IP Library › Granted Patent US 12,203,104
Granted Patent B2
US 12,203,104 · App. 17/720,027 · Granted Jan 21, 2025

Flavonoid and anthocyanin bioproduction using microorganism hosts

Inventors: Jingyi Li (San Diego, CA); Nicholas Brideau (San Diego, CA); Joshua Britton (San Diego, CA); Erik Holtzapple (San Diego, CA)
Assignee: DEBUT BIOTECHNOLOGY, INC.
C12N9/0073C12N1/20C12N9/0042C12N9/0071C12N9/1029C12N9/1085C12N9/88C12N9/90C12N9/93C12N15/04C12N15/52C12N15/70C12N15/8243C12N15/825C12P7/42C12P17/06C12P19/44C12Y106/02004C12Y114/11009C12Y114/13021
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Quick Facts
Patent No.
US 12,203,104
App. No.
17/720,027
Granted
Jan 21, 2025
Kind
B2
Abstract

The invention is directed to methods involved in the production of flavonoids, anthocyanins and other organic compounds. The invention provides cells engineered for the production of flavonoids, anthocyanins and other organic compounds, where the engineered cells include one or more genetic modifications that increase flavonoid production by increasing metabolic flux to flavonoid precursors and/or reducing carbon losses resulting from the production of byproducts.

Claims (10)

1. An engineered host cell, wherein the engineered host cell comprises one or more genetic modifications to an endogenous gene to increase the production and/or availability of malonyl-CoA in comparison to a wild-type host cell, wherein the engineered host cell expresses acetyl-CoA carboxylase (ACC) having an amino acid sequence at least 85% identical to the polypeptide set forth in SEQ ID NO: 15.

2. The engineered host cell of claim 1 , wherein the production and/or availability of malonyl-CoA is increased by transformation of acetyl-CoA to malonyl-CoA.

3. The engineered host cell of claim 1 , wherein the engineered host cell comprises one or more genetic modifications to increase expression of acetyl-CoA carboxylase (ACC) in comparison to a wild-type host cell.

4. The engineered host cell of claim 1 , wherein the engineered host cell is E. coli.

5. The engineered host cell of claim 4 , wherein the E. coli further comprises genes from fungi.

6. The engineered host cell of claim 3 , wherein the acetyl-CoA carboxylase is from a species selected from the group consisting of Mucor circinelloides, Rhodotorula toruloides, Lipomyces starkeyi, and Ustilago maydis , and orthologs of acetyl-CoA carboxylase having at least 50% amino acid identity to the acetyl-CoA carboxylase of these aforementioned species.

7. The engineered host cell of claim 1 , wherein the one or more genetic modification is deletion or attenuation of one or more fatty acid biosynthetic genes resulting in decrease in fatty acid biosynthesis in comparison to a wild-type host cell.

8. The engineered host cell of claim 1 , wherein the engineered host cell further comprises-peptides selected from a group consisting of: (i) malonate CoA-transferase having an amino acid sequence at least 80% identical to the polypeptide set forth in SEQ ID NO: 19; (ii) acetyl-CoA synthase (ACS) having an amino acid sequence at least 80% identical to the polypeptide set forth in SEQ ID NO: 16; (iii) malonyl-CoA synthase having an amino acid sequence at least 80% identical SEQ ID NO: 77, SEQ ID NO: 78, or SEQ ID NO: 79; (iv) malonate transporter having an amino acid sequence at least 80% identical to SEQ ID NO: 80, SEQ ID NO: 81, SEQ ID NO: 82, SEQ ID NO: 83, SEQ ID NO: 84, SEQ ID NO: 85, SEQ ID NO: 86, or SEQ ID NO: 87; (v) pantothenate kinase having an amino acid sequence at least 80% identical to SEQ ID NO: 88, SEQ ID NO: 89, or SEQ ID NO: 90; and (vi) any combinations thereof.

9. The engineered host cell of claim 1 , wherein the engineered host cell expresses acetyl-CoA carboxylase (ACC) having an amino acid sequence at least 90% identical to the polypeptide set forth in SEQ ID NO: 15.

10. The engineered host cell of claim 1 , wherein the engineered host cell expresses acetyl-CoA carboxylase (ACC) having an amino acid sequence at least 95% identical to the polypeptide set forth in SEQ ID NO: 15.

Assignments (1)
ASSIGNMENT OF ASSIGNOR'S INTEREST Recorded Apr 26, 2022
From: LI, JINGYI; BRIDEAU, NICHOLAS; BRITTON, JOSHUA; HOLTZAPPLE, ERIK
To: DEBUT BIOTECHNOLOGY, INC.
Reel/Frame 059732/0403 →
Continuity (2)
Provisional Application 63174403 · Apr 13, 2021
Related Publication 20220333122A1 · Oct 20, 2022
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