Polypeptides having endoglucanase activity and polynucleotides encoding same
The present invention relates to isolated polypeptides having endoglucanase activity and isolated polynucleotides encoding the polypeptides. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the polynucleotides as well as methods of producing and using the polypeptides.
1. A method for degrading a cellulosic material, comprising: treating the cellulosic material with an enzyme composition comprising a polypeptide having endoglucanase activity, wherein the polypeptide having endoglucanase activity is selected from the group consisting of:
(a) a polypeptide having endoglucanase activity with at least 90% sequence identity to amino acids 26 to 463 of SEQ ID NO: 4;
(b) a polypeptide having endoglucanase activity encoded by a polynucleotide with at least 90% sequence identity to nucleotides 76 to 1389 of SEQ ID NO: 3 or the cDNA sequence thereof;
(c) a polypeptide comprising a catalytic domain having endoglucanase activity with at least 90% sequence identity to amino acids 26 to 400 of SEQ ID NO: 4; and
(d) a polypeptide comprising a catalytic domain having endoglucanase activity encoded by a polynucleotide with at least 90% sequence identity to nucleotides 76 to 1200 of SEQ ID NO: 3.
2. The method of claim 1 , wherein the polypeptide having endoglucanase activity comprises amino acids 26 to 463 of SEQ ID NO: 4.
3. The method of claim 1 , wherein the catalytic domain having endoglucanase activity comprises amino acids 26 to 400 of SEQ ID NO: 4.
4. The method of claim 1 , wherein the enzyme composition further comprises one or more enzymes selected from the group consisting of a cellulase, a GH61 polypeptide having cellulolytic enhancing activity, a hemicellulase, an esterase, an expansin, a laccase, a ligninolytic enzyme, a pectinase, a peroxidase, a protease, and a swollenin.
5. The method of claim 1 , further comprising recovering the degraded cellulosic material.
6. The method of claim 5 , wherein the degraded cellulosic material is a sugar.
7. The method of claim 6 , wherein the sugar is selected from the group consisting of glucose, xylose, mannose, galactose, and arabinose.
8. A method for producing a fermentation product, comprising:
(a) saccharifying a cellulosic material with an enzyme composition comprising a polypeptide having endoglucanase activity;
(b) fermenting the saccharified cellulosic material with one or more fermenting microorganisms to produce the fermentation product; and
(c) recovering the fermentation product from the fermentation,
wherein the polypeptide having endoglucanase activity is selected from the group consisting of:
(i) a polypeptide having endoglucanase activity with at least 90% sequence identity to amino acids 26 to 463 of SEQ ID NO: 4;
(ii) a polypeptide having endoglucanase activity encoded by a polynucleotide with at least 90% sequence identity to nucleotides 76 to 1389 of SEQ ID NO: 3 or the cDNA sequence thereof;
(iii) a polypeptide comprising a catalytic domain having endoglucanase activity with at least 90% sequence identity to amino acids 26 to 400 of SEQ ID NO: 4; and
(iv) a polypeptide comprising a catalytic domain having endoglucanase activity encoded by a polynucleotide with at least 90% sequence identity to nucleotides 76 to 1200 of SEQ ID NO: 3.
9. The method of claim 8 , wherein the polypeptide having endoglucanase activity comprises amino acids 26 to 463 of SEQ ID NO: 4.
10. The method of claim 8 , wherein the catalytic domain having endoglucanase activity comprises amino acids 26 to 400 of SEQ ID NO: 4.
11. The method of claim 8 , wherein the enzyme composition further comprises one or more enzymes selected from the group consisting of a cellulase, a GH61 polypeptide having cellulolytic enhancing activity, a hemicellulase, an esterase, an expansin, a laccase, a ligninolytic enzyme, a pectinase, a peroxidase, a protease, and a swollenin.
12. The method of claim 8 , wherein steps (a) and (b) are performed simultaneously in a simultaneous saccharification and fermentation.
13. The method of claim 8 , wherein the fermentation product is an alcohol, an alkane, a cycloalkane, an alkene, an amino acid, a gas, isoprene, a ketone, an organic acid, or polyketide.
14. A method of fermenting a cellulosic material, comprising: fermenting the cellulosic material with one or more fermenting microorganisms, wherein the cellulosic material is saccharified with an enzyme composition comprising a polypeptide having endoglucanase activity, wherein the polypeptide having endoglucanase activity is selected from the group consisting of:
(a) a polypeptide having endoglucanase activity with at least 90% sequence identity to amino acids 26 to 463 of SEQ ID NO: 4;
(b) a polypeptide having endoglucanase activity encoded by a polynucleotide with at least 90% sequence identity to nucleotides 76 to 1389 of SEQ ID NO: 3 or the cDNA sequence thereof;
(c) a polypeptide comprising a catalytic domain having endoglucanase activity with at least 90% sequence identity to amino acids 26 to 400 of SEQ ID NO: 4; and
(d) a polypeptide comprising a catalytic domain having endoglucanase activity encoded by a polynucleotide with at least 90% sequence identity to nucleotides 76 to 1200 of SEQ ID NO: 3.
15. The method of claim 14 , wherein the polypeptide having endoglucanase activity comprises amino acids 26 to 463 of SEQ ID NO: 4.
16. The method of claim 14 , wherein the catalytic domain having endoglucanase activity comprises amino acids 26 to 400 of SEQ ID NO: 4.
17. The method of claim 14 , wherein the enzyme composition further comprises one or more enzymes selected from the group consisting of a cellulase, a GH61 polypeptide having cellulolytic enhancing activity, a hemicellulase, an esterase, an expansin, a laccase, a ligninolytic enzyme, a pectinase, a peroxidase, a protease, and a swollenin.
18. The method of claim 14 , wherein the fermenting of the cellulosic material produces a fermentation product.
19. The method of claim 18 , further comprising recovering the fermentation product from the fermentation.
20. The method of claim 19 , wherein the fermentation product is an alcohol, an alkane, a cycloalkane, an alkene, an amino acid, a gas, isoprene, a ketone, an organic acid, or polyketide.
21. The method of claim 1 , wherein the polypeptide having endoglucanase activity has at least 95% sequence identity to amino acids 26 to 463 of SEQ ID NO: 4.
22. The method of claim 1 , wherein the polypeptide having endoglucanase activity has at least 97% sequence identity to amino acids 26 to 463 of SEQ ID NO: 4.
23. The method of claim 1 , wherein the polypeptide having endoglucanase activity has at least 99% sequence identity to amino acids 26 to 463 of SEQ ID NO: 4.
24. The method of claim 1 , wherein the catalytic domain having endoglucanase activity has at least 95% sequence identity to amino acids 26 to 400 of SEQ ID NO: 4.
25. The method of claim 1 , wherein the catalytic domain having endoglucanase activity has at least 97% sequence identity to amino acids 26 to 400 of SEQ ID NO: 4.
26. The method of claim 1 , wherein the catalytic domain having endoglucanase activity has at least 99% sequence identity to amino acids 26 to 400 of SEQ ID NO: 4.
27. The method of claim 8 , wherein the polypeptide having endoglucanase activity has at least 95% sequence identity to amino acids 26 to 463 of SEQ ID NO: 4.
28. The method of claim 8 , wherein the polypeptide having endoglucanase activity has at least 97% sequence identity to amino acids 26 to 463 of SEQ ID NO: 4.
29. The method of claim 8 , wherein the polypeptide having endoglucanase activity has at least 99% sequence identity to amino acids 26 to 463 of SEQ ID NO: 4.
30. The method of claim 8 , wherein the catalytic domain having endoglucanase activity has at least 95% sequence identity to amino acids 26 to 400 of SEQ ID NO: 4.
31. The method of claim 8 , wherein the catalytic domain having endoglucanase activity has at least 97% sequence identity to amino acids 26 to 400 of SEQ ID NO: 4.
32. The method of claim 8 , wherein the catalytic domain having endoglucanase activity has at least 99% sequence identity to amino acids 26 to 400 of SEQ ID NO: 4.
33. The method of claim 14 , wherein the polypeptide having endoglucanase activity has at least 95% sequence identity to amino acids 26 to 463 of SEQ ID NO: 4.
34. The method of claim 14 , wherein the polypeptide having endoglucanase activity has at least 97% sequence identity to amino acids 26 to 463 of SEQ ID NO: 4.
35. The method of claim 14 , wherein the polypeptide having endoglucanase activity has at least 99% sequence identity to amino acids 26 to 463 of SEQ ID NO: 4.
36. The method of claim 14 , wherein the catalytic domain having endoglucanase activity has at least 95% sequence identity to amino acids 26 to 400 of SEQ ID NO: 4.
37. The method of claim 14 , wherein the catalytic domain having endoglucanase activity has at least 97% sequence identity to amino acids 26 to 400 of SEQ ID NO: 4.
38. The method of claim 14 , wherein the catalytic domain having endoglucanase activity has at least 99% sequence identity to amino acids 26 to 400 of SEQ ID NO: 4.