IP Library › Granted Patent US 12,203,087
Granted Patent B2
US 12,203,087 · App. 18/409,596 · Granted Jan 21, 2025

Enzymes and methods for producing omega-3 fatty acids

Inventors: James Robertson Petrie (Bywong, AU); Anne Maree Mackenzie (Hawker, AU); Qing Liu (Giralang, AU); Pushkar Shrestha (Lawson, AU); Peter David Nichols (West Hobart, AU); Susan Irene Ellis Blackburn (Battery Point, AU); Maged Peter Mansour (Gladstone Park, AU); Stanley Suresh Robert (Oyster Cove, AU); Dion Matthew Frederick Frampton (Oyster Cove, AU); Xue-Rong Zhou (Bonnet Hill Harrison, AU); Surinder Pal Singh (Downer, AU); Craig Christopher Wood (Dickson, AU)
Assignees: COMMONWEALTH SCIENTIFIC AND INDUSTRIAL RESEARCH ORGANISATION; GRAINS RESEARCH AND DEVELOPMENT CORPORATION
C12N15/8247C12P7/6427C12P7/6432C12P7/6434C12P7/6472C12Y602/01003
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Quick Facts
Patent No.
US 12,203,087
App. No.
18/409,596
Granted
Jan 21, 2025
Kind
B2
Abstract

The present invention relates to methods of synthesizing long-chain polyunsaturated fatty acids, especially eicosapentaenoic acid, docosapentaenoic acid and docosahexaenoic acid, in recombinant cells such as yeast or plant cells. Also provided are recombinant cells or plants which produce long-chain polyunsaturated fatty acids. Furthermore, the present invention relates to a group of new enzymes which possess desaturase or elongase activity that can be used in methods of synthesizing long-chain polyunsaturated fatty acids. In particular, the present invention provides ω3 destaurases, Δ5 elongases and Δ6 desaturases with novel activities. Also provided are methods and DNA constructs for transiently and/or stably transforming cells, particularly plant cells, with multiple genes.

Claims (15)

1. A process for producing oil comprising eicosapentaenoic acid (EPA) and docosapentaenoic acid (DPA), the process comprising the steps of (i) obtaining seed of Brassicaceae plant, the seed comprising a total fatty acid content and exogenous polynucleotides encoding a microalgal Δ6-desaturase which has greater Δ6-desaturase activity on α-linolenic acid (ALA) joined to CoA (ALA-CoA) as fatty acid substrate than on ALA joined to the sn-2 position of phosphatidylcholine (PC) as the fatty acid substrate, a Δ6-elongase, and a microalgal Δ5-desaturase, wherein each polynucleotide is operably linked to one or more promoters that direct expression of said polynucleotides during the development of the seed, wherein ALA, stearidonic acid (SDA), eicosatetraenoic acid (ETA), EPA and DPA are each present at a level in the total fatty acid content of the seed, each level being expressed as a percentage of the total fatty acid content, such that the sum of the percentages for EPA and DPA divided by the sum of the percentages for ALA, SDA, ETA, EPA and DPA, expressed as a percentage, is at least 17.3%, and

(ii) extracting oil from the seed, thereby producing the oil comprising EPA and DPA.

2. The process of claim 1 , wherein arachidonic acid (ARA), EPA, DPA and docosahexaenoic acid (DHA), if present in the total fatty acid content, together comprise 15% of the total fatty acid content in the seed.

3. The process of claim 1 , wherein the Δ6-desaturase is an Ostreococcus tauri Δ6-desaturase, Mantoniella squamata Δ 6-desaturase or Micromonas pusilla Δ 6-desaturase.

4. The process of claim 1 , wherein the Δ6-elongase is a Physcomitrella patens Δ 6-elongase or a Pyramimonas CS-0140 Δ6-elongase.

5. The process of claim 1 , wherein the Δ5-desaturase is a Thraustochytrium sp Δ5-desaturase.

6. The process of claim 1 , wherein the Δ6-desaturase is an Ostreococcus tauri, Mantoniella squamata or Micromonas pusilla Δ 6-desaturase, the Δ6-elongase is a Physcomitrella patens Δ6-elongase, and the Δ5-desaturase is a Thraustochytrium sp Δ5-desaturase.

7. The process of claim 1 , wherein the seed further comprises an exogenous polynucleotide encoding a Δ15-desaturase.

8. The process of claim 7 , wherein the Δ15-desaturase is a Perilla frutescens Δ15-desaturase.

9. The process of claim 1 , wherein the Brassicaceae plant is Camelina sativa.

10. The process of claim 1 , which comprises a step of purifying the extracted oil.

11. The process of claim 1 , which further comprises a step of hydrolysis of the extracted oil with a strong base to release free fatty acids.

12. The process of claim 1 , which further comprises a step of fractionation of the extracted oil.

13. The process of claim 1 , which further comprises a step of distillation of the extracted oil.

14. The process of claim 1 , which further comprises a step of degumming, caustic refining, bleaching, and/or deodorization of the extracted oil.

Continuity (6)
Continuation 16860936 · Apr 28, 2020
Continuation 15642223 · Jul 5, 2017
Continuation 14332202 · Jul 15, 2014
Continuation 13129940
Provisional Application 61270710 · Jul 9, 2009
Related Publication 20240150780A1 · May 9, 2024
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Sayanova, O.V. and Napier, J.A., (2004) “Eicosapentaenoic Acid: Biosynthetic Routes and the Potential for Synthesis in Transgenic Plants,” Phytochemistry, 65(2): 147-158. [cited by applicant]
Abbadi, A., et al., (2001) “Transgenic Oilseeds as Sustainable Source of Nutritionally Relevant C20 and C22 Polyunsaturated Fatty Acids?” European Journal of Lipid Science and Technology, 103(2): 106-113. [cited by applicant]
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Sequence alignment demonstrating that the amino acid sequence of GenBank accession number AY055117 was found to have the same length as protein SEQ ID No.: 64 of WO 2005/103253, filed as “D18” in connection with Opposit… [cited by applicant]
Sequence alignment demonstrating that the amino acid sequence of GenBank accession number AY055118 was found to have the same length as protein SEQ ID No.: 64 of WO 2005/103253, filed as “D19” in connection with Opposit… [cited by applicant]
Reference formula used for the calculation of the melting temperature as regards sequences hybridizing under high stringency conditions, filed as “D20” in connection with Opposition to European Patent No. EP1756280 (App… [cited by applicant]
Abbadi, A., et al., (2004) “Biosynthesis of Very-Long-Chain Polyunsaturated Fatty Acids in Transgenic Oilseeds: Constraints on Their Accumulation,” The Plant Cell, 16(10): 2734-2748. [cited by applicant]
Mar. 29, 2016 Response to the Notice of Opposition, filed by CSIRO in connection with Opposition to European Patent No. EP1756280 (Application No. EP05733657.0). [cited by applicant]
Jul. 18, 2016 EPO Communication providing Summons to attend Oral Proceedings and Preliminary Opinion, issued in connection with Opposition to European Patent No. EP1756280 (Application No. EP05733657.0). [cited by applicant]
Jan. 9, 2017 Written Submissions under Rule 116 EPC filed in response to the Summons to Oral Proceedings, filed by CSIRO in connection with Opposition to European Patent No. EP1756280 (Application No. EP05733657.0). [cited by applicant]
Auxiliary Request I claims, filed with Jan. 9, 2017 Written Submissions under Rule 116 EPC, in response to the Summons to Oral Proceedings, in connection with Opposition to European Patent No. EP1756280 (Application No.… [cited by applicant]
Jan. 9, 2017 Written Submissions under Rule 116 EPC filed in response to the Summons to Oral Proceedings, filed by BASF in connection with European Patent Application No. EP05733657.0. [cited by applicant]
Huang Y. S., Pereira S. L., Leonard A. E. (2004) “Enzymes for transgenic biosynthesis of long-chain polyunsaturated fatty acids” Biochimie, vol. 86, No. 11, p. 793-798. [cited by applicant]
Graham, et al. (2004) “The use of very long chain polyunsaturated fatty acids to ameliorate metabolic syndrome: transgenic plants as an alternative sustainable source to fish oils” Nutrition Bulletin, vol. 29, p. 228-23… [cited by applicant]
Napier JA, Beaudoin F, Michaelson LV, Sayanova O. (2004) “The production of long chain polyunsaturated fatty acids in transgenic plants by reverse-engineering.” Biochimie. Nov. 2004 86(11):785-92. [cited by applicant]
Napier JA, Beaudoin F, Sayanova O. (2005) “Reverse engineering of long-chain polyunsaturated fatty acid biosynthesis into transgenic plants” European Journal of Lipid Science and Technology 107(4):249-255. [cited by applicant]
Cahoon, E. B., Marillia, E., Stecca, K. L., Hall, S. E., Taylor, D. C., & Kinney, A. J. (2000). Production of fatty acid components of meadowfoam oil in somatic soybean embryos. [cited by applicant]
Kinney AJ, Cahoon EB, Hitz WD. “Manipulating desaturase activities in transgenic crop plants.” Biochem Soc Trans. Nov. 30, 2002(Pt 6):1099-103. [cited by applicant]
Voelker T1, Kinney Aj. “Variations in the Biosynthesis of Seed-Storage Lipids.” Annu Rev Plant Physiol Plant Mol Biol. Jun. 2001 52:335-361. [cited by applicant]
Surinder Singh (2010) “Engineering Complex Fatty Acid Pathways in Seeds: The EPA/DHA Example”, submitted as “D31” subsequently renumbered as “D34” in connection with Opposition to European Patent No. EP1756280 (Applicat… [cited by applicant]
Materials and methods, results and conclusions for experimental data provided by the Opponent. The Opponent's experiments repeated and expanded on the experiment described in Example 5 of the opposed patent EP 1756280 (… [cited by applicant]
Feb. 21, 2017 Additional Submissions, including list of all documents cited to date and documents “D36”, “D37” and “D38” filed in connection with Opposition to European Patent No. EP1756280 (Application No. EP05733657.0… [cited by applicant]
Mar. 9, 2017 Information about the result of Oral Proceedings, filed in connection with Opposition to European Patent No. EP1756280 (Application No. EP05733657.0). [cited by applicant]
List of documents cited by CSIRO and BASF as of Jan. 16, 2017, in connection Opposition to European Patent No. EP1756280 (Application No. EP05733657.0). [cited by applicant]
Aug. 23, 2017 Appeal Brief, filed by BASF in connection with Opposition to European Patent No. EP1756280. [cited by applicant]
Aug. 30, 2017 Correction to Aug. 23, 2017 Appeal Brief, filed by BASF in connection with Opposition to European Patent No. EP1756280. [cited by applicant]
Feature-structured claim 1, submitted as “D39” by BASF with Aug. 23, 2017 Appeal Brief, filed by BASF in connection with Opposition to European Patent No. EP1756280. [cited by applicant]
Heinz (2002). “Docosahexaenoic acid (DHA) in transgenic oilseeds: which approach will be successful first?” European Journal of Lipid Science and Technology 2002, 104:1-2. [cited by applicant]
Das et al. (2000) “Polyunsaturated fatty acid-specific elongation enzymes”, Biochemical Society Transactions 28(6):658-660. [cited by applicant]
Domergue, F., et al., (2002) “Cloning and Functional Characterization of Phaeodactylum Tricornutum Front-End Desaturases Involved in Eicosapentaenoic Acid Biosynthesis,” European Journal of Biochemistry, 269(16):4105-41… [cited by applicant]
Presentation “Engineering of LCPUFA biosynthesis” presented by Prof. Heinz at the 1st European Symposium on Plant Lipids held Sep. 10-13, 2003 in Aachen, submitted as “D44” by BASF with Aug. 23, 2017 Appeal Brief, filed… [cited by applicant]
Qi, et al., (2004) “Production of Very Long Chain Polyunsaturated Omega-3 and Omega-6 Fatty Acids in Plants”, Nature Biotechnology 22:739-745 (published online May 16, 2004). [cited by applicant]
Evidence of the publication date of D44, submitted as “D44a” by BASF with Aug. 23, 2017 Appeal Brief, filed by BASF in connection with Opposition to European Patent No. EP1756280. [cited by applicant]
Hoffman et al., (2008) “Metabolic Engineering of ω3-Very Long Chain Polyunsaturated Fatty Acid Production by an Exclusively Acyl-CoA-dependent Pathway”, The Journal of Biological Chemistry, 283:22352-22362. [cited by applicant]
Expert declaration from Dr. Domergue, submitted as “D47” by BASF with Aug. 23, 2017 Appeal Brief, filed by BASF in connection with Opposition to European Patent No. EP1756280. [cited by applicant]
Leonard et al. (2004) “Elongation of long-chain fatty acids”, Progress in Lipid Research 43:36-54. [cited by applicant]
Experimental data relating to EPA production in different plants, including experimental details, results, a discussion and conclusion, submitted as “D49” by BASF with Aug. 23, 2017 Appeal Brief, filed by BASF in connec… [cited by applicant]
Media used in [cited by applicant]
Experimental data on EPA production of segregating seeds, submitted as “D50” by BASF with Aug. 23, 2017 Appeal Brief, filed by BASF in connection with Opposition to European Patent No. EP1756280. [cited by applicant]
Experimental data on EPA production of homozygous seeds, submitted as “D51” by BASF with Aug. 23, 2017 Appeal Brief, filed by BASF in connection with Opposition to European Patent No. EP1756280. [cited by applicant]
Dec. 28, 2018 Examination Report, issued in connection with Canadian Patent Application No. 2,743,880. [cited by applicant]
Jan. 4, 2018 Response of Commonwealth Scientific and Industrial Research Organisation (Patentee) to Statement of Appeal by BASF SE (Opponent) in connection with Opposition to European Patent No. EP1756280, including cop… [cited by applicant]
Jan. 4, 2018 Response of BASF SE to Grounds of Appeal filed by Commonwealth Scientific and Industrial Research Organisation on Aug. 22, 2017 in connection with Opposition to European Patent No. EP1756280. [cited by applicant]
Sayanova et al., The role of delta (6)-desaturase acyl-carrier specificity in the efficient synthesis of long-chain polyunsaturated fatty acids in transgenic plants. Plant Biotechnology Journal, (2012) 10:195-206. [cited by applicant]
Connor, W. E. (2000). Importance of n-3 fatty acids in health and disease. The American journal of clinical nutrition, 71(1), 171S-175S. [cited by applicant]
Apr. 20, 2018 Amended Complaint for Declaratory Judgment filed by BASF Plant Science, LP in connection with U.S. Pat. No. 7,807,849, issued Oct. 5, 2010 (Singh et al.); U.S. Pat. No. 8,106,226, issued Jan. 31, 2012 (Sin… [cited by applicant]
Response to Examination Report filed Apr. 23, 2018 in connection with counterpart Indian Patent Application No. 3829/CHENP/2011. [cited by applicant]
May 16, 2018 Office Action issued in connection with Argentinian Patent Application No. 20090104445 and its English translation. [cited by applicant]
May 8, 2018 Office Action issued in connection with Japanese Application No. 2015- 134711 and its English translation. [cited by applicant]
Jun. 27, 2018 Response to Examination Report dated Dec. 28, 2017, issued in connection with Canadian Patent Application No. 2,743,880. [cited by applicant]
Jun. 26, 2018 Response to Communication under Rule 69 and Invitation under Rule 70a(1) EPC dated Jan. 4, 2018, issued in connection with European Patent Application No. 17182502.9. [cited by applicant]
Oct. 9, 2018 First Examination Report issued in connection with Canadian Patent Application No. 3,015,426. [cited by applicant]
Nov. 12, 2018 Office Action issued in connection with Argentine Patent Application No. 20090104445 including English language translation thereof. [cited by applicant]
Nov. 21, 2018 First Examination Report issued in connection with European Patent Application No. 17182502.9. [cited by applicant]
Jan. 8, 2019 Response to the Oct. 9, 2018 First Examination Report filed in connection with Canadian Patent Application No. 3015426. [cited by applicant]
Dec. 19, 2018 Fourth Examination Report issued in connection with Canadian Patent Application No. 2,743,880. [cited by applicant]
Feb. 25, 2019 Second Examination Report issued in connection with Canadian Patent Application No. 3015426. [cited by applicant]
Mar. 25, 2019 Office Action issued in connection with Argentine Patent Application No. 20090104445 including English language translation thereof. [cited by applicant]
May 24, 2019 Response to the Feb. 25, 2019 Second Examination Report filed in connection with Canadian Patent Application No. 3015426. [cited by applicant]
May 31, 2019 Response to the Nov. 21, 2018 First Examination Report filed in connection with European Patent Application No. 17182502.9. [cited by applicant]
Jan. 3, 2019 First Amended Counterclaims filed by Commonwealth Scientific and Industrial Research Corporation, Grains Research and Development Corporation, and Nuseed Pty. Ltd. in the United States District Court for th… [cited by applicant]
Jan. 11, 2019 BASF Plant Science, LP's Answer and Amended Affirmative Defenses to Counterclaimants' Amended Counterclaims and BASF Plant Science, LP and BASF Plant Science GMBH's Amended Counterclaims Against Countercla… [cited by applicant]
Feb. 27, 2019 Opening Claim Construction Brief of Cargill, Incorporated, in the United States District Court for the Eastern District of Virginia Norfolk Division, Civil Action No. 2:17-cv-00503-HCM-LRL. [cited by applicant]
Feb. 27, 2019 BASF Plant Science, LP's And BASF Plant Science GMBH's Opening Claim Construction Brief, in the United States District Court for the Eastern District of Virginia Norfolk Division, Civil Action No. 2:17-cv-… [cited by applicant]
Feb. 27, 2019 Counterclaimants Commonwealth Scientific and Industrial Research Organisation, Grains Research and Development Corp., and Nuseed Pty Ltd.'s Initial Markman Brief, in the United States District Court for th… [cited by applicant]
Mar. 13, 2019 Responsive Claim Construction Brief of Cargill, Incorporated, in the United States District Court for the Eastern District of Virginia Norfolk Division, Civil Action No. 2:17-cv-00503-HCM-LRL. [cited by applicant]
Mar. 13, 2019 BASF Plant Science, LP's And BASF Plant Science GMBH's Responsive Claim Construction Brief, in the United States District Court for the Eastern District of Virginia Norfolk Division, Civil Action No. 2:17-… [cited by applicant]
Mar. 13, 2019 Counterclaimants Commonwealth Scientific and Industrial Research Organisation, Grains Research and Development Corp., and Nuseed Pty Ltd.'s Rebuttal Markman Brief, in the United States District Court for t… [cited by applicant]
Apr. 17, 2019 Counterclaimants Commonwealth Scientific and Industrial Research Organisation, Grains Research and Development Corp., and Nuseed Pty Ltd.'s Supplemental Brief on Term 9, in the United States District Court… [cited by applicant]
Apr. 17, 2019 Cargill, Incorporated's Supplemental Claim Construction Brief Regarding The “Acyl-Coa Substrate” Claim Term, in the United States District Court for the Eastern District of Virginia Norfolk Division, Civil… [cited by applicant]
Apr. 30, 2019 Claim Construction Order and Opinion, in the United States District Court for the Eastern District of Virginia Norfolk Division, Civil Action No. 2:17-cv-00503-HCM-LRL. [cited by applicant]
May 15, 2019 Supplemental Claim Construction Opinion and Order, in the United States District Court for the Eastern District of Virginia Norfolk Division, Civil Action No. 2:17-cv-00503-HCM-LRL. [cited by applicant]
Jun. 19, 2019 Response to the Dec. 19, 2018 Fourth Examination Report filed in connection with Canadian Patent Application No. 2,743,880, Cite ID No. NP1-280. [cited by applicant]
Oct. 4, 2018 Plaintiff BASF Plant Science, LP's Objections and Responses to Commonwealth Scientific and Industrial Research Organisation's First Set Of Interrogatories (Excerpted for Interrogatory No. 5 and 7), in the U… [cited by applicant]
Oct. 31, 2018 Cargill Incorporated's Responses to Counterclaimant Commonwealth Scientific and Industrial Research Organisation's First Set of Interrogatories (Excerpted for Interrogatory Nos. 5 and 7), in the United Sta… [cited by applicant]
Jan. 18, 2019 Plaintiff BASF Plant Science, LP's Supplemental Responses to Commonwealth Scientific and Industrial Research Organisation's Interrogatory No. 5 and 7, in the United States District Court for the Eastern Di… [cited by applicant]
Feb. 25, 2019 Cargill Incorporated's Supplemental Responses to Counterclaimant Commonwealth Scientific and Industrial Research Organisation's First Set of Interrogatories (Excerpted for Interrogatory No. 5 and 7), in th… [cited by applicant]
May 13, 2019 BASF Plant Science, LP's Second Supplemental Response to Commonwealth Scientific and Industrial Research Organisation's Interrogatory No. 5 and 7, in the United States District Court for the Eastern Distric… [cited by applicant]
May 13, 2019 Cargill Incorporated's Second Supplemental Responses to Counterclaimant Commonwealth Scientific and Industrial Research Organisation's First Set of Interrogatories, in the United States District Court for t… [cited by applicant]
May 13, 2019 BASF Plant Science, LP's and BASF Plant Science Gmbh's Objections and Responses to Commonwealth Scientific and Industrial Organisation's Second Set of Interrogatories (Request No. 14-25) (Excerpted), in the… [cited by applicant]
May 29, 2019 Expert Report of Denis J. Murphy, Ph.D. (redacted version), in the United States District Court for the Eastern District of Virginia Norfolk Division, Civil Action No. 2:17-cv-00503-HCM-LRL. [cited by applicant]
Jul. 1, 2019 Rebuttal Validity Expert Report of Ljerka Kunst, Ph.D., in the United States District Court for the Eastern District of Virginia Norfolk Division, Civil Action No. 2:17-cv-00503-HCM-LRL. [cited by applicant]
Aug. 6, 2019 Office Action issued in connection with Japanese Patent Application No. JP 2018-167852 and its English translation. [cited by applicant]
Aug. 6, 2019 Office Action issued in connection with Japanese Patent Application No. JP 2018-167853 and its English translation. [cited by applicant]
Aug. 20, 2019 First Examination Report issued in connection with Australian Patent Application No. 2017276232. [cited by applicant]
Sep. 12, 2019 Response to Jun. 17, 2019 Examination Report issued in connection with Canadian Patent Application No. 3,015,426. [cited by applicant]
Sep. 30, 2019 Notice of Hearing issued in connection with corresponding Indian patent application 3829/CHENP/2011 and its English translation. [cited by applicant]
Nov. 6, 2018 Protective Order Signed by District Judge Henry C. Morgan, Jr. in the United States District Court for the Eastern District of Virginia Norfolk Division, Civil Action No. 2:17-cv-00503-HCM-LRL. [cited by applicant]
Nov. 1, 2019 Jury Verdict Form in United States District Court for the Eastern District of Virginia Norfolk Division, Civil Action No. 2:17-cv-00503-HCM-LRL. [cited by applicant]
Dec. 23, 2019 Judgment in a Civil Case issued in United States District Court for the Eastern District of Virginia Norfolk Division, Civil Action No. 2:17-cv-00503-HCM-LRL. [cited by applicant]
Dec. 23, 2019 Opinion and Order Regarding Remedies issued in United States District Court for the Eastern District of Virginia Norfolk Division, Civil Action No. 2:17-cv-00503-HCM-LRL. [cited by applicant]
Aug. 23, 2019 Counterclaimants' Answer to BASF Plant Science, LP And BASF Plant Science Gmbh's Amended Counterclaims Against Counterclaimants (Public Redacted Version), filed in the United States District Court for the … [cited by applicant]
Sep. 4, 2019 BASF/Cargill's Brief in Support Of Their Daubert Motion to Exclude Portions of the Testimony Of Dr. Ljerka Kunst And Mr. John C. Jarosz (Public Redacted Version), filed in the United States District Court f… [cited by applicant]
Sep. 4, 2019 Counterclaimants' Memorandum in Support of Motion to Exclude Certain Opinions and Preclude Certain Testimony By Defendants' Experts Brian Napper And Dr. Denis Murphy (Public Redacted Version), filed in the … [cited by applicant]
Sep. 4, 2019 Counterclaimants' Memorandum in Support of Motion For Summary Judgment (Public Redacted Version), filed in the United States District Court for the Eastern District of Virginia Norfolk Division, Civil Actio… [cited by applicant]
Sep. 6, 2019 Counterclaimants' Memorandum in Support of Motions in Limine (No. 1-13) (Public Redacted Version), filed in the United States District Court for the Eastern District of Virginia Norfolk Division, Civil Acti… [cited by applicant]
Sep. 6, 2019 BASF's and Cargill's Motions in Limine (Public Redacted Version), filed in the United States District Court for the Eastern District of Virginia Norfolk Division, Civil Action No. 2:17-cv-00503-HCM-LRL. [cited by applicant]
Sep. 13, 2019 Counterclaimants' Opposition to BASF And Cargill's Daubert Motion to Exclude Portions of The Testimony of Dr. Ljerka Kunst and Mr. John Jarosz (Public Redacted Version), filed in the United States District… [cited by applicant]
Sep. 14, 2019 BASF/Cargill's Memorandum in Opposition to Counterclaimants' Motion for Summary Judgment (Public Redacted Version), filed in the United States District Court for the Eastern District of Virginia Norfolk Di… [cited by applicant]
Sep. 14, 2019 BASF/Cargill's Memorandum in Opposition to Counterclaimants' Daubert Motion (Public Redacted Version), filed in the United States District Court for the Eastern District of Virginia Norfolk Division, Civil… [cited by applicant]
Sep. 19, 2019 Counterclaimants' Opposition to BASF's and Cargill's Motions in Limine (Public Redacted Version), filed in the United States District Court for the Eastern District of Virginia Norfolk Division, Civil Acti… [cited by applicant]
Sep. 19, 2019 Counterclaimants' Reply in Support of Motion to Exclude Certain Opinions and Preclude Certain Testimony by Defendants' Experts Brian Napper and Dr. Denis Murphy (Public Redacted Version), filed in the Unit… [cited by applicant]
Sep. 19, 2019 BASF/Cargill's Reply Brief in Support of Their Daubert Motion to Exclude Portions of the Testimony of Dr. Ljerka Kunst and Mr. John C. Jarosz (Public Redacted Version), filed in the United States District … [cited by applicant]
Sep. 19, 2019 Counterclaimants' Reply in Support of Motion for Summary Judgment (Public Redacted Version), filed in the United States District Court for the Eastern District of Virginia Norfolk Division, Civil Action No… [cited by applicant]
Sep. 30, 2019 BASF/Cargill's Memorandum in Opposition to Counterclaimants' Motions in Limine (Public Redacted Version), filed in the United States District Court for the Eastern District of Virginia Norfolk Division, Ci… [cited by applicant]
Sep. 27, 2019 Transcript of Proceedings (Motions Hearing) in the United States District Court for the Eastern District of Virginia Norfolk Division, Civil Action No. 2:17- CV-00503-HCM-LRL. [cited by applicant]
Oct. 3, 2019 Proposed Final Pretrial Order in the United States District Court for the Eastern District of Virginia Norfolk Division, Civil Action No. 2:17-cv-00503-HCM- LRL. [cited by applicant]
Oct. 8, 2019 BASF's Brief Supporting Trial by Jury of BASF's MTEA-Based Claims and Defenses (Public Redacted Version), filed in the United States District Court for the Eastern District of Virginia Norfolk Division, Civ… [cited by applicant]
Oct. 8, 2019 CSIRO, Nuseed, and GRDC's Memorandum in Support of Excluding BASF's MTEA Counterclaims and Related Defenses from Trial (Public Redacted Version), filed in the United States District Court for the Eastern Di… [cited by applicant]
Oct. 28, 2019 Proponents' Motion Seeking Dismissal of Opponents' Unclean Hands Defense (Public Redacted Version), filed in the United States District Court for the Eastern District of Virginia Norfolk Division, Civil Ac… [cited by applicant]
Oct. 28, 2019 BASF/Cargill's Proposed Final Jury Instructions, filed in the United States District Court for the Eastern District of Virginia Norfolk Division, Civil Action No. 2:17-cv-00503-HCM-LRL. [cited by applicant]
Oct. 28, 2019 Counterclaimants' Final Proposed Jury Instructions—Liability Phase, filed in the United States District Court for the Eastern District of Virginia Norfolk Division, Civil Action No. 2:17-cv-00503-HCM-LRL. [cited by applicant]
Oct. 29, 2019 Proponents' Trial Brief in Support of Jury Instructions Regarding Continuation Patent Applications, Corroborating Evidence of Conception, and Willful Infringement, filed in the United States District Court… [cited by applicant]
Oct. 30, 2019 Counterclaimants' Final Proposed Jury Instructions—Liability Phase, filed in the United States District Court for the Eastern District of Virginia Norfolk Division, Civil Action No. 2:17-cv-00503-HCM-LRL. [cited by applicant]
Oct. 30, 2019 Proponents' Memorandum Regarding a Jury Instruction Concerning Written Description, filed in the United States District Court for the Eastern District of Virginia Norfolk Division, Civil Action No. 2:17-cv… [cited by applicant]
Oct. 30, 2019 Proponents' Brief in Support of Motion Under Fed. R. Civ. P. 50(A), filed in the United States District Court for the Eastern District of Virginia Norfolk Division, Civil Action No. 2:17-cv-00503-HCM-LRL. [cited by applicant]
Oct. 31, 2019 Opponents' Brief in Support of Motion Under Fed. R. Civ. P. 50(A) Concerning Co-Ownership Under Mtea, Lack of Written Description, and Obviousness, filed in the United States District Court for the Eastern… [cited by applicant]
Oct. 31, 2019 Proponents' Proposed Jury Instruction on MTEA, filed in the United States District Court for the Eastern District of Virginia Norfolk Division, Civil Action No. 2:17-cv-00503-HCM-LRL. [cited by applicant]
Oct. 31, 2019 Proponents' Supplemental Memorandum in Support of its Motion for Judgment as a Matter of Law to Address Additional Bases for Relief Under Fed. R. Civ. Proc. 50, filed in the United States District Court fo… [cited by applicant]
Dec. 2, 2019 Proponents' Memorandum in Support of Motions for Judgment as a Matter of Law and a New Trial as to Jury Issues Not Decided by Verdict Under Fed. R. Civ. P. 50(B) and 59, filed in the United States District … [cited by applicant]
Dec. 16, 2019 Opponents' Brief in Opposition to Proponents' Motions for Judgment as a Matter of Law and a New Trial as to Jury Issues Not Decided by Verdict Under Fed. R. Civ. P. 50(B) and 59, filed in the United States… [cited by applicant]
Dec. 23, 2019 Proponents' Reply Brief in Support of Their Motion for Judgment as a Matter of Law and Motion for a New Trial as to Jury Issues Not Decided by Verdict, filed in the United States District Court for the Eas… [cited by applicant]
Aug. 15, 2019 Opinion and Order on Motions to Seal issued in the United States District Court for the Eastern District of Virginia Norfolk Division, Civil Action No. 2:17-cv-00503-HCM-LRL. [cited by applicant]
Jan. 11, 2019 BASF Plant Science, LP's Answer and Amended Affirmative Defenses to Counterclaimants' Amended Counterclaims and BASF Plant Science, LP and BASF Plant Science GMBH's Amended Counterclaims Against Countercla… [cited by applicant]
Translation of Office Action which issued in connection with corresponding Argentinian Patent Application No. AR2009010445. [cited by applicant]
Jan. 13, 2020 Written Submission filed in connection with Indian Patent Application No. 3829/CHENP/2011. [cited by applicant]
May 13, 2020 First Office Action and its English translation issued in connection with corresponding Chinese patent Application No. 201710929893. [cited by applicant]
Sep. 29, 2020 Appeal Brief filed in connection with Indian Patent Application No. 3829/CHENP/2011. [cited by applicant]
Nov. 19, 2020 Office Action issued in connection with Brazilian Patent Application No. PI0921467-4 including English translation thereof. [cited by applicant]
Jan. 15, 2021 Third Communication pursuant to Article 94(3) EPC issued in connection with European Patent Application No. 17182502.9 (Divisional of EP 2358882). [cited by applicant]
Jan. 20, 2021 Second Office Action issued in connection with Chinese Patent Application No. 201710929893.1 including English translation thereof. [cited by applicant]
Apr. 23, 2021 Written Opinion issued in connection with Brazilian Patent Application No. PI0921467-4 including English translation thereof. [cited by applicant]
Apr. 23, 2021 Written Opinion issued in connection with Brazilian Patent Application No. BR122021003836-8 including English translation thereof. [cited by applicant]
Apr. 23, 2021 Written Opinion issued in connection with Brazilian Patent Application No. BR122021003835-0 including English translation thereof. [cited by applicant]
Apr. 23, 2021 Written Opinion issued in connection with Brazilian Patent Application No. BR122021003871-6 including English translation thereof. [cited by applicant]
Jul. 7, 2021 First Examination Report issued in connection with Canadian Patent Application No. 3082380. [cited by applicant]
Oct. 26, 2021 Response to Jan. 15, 2021 Examination Report filed in connection with European Patent Application No. 17182502.9 (Divisional of EP 2358882). [cited by applicant]
Nov. 8, 2021 Response to Jul. 7, 2021 First Examination Report filed in connection with Canadian Patent Application No. 3082380. [cited by applicant]
Jan. 21, 2022 First Examination Report issued in connection with Indian Patent Application 201948054509 (Divisional of IN 3829/CHENP/2011). [cited by applicant]