Analyte sensing biointerface
Disclosed herein is an analyte sensing biointerface that comprises a sensing electrode incorporated within a non-conductive matrix comprising a plurality of passageways extending through the matrix to the sensing electrode. Also disclosed herein are methods of manufacturing a sensing biointerface and methods of detecting an analyte within tissue of a host using an analyte sensing biointerface.
1. A transcutaneous continuous glucose sensor system, comprising:
a substantially planar sensor, the sensor comprising:
a first non-conductive layer;
a first conductive layer associated with a first electrode, wherein the first conductive layer is disposed at least in part over the first non-conductive layer;
a second non-conductive layer disposed at least in part over the first conductive layer;
a second conductive layer associated with a second electrode, wherein the second conductive layer is disposed at least in part over the second non-conductive layer;
a third non-conductive layer disposed at least in part over the second conductive layer;
a third conductive layer associated with a third electrode, wherein the third conductive layer is disposed at least in part over the third non-conductive layer; and
a membrane disposed over at least a portion of a working electrode;
wherein at least one of the first electrode, the second electrode, or the third electrode is the working electrode, and wherein the working electrode is configured to measure a signal indicative of a glucose concentration.
2. The transcutaneous continuous glucose sensor system of claim 1 , wherein the membrane comprises an enzyme.
3. The transcutaneous continuous glucose sensor system of claim 2 , wherein the membrane further comprises a resistance domain disposed over the enzyme.
4. The transcutaneous continuous glucose sensor system of claim 1 , wherein the membrane comprises a bioactive agent.
5. The transcutaneous continuous glucose sensor system of claim 1 , wherein the membrane is substantially planar.
6. The transcutaneous continuous glucose sensor system of claim 1 , wherein at least a portion of the first non-conductive layer is in physical contact with at least a portion of the first conductive layer.
7. The transcutaneous continuous glucose sensor system of claim 1 , further comprising sensor electronics configured to electrically couple to the working electrode.
8. The transcutaneous continuous glucose sensor system of claim 1 , wherein at least a portion of the first non-conductive layer is physically separate from the first conductive layer.
9. The transcutaneous continuous glucose sensor system of claim 1 , further comprising sensor electronics having one or more contacts for operably connecting to the working electrode.