Fusing method of substrate layer, manufacturing method of microfluidic chip and fusing apparatus of substrate layer

1. A method of fusing a first substrate layer and a second substrate layer comprising:

treating a joining surface of one of the first substrate layer and the second substrate layer formed from a resin using an organic solvent having solubility with respect to the resin;

heating the first substrate layer and the second substrate layer after the organic solvent treatment at a heating temperature less than a glass transition temperature or a softening point temperature of the resin and crimping the heated substrate layers together;

removing the organic solvent remaining on the crimped substrate layers; and

pressurizing the crimped substrate layers after removing the organic solvent while heating at a temperature which is higher than the heating temperature and lower than the glass transition temperature or the softening point temperature of the resin.

2. The method of fusing a first substrate layer and a second substrate layer according to claim 1 ,

wherein a region which becomes a reaction field or an analysis field of a substance is formed on the substrate layer treated with the organic solvent.

3. The method of fusing a first substrate layer and a second substrate layer according to claim 2 ,

wherein the substrate layer treated with the organic solvent has a minute level difference at the joining surface.

4. The method of fusing a first substrate layer and a second substrate layer according to claim 3 ,

wherein the level difference is present between the joining surface and an electrode formed at the joining surface.

5. The method of fusing a first substrate layer and a second substrate layer according to claim 4 ,

wherein, in the treating, the joining surface is treated using a vaporized form of the organic solvent.

6. The method of fusing a first substrate layer and a second substrate layer according to claim 5 ,

wherein in the heating and crimping and/or the pressurizing, via a buffer material, an opposite side surface to the joining surface of the substrate layer treated with the organic solvent is pressed.

7. A manufacturing method of a microfluidic chip comprising:

treating a joining surface of one of a first substrate layer and a second substrate layer formed from a resin, having a region which becomes a reaction field or an analysis field of a substance, using an organic solvent having solubility with respect to the resin;

heating the first substrate layer and the second substrate layer after the organic solvent treatment at a heating temperature less than a glass transition temperature or a softening point temperature of the resin and crimping the heated substrate layers together;

removing the organic solvent remaining on the crimped substrate layers; and

pressurizing the crimped substrate layers after removing the organic solvent while heating at a temperature which is higher than the heating temperature and lower than the glass transition temperature or the softening point temperature of the resin.

8. A manufacturing method of a microfluidic chip comprising:

treating a joining surface of one of a first substrate layer and a second substrate layer formed from a resin, having a minute level difference at the joining surface, using an organic solvent having solubility with respect to the resin;

heating the first substrate layer and the second substrate layer after the organic solvent treatment at a heating temperature less than a glass transition temperature or a softening point temperature of the resin and crimping the heated substrate layers together;

removing the organic solvent remaining on the crimped substrate layers; and

pressurizing the crimped substrate layers after removing the organic solvent while heating at a temperature which is higher than the heating temperature and lower than the glass transition temperature or the softening point temperature of the resin,

wherein the level difference is sealed by the resin.

9. A fusing apparatus of a substrate layer comprising:

a dissolving unit that treats a joining surface of one of a first substrate layer and a second substrate layer formed from a resin using an organic solvent having solubility with respect to the resin;

a crimping unit that heats the first substrate layer and the second substrate layer after the organic solvent treatment at a heating temperature less than a glass transition temperature or a softening point temperature of the resin and crimps the heated substrate layers together;

a drying mechanism that removes the organic solvent remaining on the crimped substrate layers; and

a pressurizing unit that pressurizes the crimped substrate layers after removing the organic solvent while heating at a temperature which is higher than the heating temperature and lower than the glass transition temperature of the resin in the crimping unit.