IP Library › Granted Patent US 12,352,679
Granted Patent B2
US 12,352,679 · App. 19/026,954 · Granted Jul 8, 2025

Synthetic human cell mimic particle for cytometric or coulter device

Inventors: Jeffrey Kim (Berkeley, CA); Oliver Liu (San Francisco, CA); Jeremy Agresti (El Cerrito, CA); Anh Tuan Nguyen (San Francisco, CA)
Assignee: Slingshot Biosciences, Inc.
G01N15/1012C12Q1/04G01N1/28G01N15/1434G01N15/1459G01N2001/2893G01N15/01G01N2015/1006G01N2015/1014G01N15/149
View Patent ↗
Loading inventors, assignments & file history…
Monitor This Case
Get email alerts when status or documents change.
Order Certified Copies
Most orders are placed with the USPTO same day — all within 24 business hours.
Order via The Patent Place →
Pre-filled with this patent's details
Quick Facts
Patent No.
US 12,352,679
App. No.
19/026,954
Filed
Jan 17, 2025
Granted
Jul 8, 2025
Kind
B2
Examiner
XU, XIAOYUN
Art Unit
1797
USPC
435/34
Abstract

Synthetic human cell mimic hydrogel particles and their use in cytometric or coulter device applications are described. The synthetic human cell mimic hydrogel particles described herein are selectively tunable to have at least one optical, volumetric, or capacitance property that is substantially similar to a corresponding optical, volumetric, or capacitance property of the human cell mimic hydrogel particle's natural biological cell counterpart.

Claims (20)

1. A hydrogel particle comprising a polymer matrix attached to a plurality of different biomolecules comprising CD3 and/or CD28.

2. The hydrogel particle of claim 1 , wherein the plurality of different biomolecules are attached to the polymer matrix via one or more functional groups.

3. The hydrogel particle of claim 1 , wherein the plurality of different biomolecules are attached to the polymer matrix via a linker.

4. The hydrogel particle of claim 1 , wherein the plurality of different biomolecules further comprise CD4, CD8, CD25, CD69, CD19, CD20, CD40, CD16, CD56, or any combinations thereof.

5. The hydrogel particle of claim 1 , wherein the plurality of different biomolecules comprise CD3 and CD28.

6. The hydrogel particle of claim 5 , wherein the plurality of different biomolecules are not attached to an immune cell.

7. The hydrogel particle of claim 6 , wherein the immune cell is selected from a B cell, a T cell, a dendritic cell, a natural killer cell, a macrophage, a monocyte, and a neutrophil.

8. The hydrogel particle of claim 6 , wherein the immune cell is a T cell.

9. The hydrogel particle of claim 8 , wherein the T cell is an activated T cell.

10. The hydrogel particle of claim 1 , wherein in an aqueous solution the hydrogel particle exhibits an optical-scatter property that is substantially similar to a corresponding optical-scatter property of a natural biological cell counterpart.

11. The hydrogel particle of claim 10 , wherein the optical-scatter property that is substantially similar to the corresponding optical-scatter property of the natural biological cell counterpart is side scatter (SSC).

12. The hydrogel particle of claim 10 , wherein the optical-scatter property that is substantially similar to the corresponding optical-scatter property of the natural biological cell counterpart is forward scatter (FSC).

13. The hydrogel particle of claim 1 , wherein the hydrogel particle comprises a diameter ranging from 1-100 μm.

14. The hydrogel particle of claim 1 , wherein the hydrogel polymer matrix is synthesized by polymerizing a monomer.

15. The hydrogel particle of claim 1 , wherein the polymer matrix comprises a polymerized monomer selected from hydroxyethyl methacrylate, ethyl methacrylate, 2-hydroxyethyl methacrylate (HEMA), propylene glycol methacrylate, acrylamide, N-vinylpyrrolidone (NVP), methyl methacrylate, glycidyl methacrylate, glycerol methacrylate (GMA), glycol methacrylate, ethylene glycol, fumaric acid, 2-hydroxyethyl methacrylate, hydroxyethoxyethyl methacrylate, hydroxydiethoxyethyl methacrylate, methoxyethyl methacrylate, methoxyethoxyethyl methacrylate, methoxydiethoxyethyl methacrylate, poly(ethylene glycol) methacrylate, methoxy-poly(ethylene glycol) methacrylate, methacrylic acid, sodium methacrylate, glycerol methacrylate, hydroxypropyl methacrylate, hydroxybutyl methacrylate, phenyl acrylate, phenyl methacrylate, benzyl acrylate, benzyl methacrylate, 2-phenylethyl acrylate, 2-phenylethyl methacrylate, 2-phenoxyethyl acrylate, 2-phenoxyethyl methacrylate, phenylthioethyl acrylate, phenylthioethyl methacrylate, 2,4,6-tribromophenyl acrylate, 2,4,6-tribromophenyl methacrylate, pentabromophenyl acrylate, pentabromophenyl methacrylate, pentachlorophenyl acrylate, pentachlorophenyl methacrylate, 2,3-dibromopropyl acrylate, 2,3-dibromopropyl methacrylate, 2-naphthyl acrylate, 2-naphthyl methacrylate, 4-methoxybenzyl acrylate, 4-methoxybenzyl methacrylate, 2-benzyloxyethyl acrylate, 2-benzyloxyethyl methacrylate, 4-chlorophenoxyethyl acrylate, 4-chlorophenoxyethyl methacrylate, 2-phenoxyethoxyethyl acrylate, 2-phenoxyethoxyethyl methacrylate, N-phenyl acrylamide, N-phenyl methacrylamide, N-benzyl acrylamide, N-benzyl methacrylamide, N,N-dibenzyl acrylamide, N,N-dibenzyl methacrylamide, N-diphenylmethyl acrylamide N-(4-methylphenyl)methyl acrylamide, N-1-naphthyl acrylamide, N-4-nitrophenyl acrylamide, N-(2-phenylethyl)acrylamide, N-triphenylmethyl acrylamide, N-(4-hydroxyphenyl)acrylamide, N,N-methylphenyl acrylamide, N,N-phenyl phenylethyl acrylamide, N-diphenylmethyl methacrylamide, N-(4-methyl phenyl)methyl methacrylamide, N-1-naphthyl methacrylamide, N-4-nitrophenyl methacrylamide, N-(2-phenylethyl)methacrylamide, N-triphenylmethyl methacrylamide, N-(4-hydroxyphenyl)methacrylamide, N,N-methylphenyl methacrylamide, N,N′-phenyl phenylethyl methacrylamide, N-vinylcarbazole, 4-vinylpyridine, 2-vinylpyridine, and a combination thereof.

16. The hydrogel particle of claim 1 , wherein the polymer matrix comprises a polymerized biodegradable monomer.

17. The hydrogel particle of claim 16 , wherein the biodegradable monomer is a monosaccharide, disaccharide, polysaccharide, peptide, protein, or protein domain.

18. The hydrogel particle of claim 16 , wherein the biodegradable monomer is selected from the group consisting of agar, agarose, alginic acid, alguronic acid, alpha glucan, amylopectin, amylose, arabinoxylan, beta-glucan, callose, capsulan, carrageenan polysaccharide, cellodextrin, cellulin, cellulose, chitin, chitosan, chrysolaminarin, curdlan, cyclodextrin, alpha-cyclodextrin, dextrin, dextran, ficoll, fructan, fucoidan, galactoglucomannan, galactomannan, galactosaminogalactan, gellan gum, glucan, glucomannan, glucuronoxylan, glycocalyx, glycogen, hemicellulose, homopolysaccharide, hypromellose, icodextrin, inulin, kefiran, laminarin, lentinan, levan polysaccharide, lichenin, mannan, mixed-linkage gluxan, paramylon, pectic acid, pectin, pentastarch, phytoglycogen, pleuran, polydextrose, polysaccharide peptide, porphyran, pullulan, schizophyllan, sinistrin, sizofiran, welan gum, xanthan gum, xylan, xyloglucan, zymosan, and a combination thereof.

19. The hydrogel particle of claim 1 , wherein the polymer matrix comprises a bifunctional monomer.

20. A population of the hydrogel particles of claim 1 .

Assignments (1)
ASSIGNMENT OF ASSIGNOR'S INTEREST Recorded Jan 21, 2025
From: KIM, JEFFREY; NGUYEN, ANH TUAN; LIU, OLIVER; AGRESTI, JEREMY
To: SLINGSHOT BIOSCIENCES, INC.
Reel/Frame 069935/0129 →
Continuity (10)
Continuation 18767094 · Jul 9, 2024
Continuation 18417986 · Jan 19, 2024
Continuation 18139741 · Apr 26, 2023
Continuation 17990360 · Nov 18, 2022
Continuation 16933028 · Jul 20, 2020
Continuation 15625394 · Jun 16, 2017
Continuation 15018769 · Feb 8, 2016
Provisional Application 62184192 · Jun 24, 2015
Provisional Application 62114004 · Feb 9, 2015
Related Publication 20250164373A1 · May 22, 2025
References Cited (387)
US 3822095A · Hirschfeld · 1974 [cited by applicant]
US 3872312A · Hirschfeld · 1975 [cited by applicant]
US 3916205A · Kleinerman · 1975 [cited by applicant]
US 3937799A · Lewin et al. · 1976 [cited by applicant]
US 3947564A · Shannon et al. · 1976 [cited by applicant]
US 3975084A · Block · 1976 [cited by applicant]
US 4185964A · Lancaster · 1980 [cited by applicant]
US 4271123A · Curry et al. · 1981 [cited by applicant]
US 4295199A · Curry et al. · 1981 [cited by applicant]
US 4389491A · Hanamoto et al. · 1983 [cited by applicant]
US 4409335A · Hanamoto et al. · 1983 [cited by applicant]
US 4448888A · Bleile et al. · 1984 [cited by applicant]
US 4511662A · Baran et al. · 1985 [cited by applicant]
US 4704891A · Recktenwald et al. · 1987 [cited by applicant]
US 4774189A · Schwartz · 1988 [cited by applicant]
US 4857451A · Schwartz · 1989 [cited by applicant]
US 5073498A · Schwartz et al. · 1991 [cited by applicant]
US 5093234A · Schwartz · 1992 [cited by applicant]
US 5244799A · Anderson · 1993 [cited by applicant]
US 5283079A · Wang et al. · 1994 [cited by applicant]
US 5395688A · Wang et al. · 1995 [cited by applicant]
US 5820879A · Fernandez et al. · 1998 [cited by applicant]
US 5841139A · Sostek et al. · 1998 [cited by applicant]
US 5871722A · Nacht et al. · 1999 [cited by applicant]
US 5888823A · Matsumoto et al. · 1999 [cited by applicant]
US 6043506A · Heffelfinger et al. · 2000 [cited by applicant]
US 6107365A · Bertozzi et al. · 2000 [cited by applicant]
US 6108082A · Pettipiece et al. · 2000 [cited by applicant]
US 6214539B1 · Cosand · 2001 [cited by applicant]
US 6280618B2 · Watkins et al. · 2001 [cited by applicant]
US 6372813B1 · Johnson et al. · 2002 [cited by applicant]
US 6516537B1 · Teich et al. · 2003 [cited by applicant]
US 6586176B1 · Trnovsky et al. · 2003 [cited by applicant]
US 6657030B2 · Vanderbilt · 2003 [cited by applicant]
US 6762055B2 · Carver et al. · 2004 [cited by applicant]
US 6806058B2 · Jesperson et al. · 2004 [cited by applicant]
US 6872578B2 · Watkins et al. · 2005 [cited by applicant]
US 6897072B1 · Rich et al. · 2005 [cited by applicant]
US 6897954B2 · Bishop et al. · 2005 [cited by applicant]
US 7045366B2 · Huang et al. · 2006 [cited by applicant]
US RE39542E · Jain et al. · 2007 [cited by applicant]
US 7205156B2 · Rich et al. · 2007 [cited by applicant]
US 7294503B2 · Quake et al. · 2007 [cited by applicant]
US 7314584B2 · Tsutsui et al. · 2008 [cited by applicant]
US 7465538B2 · Watkins et al. · 2008 [cited by applicant]
US 7479631B2 · Rich et al. · 2009 [cited by applicant]
US 7482161B2 · Carver et al. · 2009 [cited by applicant]
US 7482167B2 · Sammak et al. · 2009 [cited by applicant]
US 7531357B2 · Carver et al. · 2009 [cited by applicant]
US 7569399B2 · Watkins et al. · 2009 [cited by applicant]
US 7588942B2 · Ho et al. · 2009 [cited by applicant]
US 7601539B2 · Kawate · 2009 [cited by applicant]
US 7776927B2 · Chu et al. · 2010 [cited by applicant]
US 7842498B2 · Um et al. · 2010 [cited by applicant]
US 8030095B2 · Harriman · 2011 [cited by applicant]
US 8105845B2 · Notcovich et al. · 2012 [cited by applicant]
US 8114580B2 · Carver et al. · 2012 [cited by applicant]
US 8187885B2 · Purvis, Jr. · 2012 [cited by applicant]
US 8415161B2 · Yan et al. · 2013 [cited by applicant]
US 8415173B2 · Harriman · 2013 [cited by applicant]
US 8451450B2 · Heng · 2013 [cited by applicant]
US 8580530B2 · Buffiere et al. · 2013 [cited by applicant]
US 8580531B2 · Buffiere et al. · 2013 [cited by applicant]
US 8603828B2 · Walker et al. · 2013 [cited by applicant]
US 8609363B2 · Heng et al. · 2013 [cited by applicant]
US 8704158B2 · Haberstroh et al. · 2014 [cited by applicant]
US 8748183B2 · Durack et al. · 2014 [cited by applicant]
US 9012167B2 · Dallenne et al. · 2015 [cited by applicant]
US 9110050B2 · Likuski et al. · 2015 [cited by applicant]
US 9175421B2 · Notcovich et al. · 2015 [cited by applicant]
US 9176154B2 · Darmstadt et al. · 2015 [cited by applicant]
US 9213034B2 · Walker et al. · 2015 [cited by applicant]
US 9217175B2 · Regan et al. · 2015 [cited by applicant]
US 9228898B2 · Kiani et al. · 2016 [cited by applicant]
US 9417190B2 · Hindson et al. · 2016 [cited by applicant]
US 9476101B2 · Pregibon et al. · 2016 [cited by applicant]
US 9658220B2 · King et al. · 2017 [cited by applicant]
US 9696257B2 · Fox et al. · 2017 [cited by applicant]
US 9714897B2 · Kim et al. · 2017 [cited by applicant]
US 9804149B2 · Darmstadt et al. · 2017 [cited by applicant]
US 9816931B2 · Abate et al. · 2017 [cited by applicant]
US 9915598B2 · Kim et al. · 2018 [cited by applicant]
US 10067135B2 · Kaul et al. · 2018 [cited by applicant]
US 10180385B2 · Fox et al. · 2019 [cited by applicant]
US 10191039B2 · King et al. · 2019 [cited by applicant]
US 10328160B2 · Trogler et al. · 2019 [cited by applicant]
US 10343167B2 · Esmail et al. · 2019 [cited by applicant]
US 10344100B1 · Vashist et al. · 2019 [cited by applicant]
US 10392557B2 · Chan · 2019 [cited by applicant]
US 10416070B1 · Handique · 2019 [cited by applicant]
US 10429291B2 · Fox et al. · 2019 [cited by applicant]
US 10481068B2 · Kim et al. · 2019 [cited by applicant]
US 10494607B2 · Edinger et al. · 2019 [cited by applicant]
US 10508990B2 · Fox et al. · 2019 [cited by applicant]
US 10732189B2 · Buffiere et al. · 2020 [cited by applicant]
US 10753846B2 · Kim et al. · 2020 [cited by applicant]
US 10942109B2 · Kim et al. · 2021 [cited by applicant]
US 11047845B1 · Barry, Jr. et al. · 2021 [cited by applicant]
US 11085036B2 · Norberg et al. · 2021 [cited by applicant]
US 11118217B2 · Xue et al. · 2021 [cited by applicant]
US 11155809B2 · Lebofsky · 2021 [cited by applicant]
US 11180752B2 · Wu et al. · 2021 [cited by applicant]
US 11186862B2 · Lebofsky et al. · 2021 [cited by applicant]
US 11213490B2 · Shoichet et al. · 2022 [cited by applicant]
US 11231355B2 · Handique · 2022 [cited by applicant]
US 11274337B2 · Xue et al. · 2022 [cited by applicant]
US 11300496B2 · Handique · 2022 [cited by applicant]
US 11313782B2 · Kim et al. · 2022 [cited by applicant]
US 11479816B2 · Lebofsky et al. · 2022 [cited by applicant]
US 11506655B2 · Hunsley et al. · 2022 [cited by applicant]
US 11598768B2 · Kim · 2023 [cited by applicant]
US 11603556B2 · Lebofsky · 2023 [cited by applicant]
US 11663717B2 · Barnes et al. · 2023 [cited by applicant]
US 11686661B2 · Kim et al. · 2023 [cited by applicant]
US 11726023B2 · Kim et al. · 2023 [cited by applicant]
US 11747261B2 · Kim et al. · 2023 [cited by applicant]
US 11761877B2 · Kim et al. · 2023 [cited by applicant]
US 11927519B2 · Kim et al. · 2024 [cited by applicant]
US 12038369B2 · Kim et al. · 2024 [cited by applicant]
US 12066369B2 · Kim et al. · 2024 [cited by applicant]
US 12130285B2 · Ahn et al. · 2024 [cited by applicant]
US 12134779B2 · Nguyen et al. · 2024 [cited by applicant]
US 12196661B2 · Zhang et al. · 2025 [cited by applicant]
US 12228492B2 · Kim et al. · 2025 [cited by applicant]
US 20010008217A1 · Watkins et al. · 2001 [cited by applicant]
US 20010054580A1 · Watkins et al. · 2001 [cited by applicant]
US 20020058019A1 · Berenson et al. · 2002 [cited by applicant]
US 20020106730A1 · Coyle et al. · 2002 [cited by applicant]
US 20020115116A1 · Song et al. · 2002 [cited by applicant]
US 20030013116A1 · Song et al. · 2003 [cited by applicant]
US 20030022157A1 · Zauderer et al. · 2003 [cited by applicant]
US 20030064403A1 · Song et al. · 2003 [cited by applicant]
US 20030124371A1 · Um et al. · 2003 [cited by applicant]
US 20030132538A1 · Chandler · 2003 [cited by applicant]
US 20030190749A1 · Surber et al. · 2003 [cited by applicant]
US 20030198995A1 · Sabbadini et al. · 2003 [cited by applicant]
US 20030218130A1 · Boschetti et al. · 2003 [cited by applicant]
US 20030224444A1 · Sabbadini et al. · 2003 [cited by applicant]
US 20030232323A1 · Freeman et al. · 2003 [cited by applicant]
US 20040126904A1 · Watkins et al. · 2004 [cited by applicant]
US 20040137577A1 · Coyle et al. · 2004 [cited by applicant]
US 20040151704A1 · Berenson et al. · 2004 [cited by applicant]
US 20050059086A1 · Huang et al. · 2005 [cited by applicant]
US 20050090016A1 · Rich et al. · 2005 [cited by applicant]
US 20050112650A1 · Chang et al. · 2005 [cited by applicant]
US 20050118230A1 · Hill et al. · 2005 [cited by applicant]
US 20050172476A1 · Stone et al. · 2005 [cited by applicant]
US 20050176056A1 · Sammak et al. · 2005 [cited by applicant]
US 20050208573A1 · Bell et al. · 2005 [cited by applicant]
US 20060163385A1 · Link et al. · 2006 [cited by applicant]
US 20060223187A1 · Carver et al. · 2006 [cited by applicant]
US 20060240560A1 · Bakker et al. · 2006 [cited by applicant]
US 20060250616A1 · Pettipiece et al. · 2006 [cited by applicant]
US 20060269962A1 · Watkins et al. · 2006 [cited by applicant]
US 20060275820A1 · Watkins et al. · 2006 [cited by applicant]
US 20070000342A1 · Kazuno · 2007 [cited by applicant]
US 20070003442A1 · Link et al. · 2007 [cited by applicant]
US 20070054119A1 · Garstecki et al. · 2007 [cited by applicant]
US 20070082019A1 · Huang et al. · 2007 [cited by applicant]
US 20070087348A1 · Notcovich et al. · 2007 [cited by applicant]
US 20070118297A1 · Thayer · 2007 [cited by applicant]
US 20070158547A1 · Rich et al. · 2007 [cited by applicant]
US 20070178168A1 · Ho et al. · 2007 [cited by applicant]
US 20070254378A1 · Zhang et al. · 2007 [cited by applicant]
US 20070259415A1 · Zigova et al. · 2007 [cited by applicant]
US 20080014589A1 · Link et al. · 2008 [cited by applicant]
US 20080019921A1 · Zhang · 2008 [cited by applicant]
US 20080023630A1 · Boschetti et al. · 2008 [cited by applicant]
US 20080026468A1 · Carver et al. · 2008 [cited by applicant]
US 20080032405A1 · Ho et al. · 2008 [cited by applicant]
US 20080044472A1 · Garcia et al. · 2008 [cited by applicant]
US 20080090737A1 · Boschetti · 2008 [cited by applicant]
US 20080241262A1 · Lee et al. · 2008 [cited by applicant]
US 20090028945A1 · Cheung et al. · 2009 [cited by applicant]
US 20090148961A1 · Luchini et al. · 2009 [cited by applicant]
US 20100029794A1 · Yilmaz et al. · 2010 [cited by applicant]
US 20100120059A1 · Yan et al. · 2010 [cited by applicant]
US 20100178647A1 · Carver et al. · 2010 [cited by applicant]
US 20100178656A1 · Buffiere et al. · 2010 [cited by applicant]
US 20100184101A1 · Buffiere et al. · 2010 [cited by applicant]
US 20100187441A1 · Waldbeser et al. · 2010 [cited by applicant]
US 20100234252A1 · Moradi-Araghi et al. · 2010 [cited by applicant]
US 20100285594A1 · Purvis, Jr. · 2010 [cited by applicant]
US 20100303811A1 · Ochi · 2010 [cited by applicant]
US 20110022164A1 · Quinn et al. · 2011 [cited by applicant]
US 20110117670A1 · Walker et al. · 2011 [cited by applicant]
US 20110212179A1 · Liu · 2011 [cited by applicant]
US 20110218123A1 · Weitz et al. · 2011 [cited by applicant]
US 20110222068A1 · Heng · 2011 [cited by applicant]
US 20110300629A1 · Hawkins et al. · 2011 [cited by applicant]
US 20110318820A1 · Hinz et al. · 2011 [cited by applicant]
US 20120065614A1 · Omary et al. · 2012 [cited by applicant]
US 20120129723A1 · Notcovich et al. · 2012 [cited by applicant]
US 20120295300A1 · Heng et al. · 2012 [cited by applicant]
US 20120309651A1 · Pregibon et al. · 2012 [cited by applicant]
US 20130089883A1 · Dallenne et al. · 2013 [cited by applicant]
US 20130173618A1 · Banville et al. · 2013 [cited by applicant]
US 20130177973A1 · Kondo · 2013 [cited by applicant]
US 20130274125A1 · Binder et al. · 2013 [cited by applicant]
US 20140073532A1 · Walker et al. · 2014 [cited by applicant]
US 20140100791A1 · Darmstadt et al. · 2014 [cited by applicant]
US 20140142039A1 · Little et al. · 2014 [cited by applicant]
US 20140157859A1 · Darmstadt et al. · 2014 [cited by applicant]
US 20140179808A1 · Flanagan · 2014 [cited by applicant]
US 20140198313A1 · Tracy et al. · 2014 [cited by applicant]
US 20140221238A1 · Regan et al. · 2014 [cited by applicant]
US 20140271677A1 · Palese et al. · 2014 [cited by applicant]
US 20140377334A1 · Irvine et al. · 2014 [cited by applicant]
US 20150027207A1 · Likuski et al. · 2015 [cited by applicant]
US 20150094232A1 · Abate et al. · 2015 [cited by applicant]
US 20150177115A1 · Kim et al. · 2015 [cited by applicant]
US 20150211044A1 · Dallenne et al. · 2015 [cited by applicant]
US 20150267196A1 · Alsberg et al. · 2015 [cited by applicant]
US 20150362499A1 · Chan · 2015 [cited by applicant]
US 20160258856A1 · Kim et al. · 2016 [cited by applicant]
US 20160299051A1 · Kim et al. · 2016 [cited by applicant]
US 20170042995A1 · Ali et al. · 2017 [cited by applicant]
US 20170045436A1 · Fox et al. · 2017 [cited by applicant]
US 20170081636A1 · Kevlahan et al. · 2017 [cited by applicant]
US 20170138856A1 · Li et al. · 2017 [cited by applicant]
US 20170159132A1 · Okino et al. · 2017 [cited by applicant]
US 20170268998A1 · Fox et al. · 2017 [cited by applicant]
US 20170361322A1 · Esmail et al. · 2017 [cited by applicant]
US 20170370951A1 · Buffiere et al. · 2017 [cited by applicant]
US 20180088112A1 · Fan et al. · 2018 [cited by applicant]
US 20180172687A1 · Kaul et al. · 2018 [cited by applicant]
US 20180216171A1 · Xue et al. · 2018 [cited by applicant]
US 20180247195A1 · Kumar et al. · 2018 [cited by applicant]
US 20180275040A1 · Kim et al. · 2018 [cited by applicant]
US 20180282423A1 · Wang et al. · 2018 [cited by applicant]
US 20180371525A1 · Lebofsky et al. · 2018 [cited by applicant]
US 20190145881A1 · Fox et al. · 2019 [cited by applicant]
US 20190154707A1 · Flamini et al. · 2019 [cited by applicant]
US 20190169572A1 · Shi et al. · 2019 [cited by applicant]
US 20190249171A1 · Wu et al. · 2019 [cited by applicant]
US 20190293546A1 · Handique · 2019 [cited by applicant]
US 20200056231A1 · Lebofsky et al. · 2020 [cited by applicant]
US 20200085971A1 · Kevlahan et al. · 2020 [cited by applicant]
US 20200115675A1 · Pathak et al. · 2020 [cited by applicant]
US 20200150020A1 · Kim et al. · 2020 [cited by applicant]
US 20200206145A1 · Shi et al. · 2020 [cited by applicant]
US 20200209064A1 · Owsley et al. · 2020 [cited by applicant]
US 20200230159A1 · Ma et al. · 2020 [cited by applicant]
US 20200232979A1 · Revzin et al. · 2020 [cited by applicant]
US 20200249242A1 · Batxelli-Molina et al. · 2020 [cited by applicant]
US 20200268845A1 · Peled et al. · 2020 [cited by applicant]
US 20200332354A1 · Xue et al. · 2020 [cited by applicant]
US 20200363434A1 · Buffiere et al. · 2020 [cited by applicant]
US 20200399428A1 · Kleine-Brüggeney et al. · 2020 [cited by applicant]
US 20200400546A1 · Kim et al. · 2020 [cited by applicant]
US 20200408747A1 · Zur Megede et al. · 2020 [cited by applicant]
US 20210032297A1 · Schmidt et al. · 2021 [cited by applicant]
US 20210040567A1 · Handique et al. · 2021 [cited by applicant]
US 20210130880A1 · Lebofsky · 2021 [cited by applicant]
US 20210190740A1 · Scolari et al. · 2021 [cited by applicant]
US 20210231552A1 · Kim et al. · 2021 [cited by applicant]
US 20210236554A1 · Sadelain et al. · 2021 [cited by applicant]
US 20210247294A1 · Handique · 2021 [cited by applicant]
US 20210341469A1 · Kim et al. · 2021 [cited by applicant]
US 20220042077A1 · Lebofsky et al. · 2022 [cited by applicant]
US 20220065878A1 · Lee · 2022 [cited by applicant]
US 20220143160A1 · Miller et al. · 2022 [cited by applicant]
US 20220152150A1 · Koshy et al. · 2022 [cited by applicant]
US 20220154266A1 · Xue et al. · 2022 [cited by applicant]
US 20220178810A1 · Kim et al. · 2022 [cited by applicant]
US 20220213530A1 · Larson et al. · 2022 [cited by applicant]
US 20220260476A1 · Kim et al. · 2022 [cited by applicant]
US 20220364976A1 · Kim et al. · 2022 [cited by applicant]
US 20230012786A1 · Lebofsky et al. · 2023 [cited by applicant]
US 20230047416A1 · Umberger et al. · 2023 [cited by applicant]
US 20230062518A1 · Ebrahim et al. · 2023 [cited by applicant]
US 20230067460A1 · Nguyen et al. · 2023 [cited by applicant]
US 20230152202A1 · Kim et al. · 2023 [cited by applicant]
US 20230176042A1 · Kim et al. · 2023 [cited by applicant]
US 20230266223A1 · Kim et al. · 2023 [cited by applicant]
US 20240053248A1 · Kim et al. · 2024 [cited by applicant]
US 20240060038A1 · Nguyen et al. · 2024 [cited by applicant]
US 20240159645A1 · Kim et al. · 2024 [cited by applicant]
US 20240219382A1 · Ahn et al. · 2024 [cited by applicant]
US 20240269185A1 · Nguyen et al. · 2024 [cited by applicant]
US 20240319067A1 · Kim et al. · 2024 [cited by applicant]
US 20240353305A1 · Zhang et al. · 2024 [cited by applicant]
US 20250011712A1 · Nguyen et al. · 2025 [cited by applicant]
US 20250052772A1 · Zhang et al. · 2025 [cited by applicant]
US 20250076175A1 · Kim et al. · 2025 [cited by applicant]
CN 1158997A · 1997 [cited by applicant]
CN 101214217A · 2008 [cited by applicant]
CN 101245368A · 2008 [cited by applicant]
CN 102438740A · 2012 [cited by applicant]
CN 102539397A · 2012 [cited by applicant]
CN 102604305A · 2012 [cited by applicant]
CN 102675501A · 2012 [cited by applicant]
CN 103718044A · 2014 [cited by applicant]
CN 103744185A · 2014 [cited by applicant]
CN 104641217A · 2015 [cited by applicant]
DE 102018115391A1 · 2020 [cited by applicant]
EP 2576839B1 · 2017 [cited by applicant]
EP 3585364A1 · 2020 [cited by applicant]
JP H07196916A · 1995 [cited by applicant]
JP 2002510541A · 2002 [cited by applicant]
JP 2005281470A · 2005 [cited by applicant]
JP 2007114026A · 2007 [cited by applicant]
JP 2010265291A · 2010 [cited by applicant]
JP 2012011269A · 2012 [cited by applicant]
JP 2013520530A · 2013 [cited by applicant]
JP 2013155358A · 2013 [cited by applicant]
JP 2014058557A · 2014 [cited by applicant]
JP 2014508516A · 2014 [cited by applicant]
JP 2015530361A · 2015 [cited by applicant]
JP 2017507191A · 2017 [cited by applicant]
WO WO8910566A1 · 1989 [cited by applicant]
WO WO1995003408A1 · 1995 [cited by applicant]
WO WO0008212A1 · 2000 [cited by applicant]
WO WO0132829A2 · 2001 [cited by applicant]
WO WO03000014A2 · 2003 [cited by applicant]
WO WO2005013896A2 · 2005 [cited by applicant]
WO WO2006003423A2 · 2006 [cited by applicant]
WO WO2006078841A1 · 2006 [cited by applicant]
WO WO2006096571A2 · 2006 [cited by applicant]
WO WO2007101130A2 · 2007 [cited by applicant]
WO WO2008115653A2 · 2008 [cited by applicant]
WO WO2008121342A2 · 2008 [cited by applicant]
WO WO2010025190A1 · 2010 [cited by applicant]
WO WO2010025988A1 · 2010 [cited by applicant]
WO WO2011098407A1 · 2011 [cited by applicant]
WO WO2012033811A1 · 2012 [cited by applicant]
WO WO2013113670A1 · 2013 [cited by applicant]
WO WO2014089009A1 · 2014 [cited by applicant]
WO WO2014110591A1 · 2014 [cited by applicant]
WO WO2014209855A1 · 2014 [cited by applicant]
WO WO2016130489A1 · 2016 [cited by applicant]
WO WO2018108341A1 · 2018 [cited by applicant]
WO WO2019018727A1 · 2019 [cited by applicant]
WO WO2020037214A1 · 2020 [cited by applicant]
WO WO2021113065A1 · 2021 [cited by applicant]
WO WO2021154900A1 · 2021 [cited by applicant]
WO WO2023215886A1 · 2023 [cited by applicant]
WO WO2024092161A2 · 2024 [cited by applicant]
Bagwell et al., Fluorescence spectral overlap compensation for any No. of flow cytometry parameters, Ann NY Acad Sci. 677:167-184 (Mar. 1993). [cited by applicant]
Bigos et al., “Nine Color Eleven Parameter Immunophenotyping Using Three Laser Flow Cytometry,” Cytometry 36: 36-45 (1999). [cited by applicant]
Kim, J.S., et al., “Utilization of Microparticles in Next-generation Assays for Microflow Cytometers,” Analytical and Bioanalytical Chemistry, Nov. 2010, vol. 398 (6), pp. 2373-2382. [cited by applicant]
Roederer et al., “Eight Color, 10-Parameter F1mv Cytometry to Elucidate Complex Leukocyte Heterogeneity”, Cytometry 29:328-339 (1997). [cited by applicant]
Atkin-Smith et al., “Isolation of cell type-specific apoptotic bodies by fluorescence-activated cell sorting,” Scientific Reports, vol. 7, No. 1, Feb. 1, 2017, pp. 1-7. [cited by applicant]
Bele, Marjan, Olavi Siiman and Egon Matjevic, “Preparation and flow cytometry of uniform silica-fluorescent dye microspheres.” Journal of colloid and interface science 254(2):274-282 (2002). [cited by applicant]
Chen et al., “Molecular mechanisms of T cell co-stimulation and co-inhibition,” Nature reviews Immunology, 13:227-242 (2013). [cited by applicant]
Chen, M., et al., “Initiator caspases in apoptosis signaling pathways”, Apoptosis (London), Aug. 1, 2002, pp. 313-319, DOI: 10.1023/A:1016167228059. [cited by applicant]
Claus et al., “The emerging landscape of novel 4-1BB (CD137) agonistic drugs for cancer immunotherapy,” MAbs 15(1):2167189, pp. 1-22 (Jan.-Dec. 2023). [cited by applicant]
Elbert, “Liquid-liquid two-phase systems for the production of porous hydrogels and hydrogel microspheres for biomedical applications: A tutorial review,” Acta Biomater. 7(1):31-56 (Jan. 2011). Epub Jul. 24, 2010. [cited by applicant]
Gaulding, et al., “Reversible Inter- and Intra-microgel Cross-linking Using Disulfides,” Macromolecules, 2012, vol. 45(1), pp. 39-45. [cited by applicant]
Hasegawa, Urara et al. “Nanogel-quantum dot hybrid nanoparticles for live cell imaging.” Biochemical and biophysical research communications 331(4):917-921 (2005). [cited by applicant]
Hegelson et al., “Hydrogel microparticles from lithographic processes: novel materials for fundamental and applied colloid science,” Curr. Opin. Colloid. Interface Sci. 16(2):106-117 (Apr. 1, 2011). [cited by applicant]
Heller et al., “inylcarbonates and vinylcarbamates: Biocompatible monomers for radical photopolymerization,” Journal of Polymer Science Part A: Polymer Chemistry 49, pp. 650-661 (Dec. 2, 2010). [cited by applicant]
Hermenegildo et al., “Hydrogel-based Magnetoelectric Microenvironments for Tissue Stimulation,” Colloidsand Surfaces B: Biointerfaces 181 1041-1047 (2019). [cited by applicant]
Higuchi, A., et al., “Design of polymeric materials for culturing human pluripotent stem cells: Progress toward feeder-free and xeno-free culturing,” Progress in Polymer Science, Jul. 2014, vol. 39 (7), pp. 1348-1374. [cited by applicant]
Hu and Messersmith, “Rational design of transglutaminase substrate peptides for rapid enzymatic formation of hydrogels,” . J Am. Chem. Soc. 125, 14298-14299 (Oct. 31, 2003). [cited by applicant]
Jain et al. Zwitterionic Hydrogels Based on a Degradable Disulfide Carboxybetaine Cross-Linker, Langmuir 2019, 35, 1864-1871 (Year: 2019). [cited by applicant]
Jin et al., “Overview of cell death signaling pathways” , Cancer Biology & Therapy, vol. 4, No. 2, Feb. 2, 2005, pp. 147-171, DOI: 10.4161/cbt.4.2.1508. [cited by applicant]
Kim, Jin-Woong et al., “Fabrication of Monodisperse Gel Shells and Functional Microgels in Microfluidic Devices,” Angew. Chem. Int. Ed. 46:819-1822 (2007). [cited by applicant]
Lee, Ki-Chang and Lee, Sang-Yun, “Preparation of Highly Cross-Linked, Monodisperse Poly (methyl methacrylate) Microspheres by Dispersion Polymerization; Part II. Semi-continuation Processes,” Macromolecular Research 6(4… [cited by applicant]
Liu, A.L., et al., “Methods for Generating Hydrogel Particles for Protein Delivery,” Annals of Biomedical Engineering, Jun. 2016, vol. 44 (6), pp. 1946-1958. [cited by applicant]
Liu, Z. et al., Recent Advances on Magnetic Sensitive Hydrogels in Tissue Engineering, Frontiers in Chemistry, vol. 8 , Article 124, pp. 1-17, (Mar. 2020). [cited by applicant]
Luchini, Alessandra et al. “Smart hydrogel particles: biomarker harvesting: one-step affinity purification, size exclusion, and protection against degradation.” Nano letters 8(1): 350-361 (2008). [cited by applicant]
Lutolf et al., “Synthetic matrix metalloproteinase-sensitive hydrogels for the conduction of tissue regeneration: engineering cell-invasion characteristics,” Proc Natl Acad Sci U S A 100(9):5413-8. (Apr. 29, 2003). Epub… [cited by applicant]
Martin, et al., “Elucidating the Structure of Membrane Proteins,” Bio Techniques, 2019, vol. 66 (4), pp. 167-170. [cited by applicant]
Martino et al., “Controlling integrin specificity and stem cell differentiation in 2D and 3D environments through regulation of fibronectin domain stability,” Biomaterials 30(6):1089-97 (Feb. 2009). Epub Nov. 22, 2008. [cited by applicant]
Martino et al., “Engineering the growth factor microenvironment with fibronectin domains to Promote Wound and Bone Tissue Healing,” Sci. Trans. Med. 3(100);100ra89, 10 pages (Sep. 14, 2011). [cited by applicant]
Mcdonald et al., “Fabrication of microfluidic systems in poly(dimethylsiloxane),” Electrophoresis 21 :27-40 (Jan. 1, 2000). First published: Dec. 29, 1999. [cited by applicant]
Pastor et al., “CD28 aptamers as powerful immune response modulators,” Mol Ther Nucleic Acids 2:e98, 9 pages (Jun. 11, 2013). [cited by applicant]
Patanarut, Alexis et al., “Synthesis and characterization of hydrogel particles containing Cibacron Blue F3G-A.” Colloids and Surfaces A: Physicochemical and Engineering Aspects 362(1):8-19 (2010). [cited by applicant]
Perez-Luna, V.H., et al., “Encapsulation of Biological Agents in Hydrogels for Therapeutic Applications,” Gels, vol. 4(61), pp. 1-30, (Jul. 11, 2018). [cited by applicant]
Petka et al., “Reversible hydrogels from self-assembling artificial proteins,” Science 281(5375):389-392 (Jul. 1998). [cited by applicant]
Poirier et al., “CD28-specific immunomodulating antibodies: what can be learned from experimental models?” American Journal of Transplantation 12(7): 1682-1690 (Jul. 2012). Epub Apr. 4, 2012. [cited by applicant]
Porto, “Polymer Biocompatibility,” Polymerization, Dr. Ailton De Souza Gomes (Ed.), 17 pages (2012). [cited by applicant]
Proll, Guenther et al. “Potential of label-free detection in high-content-screening applications.” Journal of Chromatography A 1116(1):2-8 (2007). [cited by applicant]
Qui et al., “Apoptosis of multiple myeloma cells induced by agonist monoclonal antibody against human CD28,” Cell Immunol. 236(1-2): 154-60 (Jul.-Aug. 2005). Epub Sep. 26, 2005, 2005. [cited by applicant]
Riley et al., Human T regulatory cell therapy: take a billion or so and call me in the morning, Immunity 30(5):656-65. (May 2009). [cited by applicant]
Shastri, V.P., et al., “Non-Degradable Biocompatible Polymers in Medicine: Past, Present and Future”, Current Pharmaceutical Biotechnology, Bentham Science Publishers, Nl, vol. 4, No. 5, Jan. 1, 2003, pp. 331-337. [cited by applicant]
Simpson, et al., “Protein Folding and Assembly in Confined Environments: Implications for Protein Aggregation in Hydrogels and Tissues,” Biotechnology Advances, 2020, vol. 42, pp. 1-11. [cited by applicant]
Sugiura et al., “Effect of Channel Structure on Microchannel Emulsification,” Languimir 18(15): 5708-5712 (Jun. 22, 2002). [cited by applicant]
Tomczak, Nikodem et al., “Designer polymer-quantum dot architectures.” Progress in Polymer Science 34:393-430 (2009). [cited by applicant]
Ugelstad, J. and Mork, P.C., “Swelling of Oligomer-Polymer Particles. New Methods of Preparation of Emulsions and Polymer Dispersions,” Advances in Colloid and Interface Sciences, 13:101-140 (1980). [cited by applicant]
Wallberg et al., “Analysis of Apoptosis and Necroptosis by Fluorescence-Activated Cell Sorting,” Cold Spring Harbor Protocol, vol. 2016, No. 4, Apr. 1, 2016, 7 pages. [cited by applicant]
Weinkove et al., “Selecting costimulatory domains for chimeric antigen receptors: functional and clinical considerations,” Clin Transl Immunology 8(5):el049, p. 1-14 (May 11, 2019). [cited by applicant]
Xu et al., “Hyaluronic Acid-Based Hydrogels: From a Natural Polysaccharide to Complex Networks,” Soft Matter. 8(12):3280-3294 (Mar. 2012). [cited by applicant]
Yadav and Redmond, “Current Clinical Trial Landscape of OX40 Agonists,” Curr Oncol Rep. 24(7):951-960 (Jul. 2022). [cited by applicant]
Yang et al., “IDBD: Infectious Disease Biomarker Database,” Nucleic Acid Res. 36:D455-D460 (Jan. 2008). Published online Nov. 3, 2007. [cited by applicant]
Zhang et al., “Protein engineering with unnatural amino acids,” Current Opinion in Structural Biology 23(4):581-587 (Aug. 2013). [cited by applicant]