects into a column such that each capture object passes by the detection system.

16. The method of claim 1 , wherein the detection system is an optical detection system.

17. The method of claim 1 , wherein the capture objects comprise or are each contained within a liquid droplet that is immiscible within a fluid in which the droplet is suspended during at least step (b).

18. The method of claim 1 , wherein at least some of the capture objects associated with the at least one type of analyte molecule or particle are further associated with a labeling agent.

19. The method of claim 18 , wherein the labeling agent is generated at least in part by replicating a nucleic acid precursor into multiple copies or by converting a nucleic acid precursor into a nucleic acid that can be detected readily.

20. The method of claim 18 , wherein the labeling agent is generated at least in part by polymerase chain reaction (PCR), rolling circle amplification (RCA), ligation, and/or Loop-Mediated Isothermal Amplification (LAMP).

21. The method of claim 18 , wherein the labeling agent is generated at least in part by RCA.

Assignments (1)
ASSIGNMENT OF ASSIGNOR'S INTEREST Recorded May 17, 2024
From: DUFFY, DAVID C.; RISSIN, DAVID M.; WALT, DAVID R.; FOURNIER, DAVID; KAN, CHEUK
To: QUANTERIX CORPORATION
Reel/Frame 067444/0292 →
Continuity (7)
Continuation 16906257 · Jun 19, 2020
Continuation 15487889 · Apr 14, 2017
Continuation 15265996 · Sep 15, 2016
Continuation 13530979 · Jun 22, 2012
Continuation 12731130 · Mar 24, 2010
Provisional Application 61309141 · Mar 1, 2010
Related Publication 20240295552A1 · Sep 5, 2024
References Cited (400)
US 3712986A · Collings · 1973 [cited by applicant]
US 4200110A · Peterson et al. · 1980 [cited by applicant]
US 4232119A · Carlsson et al. · 1980 [cited by applicant]
US 4631211A · Houghten · 1986 [cited by applicant]
US 4780421A · Kameda et al. · 1988 [cited by applicant]
US 4883642A · Bisconte · 1989 [cited by applicant]
US 4907037A · Boisde et al. · 1990 [cited by applicant]
US 4924870A · Wlodarczyk et al. · 1990 [cited by applicant]
US 4962037A · Jett et al. · 1990 [cited by applicant]
US 5026159A · Allen et al. · 1991 [cited by applicant]
US 5028535A · Buechler et al. · 1991 [cited by applicant]
US 5089391A · Buechler et al. · 1992 [cited by applicant]
US 5091300A · Hurni et al. · 1992 [cited by applicant]
US 5108961A · Zhong et al. · 1992 [cited by applicant]
US 5152816A · Berkey · 1992 [cited by applicant]
US 5190857A · Allen et al. · 1993 [cited by applicant]
US 5196306A · Bobrow et al. · 1993 [cited by applicant]
US 5244636A · Walt et al. · 1993 [cited by applicant]
US 5244813A · Walt et al. · 1993 [cited by applicant]
US 5250264A · Walt et al. · 1993 [cited by applicant]
US 5252494A · Walt · 1993 [cited by applicant]
US 5298741A · Walt et al. · 1994 [cited by applicant]
US 5315375A · Allen · 1994 [cited by applicant]
US 5320814A · Walt et al. · 1994 [cited by applicant]
US 5329461A · Allen et al. · 1994 [cited by applicant]
US 5374395A · Robinson et al. · 1994 [cited by applicant]
US 5405783A · Pirrung et al. · 1995 [cited by applicant]
US 5445934A · Fodor et al. · 1995 [cited by applicant]
US 5468846A · Ichikawa et al. · 1995 [cited by applicant]
US 5488567A · Allen et al. · 1996 [cited by applicant]
US 5512490A · Walt et al. · 1996 [cited by applicant]
US 5532138A · Singh et al. · 1996 [cited by applicant]
US 5532379A · Fujimoto · 1996 [cited by applicant]
US 5545531A · Rava et al. · 1996 [cited by applicant]
US 5583001A · Bobrow et al. · 1996 [cited by applicant]
US 5620850A · Bamdad et al. · 1997 [cited by applicant]
US 5633972A · Walt et al. · 1997 [cited by applicant]
US 5641634A · Mandecki · 1997 [cited by applicant]
US 5690894A · Pinkel et al. · 1997 [cited by applicant]
US 5700637A · Southern · 1997 [cited by applicant]
US 5731158A · Bobrow et al. · 1998 [cited by applicant]
US 5770455A · Cargill et al. · 1998 [cited by applicant]
US 5807522A · Brown et al. · 1998 [cited by applicant]
US 5814524A · Walt et al. · 1998 [cited by applicant]
US 5854033A · Lizardi · 1998 [cited by applicant]
US 5858648A · Steel · 1999 [cited by examiner]
US 5885529A · Babson et al. · 1999 [cited by applicant]
US 5900481A · Lough et al. · 1999 [cited by applicant]
US 5981180A · Chandler et al. · 1999 [cited by applicant]
US 6001564A · Bergeron et al. · 1999 [cited by applicant]
US 6007690A · Nelson et al. · 1999 [cited by applicant]
US 6013445A · Albrecht et al. · 2000 [cited by applicant]
US 6023540A · Walt et al. · 2000 [cited by applicant]
US 6133436A · Koster et al. · 2000 [cited by applicant]
US 6143495A · Lizardi et al. · 2000 [cited by applicant]
US 6143496A · Brown et al. · 2000 [cited by applicant]
US 6156270A · Buechler · 2000 [cited by applicant]
US 6174695B1 · Hammock et al. · 2001 [cited by applicant]
US 6210910B1 · Walt et al. · 2001 [cited by applicant]
US 6266459B1 · Walt et al. · 2001 [cited by applicant]
US 6285807B1 · Walt et al. · 2001 [cited by applicant]
US 6327410B1 · Walt et al. · 2001 [cited by applicant]
US 6329139B1 · Nova et al. · 2001 [cited by applicant]
US 6368874B1 · Gallop et al. · 2002 [cited by applicant]
US 6377721B1 · Walt et al. · 2002 [cited by applicant]
US 6388746B1 · Eriksson et al. · 2002 [cited by applicant]
US 6396995B1 · Stuelpnagel et al. · 2002 [cited by applicant]
US 6406845B1 · Walt et al. · 2002 [cited by applicant]
US 6432630B1 · Blankenstein · 2002 [cited by applicant]
US 6450203B1 · Backhouse et al. · 2002 [cited by applicant]
US 6482593B2 · Walt et al. · 2002 [cited by applicant]
US 6558928B1 · Landegren · 2003 [cited by applicant]
US 6573051B2 · Alsmadi et al. · 2003 [cited by applicant]
US 6582908B2 · Fodor et al. · 2003 [cited by applicant]
US 6586193B2 · Yguerabide et al. · 2003 [cited by applicant]
US 6589736B1 · Rothschild et al. · 2003 [cited by applicant]
US 6599331B2 · Chandler et al. · 2003 [cited by applicant]
US 6602702B1 · McDevitt et al. · 2003 [cited by applicant]
US 6620584B1 · Chee et al. · 2003 [cited by applicant]
US 6635452B1 · Monforte et al. · 2003 [cited by applicant]
US 6667159B1 · Walt et al. · 2003 [cited by applicant]
US 6713309B1 · Anderson et al. · 2004 [cited by applicant]
US 6714303B2 · Ivarsson · 2004 [cited by applicant]
US 6821449B2 · Caplen et al. · 2004 [cited by applicant]
US 6838051B2 · Marquiss et al. · 2005 [cited by applicant]
US 6858394B1 · Chee et al. · 2005 [cited by applicant]
US 6859570B2 · Walt et al. · 2005 [cited by applicant]
US 6878345B1 · Astle · 2005 [cited by applicant]
US 6884357B2 · Siddiqi · 2005 [cited by applicant]
US 6887431B1 · Vann et al. · 2005 [cited by applicant]
US 6929924B2 · Bouanani et al. · 2005 [cited by applicant]
US 6942968B1 · Dickinson et al. · 2005 [cited by applicant]
US 6943034B1 · Winkler et al. · 2005 [cited by applicant]
US 6991939B2 · Walt et al. · 2006 [cited by applicant]
US 6999657B2 · Walt · 2006 [cited by applicant]
US 7041481B2 · Anderson et al. · 2006 [cited by applicant]
US 7056746B2 · Seul et al. · 2006 [cited by applicant]
US 7060431B2 · Chee et al. · 2006 [cited by applicant]
US 7115884B1 · Walt et al. · 2006 [cited by applicant]
US 7211390B2 · Rothberg et al. · 2007 [cited by applicant]
US 7214640B2 · Margetts · 2007 [cited by applicant]
US 7244559B2 · Rothberg et al. · 2007 [cited by applicant]
US 7250267B2 · Walt et al. · 2007 [cited by applicant]
US 7264929B2 · Rothberg et al. · 2007 [cited by applicant]
US 7348181B2 · Walt et al. · 2008 [cited by applicant]
US 7480433B2 · Walt et al. · 2009 [cited by applicant]
US 7553619B2 · Kumar et al. · 2009 [cited by applicant]
US 7572581B2 · Gelfand et al. · 2009 [cited by applicant]
US 7651841B2 · Song et al. · 2010 [cited by applicant]
US 7759062B2 · Allawi et al. · 2010 [cited by applicant]
US 7776553B2 · Love et al. · 2010 [cited by applicant]
US 7838250B1 · Goix et al. · 2010 [cited by applicant]
US 8043834B2 · Abarzua et al. · 2011 [cited by applicant]
US 8093064B2 · Shah et al. · 2012 [cited by applicant]
US 8222047B2 · Duffy et al. · 2012 [cited by applicant]
US 8236574B2 · Duffy et al. · 2012 [cited by applicant]
US 8415171B2 · Rissin et al. · 2013 [cited by applicant]
US 8460878B2 · Walt et al. · 2013 [cited by applicant]
US 8460879B2 · Walt et al. · 2013 [cited by applicant]
US 8492098B2 · Walt et al. · 2013 [cited by applicant]
US 8592221B2 · Fraden et al. · 2013 [cited by applicant]
US 8846415B2 · Duffy et al. · 2014 [cited by applicant]
US 9110025B2 · Rissin · 2015 [cited by examiner]
US 9310360B2 · Duffy et al. · 2016 [cited by applicant]
US 9333163B2 · Farokhzad et al. · 2016 [cited by applicant]
US 9395359B2 · Walt et al. · 2016 [cited by applicant]
US 9482622B2 · Sato et al. · 2016 [cited by applicant]
US 9482662B2 · Duffy et al. · 2016 [cited by applicant]
US 9551663B2 · Rissin et al. · 2017 [cited by applicant]
US 9678068B2 · Duffy et al. · 2017 [cited by applicant]
US 9709559B2 · Banerjee et al. · 2017 [cited by applicant]
US 9809838B2 · Walt et al. · 2017 [cited by applicant]
US 9846155B2 · Rissin et al. · 2017 [cited by applicant]
US 9932626B2 · Duffy et al. · 2018 [cited by applicant]
US 9952237B2 · Fournier et al. · 2018 [cited by applicant]
US 10261089B2 · Walt et al. · 2019 [cited by applicant]
US 10357772B2 · Fraden et al. · 2019 [cited by applicant]
US 10393759B2 · Wilson et al. · 2019 [cited by applicant]
US 10640814B2 · Duffy et al. · 2020 [cited by applicant]
US 10725032B2 · Duffy et al. · 2020 [cited by applicant]
US 10960397B2 · Fraden et al. · 2021 [cited by applicant]
US 10989713B2 · Rissin · 2021 [cited by examiner]
US 11112415B2 · Fournier et al. · 2021 [cited by applicant]
US 11187702B2 · Link et al. · 2021 [cited by applicant]
US 11275092B2 · Wilson et al. · 2022 [cited by applicant]
US 11434264B2 · Pollock et al. · 2022 [cited by applicant]
US 11619631B2 · Duffy et al. · 2023 [cited by applicant]
US 11874279B2 · Walt et al. · 2024 [cited by applicant]
US 12019072B2 · Rissin et al. · 2024 [cited by applicant]
US 20010005584A1 · Matsuno et al. · 2001 [cited by applicant]
US 20020009391A1 · Marquiss et al. · 2002 [cited by applicant]
US 20020081714A1 · Jain et al. · 2002 [cited by applicant]
US 20020090650A1 · Empedocles et al. · 2002 [cited by applicant]
US 20020122612A1 · Walt et al. · 2002 [cited by applicant]
US 20020192649A1 · Lizardi · 2002 [cited by applicant]
US 20030008313A1 · Wiltshire · 2003 [cited by applicant]
US 20030027126A1 · Walt et al. · 2003 [cited by applicant]
US 20030040129A1 · Shah · 2003 [cited by applicant]
US 20030091475A1 · Yu et al. · 2003 [cited by applicant]
US 20030096227A1 · Shinitzky et al. · 2003 [cited by applicant]
US 20030096243A1 · Busa · 2003 [cited by applicant]
US 20030104361A1 · Weininger et al. · 2003 [cited by applicant]
US 20030143555A1 · Bourget et al. · 2003 [cited by applicant]
US 20030143580A1 · Straus et al. · 2003 [cited by applicant]
US 20030198573A1 · Forood et al. · 2003 [cited by applicant]
US 20040002154A1 · Palsson · 2004 [cited by applicant]
US 20040009614A1 · Ahn et al. · 2004 [cited by applicant]
US 20040018561A1 · Decrescenzo et al. · 2004 [cited by applicant]
US 20040038426A1 · Manalis · 2004 [cited by applicant]
US 20040043502A1 · Song et al. · 2004 [cited by applicant]
US 20040053322A1 · McDevitt et al. · 2004 [cited by applicant]
US 20040071599A1 · Rusch et al. · 2004 [cited by applicant]
US 20040072278A1 · Chou et al. · 2004 [cited by applicant]
US 20040086426A1 · Vann et al. · 2004 [cited by applicant]
US 20040091939A1 · Cheung et al. · 2004 [cited by applicant]
US 20040101918A1 · Cauci · 2004 [cited by applicant]
US 20040106112A1 · Nilsson et al. · 2004 [cited by applicant]
US 20040142386A1 · Rigler et al. · 2004 [cited by applicant]
US 20040248103A1 · Feaver et al. · 2004 [cited by applicant]
US 20040248325A1 · Bukusoglu · 2004 [cited by applicant]
US 20040253624A1 · Smith et al. · 2004 [cited by applicant]
US 20040259237A1 · Kellogg et al. · 2004 [cited by applicant]
US 20050037356A1 · Gullberg et al. · 2005 [cited by applicant]
US 20050053996A1 · Tong · 2005 [cited by applicant]
US 20050112634A1 · Woudenberg et al. · 2005 [cited by applicant]
US 20050112655A1 · Banerjee et al. · 2005 [cited by applicant]
US 20050129581A1 · McBride et al. · 2005 [cited by applicant]
US 20050130173A1 · Leamon et al. · 2005 [cited by applicant]
US 20050130188A1 · Walt · 2005 [cited by examiner]
US 20050131650A1 · Andersson et al. · 2005 [cited by applicant]
US 20050164289A1 · Quate et al. · 2005 [cited by applicant]
US 20050220675A1 · Reed et al. · 2005 [cited by applicant]
US 20050221281A1 · Ho · 2005 [cited by applicant]
US 20050226780A1 · Sandell et al. · 2005 [cited by applicant]
US 20050244308A1 · Tanaami et al. · 2005 [cited by applicant]
US 20050255491A1 · Lee et al. · 2005 [cited by applicant]
US 20050266433A1 · Kapur et al. · 2005 [cited by applicant]
US 20050287526A1 · Landegren et al. · 2005 [cited by applicant]
US 20060006067A1 · Unger · 2006 [cited by applicant]
US 20060013543A1 · Walt et al. · 2006 [cited by applicant]
US 20060019319A1 · Billadeau et al. · 2006 [cited by applicant]
US 20060040297A1 · Leamon et al. · 2006 [cited by applicant]
US 20060068409A1 · Phan et al. · 2006 [cited by applicant]
US 20060078998A1 · Puskas et al. · 2006 [cited by applicant]
US 20060084183A1 · Henricksen · 2006 [cited by applicant]
US 20060139635A1 · Kersey et al. · 2006 [cited by applicant]
US 20070040095A1 · Walt et al. · 2007 [cited by applicant]
US 20070059754A1 · Kordunsky et al. · 2007 [cited by applicant]
US 20070074972A1 · Nassef et al. · 2007 [cited by applicant]
US 20070116607A1 · Wang et al. · 2007 [cited by applicant]
US 20070125942A1 · Kido · 2007 [cited by applicant]
US 20070184456A1 · Chee et al. · 2007 [cited by applicant]
US 20070259381A1 · Rissin et al. · 2007 [cited by applicant]
US 20070259385A1 · Rissin et al. · 2007 [cited by applicant]
US 20070259448A1 · Rissin et al. · 2007 [cited by applicant]
US 20080032324A1 · Walt et al. · 2008 [cited by applicant]
US 20080039343A1 · Guire et al. · 2008 [cited by applicant]
US 20080064113A1 · Goix et al. · 2008 [cited by applicant]
US 20080206751A1 · Squirrell et al. · 2008 [cited by applicant]
US 20080248962A1 · Kim et al. · 2008 [cited by applicant]
US 20080254482A1 · Mattoon et al. · 2008 [cited by applicant]
US 20080269069A1 · Bacher et al. · 2008 [cited by applicant]
US 20090036324A1 · Fan et al. · 2009 [cited by applicant]
US 20090087860A1 · Todd et al. · 2009 [cited by applicant]
US 20090142755A1 · Albitar · 2009 [cited by applicant]
US 20090149341A1 · Walt et al. · 2009 [cited by applicant]
US 20090156425A1 · Walt et al. · 2009 [cited by applicant]
US 20090170728A1 · Walt et al. · 2009 [cited by applicant]
US 20090239308A1 · Dube et al. · 2009 [cited by applicant]
US 20090246795A1 · Hayashi et al. · 2009 [cited by applicant]
US 20090254180A1 · Pazanowski et al. · 2009 [cited by applicant]
US 20090283407A1 · Shah et al. · 2009 [cited by applicant]
US 20090289834A1 · Devensky · 2009 [cited by applicant]
US 20090307772A1 · Markham et al. · 2009 [cited by applicant]
US 20100022414A1 · Link · 2010 [cited by examiner]
US 20100028953A1 · Koch et al. · 2010 [cited by applicant]
US 20100028985A1 · Hanafusa et al. · 2010 [cited by applicant]
US 20100047773A1 · Koch et al. · 2010 [cited by applicant]
US 20100075355A1 · Duffy et al. · 2010 [cited by applicant]
US 20100075407A1 · Duffy et al. · 2010 [cited by applicant]
US 20100075439A1 · Duffy et al. · 2010 [cited by applicant]
US 20100075862A1 · Duffy et al. · 2010 [cited by applicant]
US 20100140289A1 · Knobel et al. · 2010 [cited by applicant]
US 20100173394A1 · Colston, Jr. et al. · 2010 [cited by applicant]
US 20100189338A1 · Lin et al. · 2010 [cited by applicant]
US 20100192573A1 · Hamilton et al. · 2010 [cited by applicant]
US 20100204335A1 · Beddingfield et al. · 2010 [cited by applicant]
US 20100225913A1 · Trainer · 2010 [cited by examiner]
US 20100227379A1 · Wo et al. · 2010 [cited by applicant]
US 20100233734A1 · Hobbs · 2010 [cited by applicant]
US 20100258441A1 · Sista et al. · 2010 [cited by applicant]
US 20100329929A1 · Goix · 2010 [cited by examiner]
US 20110037463A1 · Bertacco · 2011 [cited by examiner]
US 20110097723A1 · Liu et al. · 2011 [cited by applicant]
US 20110183330A1 · Lo et al. · 2011 [cited by applicant]
US 20110195852A1 · Walt et al. · 2011 [cited by applicant]
US 20110212462A1 · Duffy et al. · 2011 [cited by applicant]
US 20110212537A1 · Rissin et al. · 2011 [cited by applicant]
US 20110212848A1 · Duffy et al. · 2011 [cited by applicant]
US 20110223585A1 · Gullberg et al. · 2011 [cited by applicant]
US 20110245097A1 · Rissin et al. · 2011 [cited by applicant]
US 20110275061A1 · Weidemaier et al. · 2011 [cited by applicant]
US 20120180149A1 · Lyerla · 2012 [cited by applicant]
US 20120183967A1 · Dressman et al. · 2012 [cited by applicant]
US 20120196774A1 · Fournier et al. · 2012 [cited by applicant]
US 20120214160A1 · Deng et al. · 2012 [cited by applicant]
US 20120220479A1 · Ericcson et al. · 2012 [cited by applicant]
US 20120277114A1 · Duffy et al. · 2012 [cited by applicant]
US 20120289426A1 · Roos et al. · 2012 [cited by applicant]
US 20120289428A1 · Duffy et al. · 2012 [cited by applicant]
US 20120326104A1 · Kwon et al. · 2012 [cited by applicant]
US 20130165342A1 · Rissin et al. · 2013 [cited by applicant]
US 20130345078A1 · Walt et al. · 2013 [cited by applicant]
US 20140094386A1 · Wilson et al. · 2014 [cited by applicant]
US 20140227720A1 · Wilson et al. · 2014 [cited by applicant]
US 20140302532A1 · Wilson et al. · 2014 [cited by applicant]
US 20150233905A1 · Walt et al. · 2015 [cited by applicant]
US 20150353997A1 · Duffy et al. · 2015 [cited by applicant]
US 20150355182A1 · Rissin et al. · 2015 [cited by applicant]
US 20160123969A1 · Rissin et al. · 2016 [cited by applicant]
US 20160258959A1 · Wilson et al. · 2016 [cited by applicant]
US 20170038390A1 · Walt et al. · 2017 [cited by applicant]
US 20170159104A1 · Walt et al. · 2017 [cited by applicant]
US 20170160292A1 · Wilson et al. · 2017 [cited by applicant]
US 20180003703A1 · Duffy et al. · 2018 [cited by applicant]
US 20180017552A1 · Duffy et al. · 2018 [cited by applicant]
US 20180224451A1 · Rissin et al. · 2018 [cited by applicant]
US 20180306830A1 · Fournier et al. · 2018 [cited by applicant]
US 20180363038A1 · Duffy et al. · 2018 [cited by applicant]
US 20190293655A1 · Walt et al. · 2019 [cited by applicant]
US 20190302109A1 · Duffy et al. · 2019 [cited by applicant]
US 20200032326A1 · Walt et al. · 2020 [cited by applicant]
US 20200123592A1 · Díaz-Mochón et al. · 2020 [cited by applicant]
US 20200124620A1 · Wilson et al. · 2020 [cited by applicant]
US 20200271643A1 · Wilson et al. · 2020 [cited by applicant]
US 20200393457A1 · Duffy et al. · 2020 [cited by applicant]
US 20210311058A1 · Rissin et al. · 2021 [cited by applicant]
US 20220034917A1 · Fournier et al. · 2022 [cited by applicant]
US 20220050108A1 · Link et al. · 2022 [cited by applicant]
US 20220099678A1 · Walt et al. · 2022 [cited by applicant]
US 20220205992A1 · Rissin et al. · 2022 [cited by applicant]
US 20220229074A1 · Wilson et al. · 2022 [cited by applicant]
US 20220244276A1 · Wilson et al. · 2022 [cited by applicant]
US 20230109130A1 · Duffy et al. · 2023 [cited by applicant]
US 20230213510A1 · Duffy et al. · 2023 [cited by applicant]
US 20240159765A1 · Walt et al. · 2024 [cited by applicant]
AU 199956253B2 · 2000 [cited by applicant]
CA 2258745A1 · 1997 [cited by applicant]
CN 1635146A · 2005 [cited by applicant]
CN 1928561A · 2007 [cited by applicant]
CN 1950520A · 2007 [cited by applicant]
CN 101351564A · 2009 [cited by applicant]
CN 101529227A · 2009 [cited by applicant]
CN 101541974A · 2009 [cited by applicant]
DE 19540098A1 · 1997 [cited by applicant]
EP 0805215A2 · 1997 [cited by applicant]
EP 1180679A1 · 2002 [cited by applicant]
EP 1259810A2 · 2002 [cited by applicant]
EP 1721657A1 · 2006 [cited by applicant]
EP 2267451A2 · 2010 [cited by applicant]
GB 2343949A · 2000 [cited by applicant]
GB 2463401A · 2010 [cited by applicant]
JP 2001269196A · 2001 [cited by applicant]
JP 2002506200A · 2002 [cited by applicant]
JP 2002525587A · 2002 [cited by applicant]
JP 2004354164A · 2004 [cited by applicant]
JP 2005518553A · 2005 [cited by applicant]
JP 2006511792A · 2006 [cited by applicant]
WO WO8805533A1 · 1988 [cited by applicant]
WO WO9306121A1 · 1993 [cited by applicant]
WO WO9324517A2 · 1993 [cited by applicant]
WO WO9525116A1 · 1995 [cited by applicant]
WO WO9532425A1 · 1995 [cited by applicant]
WO WO9535506A2 · 1995 [cited by applicant]
WO WO9727326A1 · 1997 [cited by applicant]
WO WO9850782A2 · 1998 [cited by applicant]
WO WO9945357A2 · 1999 [cited by applicant]
WO WO9958948A2 · 1999 [cited by applicant]
WO WO0004372A1 · 2000 [cited by applicant]
WO WO0016101A2 · 2000 [cited by applicant]
WO WO0047996A2 · 2000 [cited by applicant]
WO WO0157520A2 · 2001 [cited by applicant]
WO WO02077646A1 · 2002 [cited by applicant]
WO WO02077291 · 2002 [cited by examiner]
WO WO03054142A2 · 2003 [cited by applicant]
WO WO03073817A1 · 2003 [cited by applicant]
WO WO2004065000A1 · 2004 [cited by applicant]
WO WO2004083443A1 · 2004 [cited by applicant]
WO WO2005019419A2 · 2005 [cited by applicant]
WO WO2005023414A1 · 2005 [cited by applicant]
WO WO2005033283A2 · 2005 [cited by applicant]
WO WO2005054431A2 · 2005 [cited by applicant]
WO WO2006007726A1 · 2006 [cited by applicant]
WO WO2006055739A2 · 2006 [cited by applicant]
WO WO2006078289A2 · 2006 [cited by applicant]
WO WO2006102297A1 · 2006 [cited by applicant]
WO WO2006108180A2 · 2006 [cited by applicant]
WO WO2007044091A2 · 2007 [cited by applicant]
WO WO2007044974A2 · 2007 [cited by applicant]
WO WO2007081385A2 · 2007 [cited by applicant]
WO WO2007081386A2 · 2007 [cited by applicant]
WO WO2007081387A1 · 2007 [cited by applicant]
WO WO2007084192A2 · 2007 [cited by applicant]
WO WO2007098148A2 · 2007 [cited by applicant]
WO WO2007114947A2 · 2007 [cited by applicant]
WO WO2007123763A2 · 2007 [cited by applicant]
WO WO2008048371A2 · 2008 [cited by applicant]
WO WO2009029073A1 · 2009 [cited by applicant]
WO WO2010039180A2 · 2010 [cited by applicant]
WO 2010151329A1 · 2010 [cited by applicant]
WO 2011038241A1 · 2011 [cited by applicant]
WO 2011109364A2 · 2011 [cited by applicant]
WO 2011109372A1 · 2011 [cited by applicant]
WO 2016115256A1 · 2016 [cited by applicant]
WO 2016130923A1 · 2016 [cited by applicant]
WO 2018222585A2 · 2018 [cited by applicant]
WO 2019060607A1 · 2019 [cited by applicant]
Rissin et al., [Digital Concentration Readout of Single Enzyme Molecules Using Femtoliter Arrays and Poisson Statistics], Nano Letters, vol. 6, No. 3, pp. 520-523. [cited by examiner]
Rissin et al., “Digital Readout of Target Binding with Attomole Detection Limits via Enzyme Amplification in Femtoliter Arrays”. J. Am. Chem. Soc. 2006, 128, 6286-6287. [cited by examiner]
EP 21181713.5, Jul. 2, 2024, Office Action. [cited by applicant]
Office Communication mailed for U.S. Appl. No. 12/236,484, filed Sep. 23, 2008, published as US 2010-0075862, which Office Communication is dated Sep. 9, 2010, and claims as pending for U.S. Appl. No. 12/236,484 as of S… [cited by applicant]
Office Communication for U.S. Appl. No. 12/236,484, filed Sep. 23, 2008, published as US 2010-0075862, which Office Communication is dated Apr. 13, 2011, and claims as pending for U.S. Appl. No. 12/236,484 as of Apr. 13… [cited by applicant]
Office Communication for U.S. Appl. No. 12/236,486, filed Sep. 23, 2008, published as US 2010-0075407, which Office Communication is dated Nov. 23, 2011, and claims as pending for for U.S. Appl. No. 12/236,486 as of Nov… [cited by applicant]
Notice of Allowance for U.S. Appl. No. 12/236,486, filed Sep. 23, 2008, published as US 2010-0075407 on Mar. 25, 2010, which Notice of Allowance is dated Mar. 22, 2012, and claims as pending for U.S. Appl. No. 12/236,48… [cited by applicant]
Office Communication for U.S. Appl. No. 13/527,210, filed Jun. 19, 2012, which Office Communication is dated Jun. 20, 2013, and claims as pending for Office Communication for U.S. Appl. No. 13/527,210 as of Jun. 20, 201… [cited by applicant]
Office Communication for U.S. Appl. No. 13/527,210, filed Jun. 19, 2012, which Office Communication is dated Feb. 6, 2014, and claims as pending for Office Communication for U.S. Appl. No. 13/527,210 as of Nov. 20, 2013. [cited by applicant]
Notice of Allowance for U.S. Appl. No. 13/527,210, filed Jun. 19, 2012, which Notice of Allowance is dated May 27, 2014, and claims as pending for U.S. Appl. No. 13/527,210 as of Nov. 20, 2013. [cited by applicant]
Office Communication for U.S. Appl. No. 12/236,488, filed Sep. 23, 2008, published as US 2010-0075439, which Office Communication is dated Aug. 2, 2010, and claims as pending for U.S. Appl. No. 12/236,488 as of Aug. 2, … [cited by applicant]
Office Communication for U.S. Appl. No. 12/731,130, filed Mar. 24, 2010, published as US 2011-0212848 on Sep. 1, 2011, which Office Communication is dated Mar. 28, 2012, and claims as pending for U.S. Appl. No. 12/731,1… [cited by applicant]
Notice of Allowance for U.S. Appl. No. 12/731,130, filed Mar. 24, 2010, published as US 2011-0212848 on Sep. 1, 2011, which Notice of Allowance is dated Jun. 1, 2012, and allowed claims for U.S. Appl. No. 12/731,130 as … [cited by applicant]
Office Communication for U.S. Appl. No. 13/530,979, filed Jun. 22, 2012, which Office Communication is dated Jun. 19, 2015, and claims as pending for U.S. Appl. No. 13/530,979 as of Apr. 5, 2013. [cited by applicant]
Office Communication for U.S. Appl. No. 13/530,979, filed Jun. 22, 2012, which Office Communication is dated Jan. 22, 2016, and claims as pending for U.S. Appl. No. 13/530,979 as of Dec. 9, 2015. [cited by applicant]
Office Communication for U.S. Appl. No. 13/530,979, filed Jun. 22, 2012, which Office Communication is dated Jan. 28, 2016, and claims as pending for U.S. Appl. No. 13/530,979 as of Dec. 9, 2015. [cited by applicant]
Office Communication for U.S. Appl. No. 13/531,061, filed Jun. 22, 2012, which Office Communication is dated Aug. 21, 2015, and claims as pending for U.S. Appl. No. 13/531,061 as of Apr. 5, 2015. [cited by applicant]
Office Communication for U.S. Appl. No. 16/381,614 mailed Jun. 21, 2021 and Claims Pending. [cited by applicant]
Office Action for U.S. Appl. No. 16/381,614 dated Jun. 2, 2022 and claims pending. [cited by applicant]
Notice of Allowance for U.S. Appl. No. 16/381,614 dated Dec. 14, 2022 and allowed claims as of Dec. 14, 2022. [cited by applicant]
Office Communication for U.S. Appl. No. 12/731,135, filed Mar. 24, 2010, published as US 20110212462, which Office Communication is dated May 23, 2013, and claims as pending for U.S. Appl. No. 12/731,135 as of May 23, 2… [cited by applicant]
Office Communication for U.S. Appl. No. 12/731,135, filed Mar. 24, 2010, which Office Communication is dated Apr. 3, 2015, and claims as pending for U.S. Appl. No. 12/731,135. [cited by applicant]
Office Communication for U.S. Appl. No. 12/731,135, filed Mar. 24, 2010, which Office Communication is dated Mar. 29, 2016, and claims as pending for U.S. Appl. No. 12/731,135 as of Sep. 3, 2015. [cited by applicant]
Cited By (2)
US 12,540,950 US 12,571,803