Systems, methods, and compositions for prevention of tissue adhesion
A blended electrophilic material with a first component having a functionality of at least three and a second component having a functionality of two is mixed with a nucleophilic material. The blended electrophilic material cross-links with the nucleophilic material to form a non-liquid, three dimensional structure which can applied, e.g., as an adhesion barrier.
1. A method comprising:
providing a blended electrophilic material comprising a first poly(ethylene glycol) PEG component having a functionality of at least three and a second PEG component having a functionality of two;
providing a nucleophilic material, separate from the blended electrophilic material, which upon mixing with the blended electrophilic material, cross-links with the blended electrophilic material to form a non-liquid, three dimensional structure; and
mixing the blended electrophilic material and the nucleophilic material to form the non-liquid, three dimensional structure.
2. A method according to claim 1 , wherein the non-liquid, three dimensional structure forms upon tissue.
3. A method according to claim 2 , wherein the non-liquid, three dimensional structure comprises an adhesion barrier.
4. A method according to claim 1 , wherein the blended electrophilic material comprises a first component having a PEG compound having a 4-arm functionality and a second component having a PEG compound having a linear functionality.
5. A method according to claim 4 , wherein the PEG compound having 4-arm functionality comprises about 60% w/w of the blended electrophilic material.
6. A method according to claim 4 , wherein the PEG compound having linear functionality comprises about 40% w/w of the blended electrophilic material.
7. A method according to claim 1 , wherein said nucleophilic material comprises a protein, which is from a natural source.
8. A method according to claim 1 , wherein the first PEG component comprises between 10% to 90% w/w of the blended electrophilic material.
9. A method according to claim 1 , wherein the first PEG component has a functionality of four.
10. A method according to claim 1 , wherein the first PEG component comprises about 50% w/w of the blended electrophilic material.
11. A method according to claim 1 , further including a buffer material.
12. A method according to claim 7 , wherein the protein comprises natural serum albumin.
13. A method according to claim 12 , wherein the natural serum albumin is at a concentration of 25% or less.
14. A method according to claim 12 , wherein the natural serum albumin is at a concentration of about 15%.