Granted Patent
B2
US 12,410,317 · App. 18/649,499 · Granted Sep 9, 2025
Engineered skin equivalent, method of manufacture thereof and products derived therefrom
C08L89/06C12N5/0625C12N5/0629C12N5/0698C12N11/02C14C3/02C14C13/00C12N2501/155C12N2501/385C12N2502/091C12N2502/094C12N2502/13C12N2506/45C12N2533/30C12N2533/54
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Abstract
Disclosed herein are synthetic leathers, artificial epidermal layers, artificial dermal layers, layered structures, products produced therefrom and methods of producing the same.
Claims (19)
1. A method of forming a tanned synthetic leather, wherein the method comprises:
culturing a fibroblast on a scaffold; and
tanning a collagenous extracellular matrix comprising collagen secreted by the fibroblast to generate the tanned synthetic leather.
2. The method of claim 1 , wherein the scaffold comprises a natural material, a synthetic material, or a combination thereof.
3. The method of claim 2 , wherein the scaffold comprises the natural material.
4. The method of claim 2 , wherein the scaffold comprises the synthetic material.
5. The method of claim 1 , wherein the tanning comprises a vegetable tanning, a chrome tanning, an aldehyde tanning, a syntan tanning, a bacterial tanning, or any combination thereof.
6. The method of claim 1 , wherein a thickness of the tanned synthetic leather is about 0.01 mm to about 5 mm.
7. The method of claim 2 , wherein the fibroblast is derived from a mammal or reptile.
8. The method of claim 7 , wherein the fibroblast is a bovine fibroblast.
9. The method of claim 7 , further comprising forming the tanned synthetic leather into one or more of a watch strap, a belt, a packaging, a shoe, a boot, a footwear, a glove, a clothing, a luggage, a bag, a clutch, a purse, a backpack, a wallet, a saddle, a harness, a whip, or any combination thereof.
10. A composition comprising a cell layer comprising a fibroblast in contact with a scaffold and an extracellular matrix, wherein the extracellular matrix comprises a type I collagen, a type III collagen, or a combination thereof, and wherein the composition is suitable for tanning to form a tanned synthetic leather.
11. The composition of claim 10 , wherein the fibroblast is derived from a mammal or a reptile.
12. The composition of claim 11 , wherein the fibroblast is a bovine fibroblast.
13. The composition of claim 10 , wherein the composition further comprises an extracellular matrix.
14. The composition of claim 10 , wherein the scaffold comprises a natural material, a synthetic material, or a combination thereof.
15. The composition of claim 14 , wherein the scaffold comprises the natural material.
16. The composition of claim 14 , wherein the scaffold comprises the synthetic material.
17. The composition of claim 10 , wherein the tanning comprises a vegetable tanning, a chrome tanning, an aldehyde tanning, a syntan tanning, a bacterial tanning, or any combination thereof.
Assignments (3)
ASSIGNMENT OF ASSIGNOR'S INTEREST
Recorded Jun 26, 2025
From: VITROLABS INC
To: FAIRCRAFT
Reel/Frame 071535/0661 →
ASSIGNMENT OF ASSIGNOR'S INTEREST
Recorded Aug 2, 2024
From: HELGASON, INGVAR
To: VITROLABS INC
Reel/Frame 068166/0470 →
ASSIGNMENT OF ASSIGNOR'S INTEREST
Recorded Aug 2, 2024
From: ILIC, DUSKO
To: KING'S COLLEGE LONDON
Reel/Frame 068294/0771 →
Continuity (9)
Provisional Application
62325819
· Apr 21, 2016
References Cited (104)
US 4485096A
· Bell
· 1984
[cited by applicant]
US 6497875B1
· Sorrell et al.
· 2002
[cited by applicant]
US 9428817B2
· Greene et al.
· 2016
[cited by applicant]
US 20010048917A1
· Hoeffler et al.
· 2001
[cited by applicant]
US 20060105454A1
· Son et al.
· 2006
[cited by applicant]
US 20070116676A1
· Kida
· 2007
[cited by examiner]
US 20070148138A1
· Barrows et al.
· 2007
[cited by applicant]
US 20090162896A1
· Scheibel
· 2009
[cited by applicant]
US 20100255059A1
· Marquez et al.
· 2010
[cited by applicant]
US 20110052693A1
· Kao et al.
· 2011
[cited by applicant]
US 20110165130A1
· Guenou
· 2011
[cited by applicant]
US 20130025003A1
· Trieu et al.
· 2013
[cited by applicant]
US 20130255003A1
· Forgacs
· 2013
[cited by examiner]
US 20140178346A1
· Byrne et al.
· 2014
[cited by applicant]
US 20150250925A1
· Akashi
· 2015
[cited by examiner]
US 20160097109A1
· Forgacs
· 2016
[cited by examiner]
US 20160097154A1
· Dumbrique et al.
· 2016
[cited by applicant]
US 20160348078A1
· Forgacs
· 2016
[cited by examiner]
US 20160354447A1
· Schmuck et al.
· 2016
[cited by applicant]
US 20190201579A1
· Tsuji et al.
· 2019
[cited by applicant]
CN 1468634A
· 2004
[cited by applicant]
CN 1528253A
· 2004
[cited by applicant]
CN 101264344A
· 2008
[cited by applicant]
CN 101330935A
· 2008
[cited by applicant]
CN 101538555A
· 2009
[cited by applicant]
CN 101792735A
· 2010
[cited by applicant]
CN 101802172A
· 2010
[cited by applicant]
CN 103893831A
· 2014
[cited by applicant]
CN 101385871B
· 2015
[cited by applicant]
EP 1334714A1
· 2003
[cited by applicant]
EP 1589098A1
· 2005
[cited by applicant]
EP 2103687A1
· 2009
[cited by applicant]
EP 2297299A1
· 2011
[cited by applicant]
EP 3473705A1
· 2019
[cited by applicant]
EP 3690029A1
· 2020
[cited by applicant]
EP 4067478A1
· 2022
[cited by applicant]
JP 2010100750A
· 2010
[cited by applicant]
JP 2016016267A
· 2016
[cited by applicant]
JP 6218238B2
· 2017
[cited by applicant]
KR 100648405B1
· 2006
[cited by applicant]
RU 2342164C2
· 2008
[cited by applicant]
RU 2568059C1
· 2015
[cited by applicant]
WO WO9945770A1
· 1999
[cited by applicant]
WO WO2008151058A2
· 2008
[cited by applicant]
WO WO2009156398A1
· 2009
[cited by applicant]
WO WO2014201406A1
· 2014
[cited by applicant]
WO WO2016073453A1
· 2016
[cited by applicant]
WO WO2017184967A1
· 2017
[cited by applicant]
Baxter. Welcome to the TISSEEL [Fibrin Sealant] Sealing Stops Bleeding. © 2017 Baxter Healthcare Corporation. 1 page. URL: http://www.tisseel.com/us/index.html.
[cited by applicant]
Bellas, et al. In vitro 3D full-thickness skin-equivalent tissue model using silk and collagen biomaterials. Macromol Biosci. Dec. 2012;12(12):1627-36. doi: 10.1002/mabi.201200262. Epub Nov. 19, 2012.
[cited by applicant]
Bilousova, et al. Differentiation of mouse induced pluripotent stem cells into a multipotent keratinocyte lineage. J Invest Dermatol. Apr. 2011;131(4):857-64.
[cited by applicant]
British Search and Examination Report dated Oct. 13, 2021 for United Kingdom Application Serial No. GB1706380.1.
[cited by applicant]
Buchholz, et al. Derivation of functional retinal pigmented epithelium from induced pluripotent stem cells. Stem Cells. Oct. 2009;27(10):2427-34.
[cited by applicant]
Burke, John F. et al. Successful use of a physiologically acceptable artificial skin in the treatment of extensive burn injury. Ann Surg 194(4):413-427 (1981).
[cited by applicant]
Bye, Frazer J. et al. Development of a Basement Membrane Substitute Incorporated Into an Electrospun Scaffold for 3D Skin Tissue Engineering. Journal of Biomaterials and Tissue Engineering 4(9):686-692 (2014).
[cited by applicant]
Carlson, et al. Three-dimensional tissue models of normal and diseased skin. Curr Protoc Cell Biol. Dec. 2008;Chapter 19:Unit 19.9. doi: 10.1002/0471143030.cb1909s41.
[cited by applicant]
CellnTec. Full Thickness Skin Models. CellnTec: Advanced Cell Systems. www.cellntec.com. Accessed Apr. 22, 2016. 3 pages.
[cited by applicant]
CellnTec. Proliferation/Differentiation. CellnTec: Advanced Cell Systems. Accessed Apr. 22, 2016. 3 pages. URL: http://cellntec.com/products/resources/protocols/culture/.
[cited by applicant]
Chen, et al., “Differetiation of Mouse Induced Pluripotent Stem Cells into a Multipotent Keratinocyte Lineage” Journal of Investigative Dermatology, 131(4), 857-864.
[cited by applicant]
Chen Y., et al. “Highly Rapid and Efficient Conversion of Human Fibroblasts to Keratinocyte-Like Cells”, Journal of Investigative Dermatology. 2014; 134(2):335-344; abstract.
[cited by applicant]
De Breij, et al. Three-dimensional human skin equivalent as a tool to study Acinetobacter baumannii colonization. Antimicrob Agents Chemother. May 2012;56(5):2459-64. doi: 10.1128/AAC.05975-11. Epub Jan. 30, 2012.
[cited by applicant]
Gledhill, et al. Melanin Transfer in Human 3D Skin Equivalents Generated Exclusively from Induced Pluripotent Stem Cells. Plos One. Published: Aug. 26, 2015. https://doi.org/10.1371/journal.pone.0136713.
[cited by applicant]
Hansbrough, John F. et al. Evaluation of Graftskin Composite Grafts on Full-Thickness Wounds on Athymic Mice. J Burn Care Rehabil 15:346-353 (1994).
[cited by applicant]
International Search Report and Written Opinion dated Sep. 22, 2017 for International PCT Patent Application No. PCT/US2017/028819.
[cited by applicant]
Itoh, et al. Generation of 3D skin equivalents fully reconstituted from human induced pluripotent stem cells (iPSCs). PLoS One. Oct. 11, 2013;8(10):e77673. doi: 10.1371/journal.pone.0077673. eCollection 2013.
[cited by applicant]
Kellouche, Sabrina. et al. Tissue engineering for full-thickness burns: A dermal substitute from bench to bedside. Biochem Biophys Res Commun 363(3):472-478 (2007).
[cited by applicant]
Kogut, et al. Differentiation of human induced pluripotent stem cells into a keratinocyte lineage. Methods Mol Biol. 2014; 1195:1-12. doi: 10.1007/7651_2013_64.
[cited by applicant]
Krugluger, et al. Reorganization of hair follicles in human skin organ culture induced by cultured human follicle-derived cells. Exp Dermatol. Aug. 2005;14(8):580-5.
[cited by applicant]
Petrova, Anastasia et al. 3D In vitro model of a functional epidermal permeability barrier from human embryonic stem cells and induced pluripotent stem cells. Stem Cell Reports 2(5):675-689 (2014).
[cited by applicant]
Quan T. et al. Enhancing structural support of the dermal microenvironment activates fibroblasts, endothelial cells, and keratinocytes in aged human skin in vivo. J Invest Dermatol. Mar. 2013;133(3):658-667. doi: 10.103…
[cited by applicant]
Randall, V A. et al. A comparison of the culture and growth of dermal papilla cells from hair follicles from non-balding and balding (androgenetic alopecia) scalp. British Journal of Dermatology 134:437-444 (1996).
[cited by applicant]
Rheinwald, James G, and Howard, Green. Serial Cultivation of Strains of Human Epidermal Keratinocytes: the Formation of Keratinizing Colonies from Single Cells. Cell 6:331-337 (1975).
[cited by applicant]
SearchReport dated Aug. 17, 2020 for Application No. 2018140838 (3 pages).
[cited by applicant]
Sekar et al., “A novel cross-linked human amniotic membrane for corneal implantations”, Nov. 15, 2012, Institution of Mechanical Engineers—Journal of Engineering Medicine, vol. 3, Issue 227, pp. 221-228 (Year:2012).
[cited by applicant]
Shamis Y., et al. “Fibroblasts Derived from Human Pluripotent Stem Cells Activate Angiogenic Responses In Vitro and In Vivo”, PLoS ONE.2013; 8(12): e83755; DOI: 10.1371/journal.pone.0083755; abstract.
[cited by applicant]
Souto, et al. Model for Human Skin Reconstructed in Vitro Composed of Associated Dermis and Epidermis. Sao Paulo Med J 124 (2), 71-76. Mar. 2, 2006.
[cited by applicant]
Stratatech. Core Technology. © 2017 Stratatech a Mallinckrodt Company. 2 pages. URL: http://www.stratatechcorp.com/science/index.php.
[cited by applicant]
Sun, Richard. et al. Lowered Humidity Produces Human Epidermal Equivalents with Enhanced Barrier Properties. Tissue Eng Part C Methods 21(1):15-22 (2015).
[cited by applicant]
Supplementary Partial European Search Report dated Nov. 6, 2019 for Application Serial No. EP 17 78 6708, (11 pages).
[cited by applicant]
Takahashi, Kazutoshi. et al. Induction of pluripotent stem cells from adult human fibroblasts by defined factors. Cell 131(5):861-872 (2007).
[cited by applicant]
U.S. Appl. No. 16/095,308 Final Office Action dated Oct. 30, 2020.
[cited by applicant]
U.S. Appl. No. 16/095,308 Non-Final Office Action dated Aug. 8, 2019.
[cited by applicant]
U.S. Appl. No. 16/299,734 Non-Final Office Action dated Sep. 17, 2019.
[cited by applicant]
U.S. Appl. No. 16/892,839 Non-Final Office Action dated Feb. 12, 2021.
[cited by applicant]
Varani J. Preservation of human skin structure and function in organ culture. Histol Histopathol. Jul. 1998;13(3):775-83. doi: 10.14670/HH-13.775. Abstract.
[cited by applicant]
Verkhovskaya L.Z. et al., The effect of glycerol alkoxy-derivatives on morphological and functional properties of continuous cell culture, Cryobiology, 1990.
[cited by applicant]
Wang Y., et al. “Biomimetic fibroblast-loaded artificial dermis with “sandwich” structure and designed gradient pore sizes promotes wound healing by favoring granulation tissue formation and wound re-epithelialization”,…
[cited by applicant]
Xie L., et al. “CD10 expressed by fibroblasts and melanoma cells degrades endothelin-1 secreted by human keratinocytes”, Eur JDermatol. 2011;21(4):505-509; DOI: 10.1684/ejd.2011.1371; abstract.
[cited by applicant]
Yang, et al. Generation of folliculogenic human epithelial stem cells from induced pluripotent stem cells. Nat Commun. 2014; 5: 3071. Author manuscript; available in PMC Jul. 1, 2014.
[cited by applicant]
Yu, Junying. et al. Induced pluripotent stem cell lines derived from human somatic cells. Science 318(5858):1917-1920 (2007).
[cited by applicant]
Michel, Martine, et al. Characterization of a new tissue-engineered human skin equivalent with hair. In Vitro Cellular & Developmental Biology—Animal 35: 318-326. (1999).
[cited by applicant]