IP Library › Granted Patent US 12,216,033
Granted Patent B2
US 12,216,033 · App. 18/351,796 · Granted Feb 4, 2025

Contact-type patch, staining method using the same, and manufacturing method thereof

Inventors: Dongyoung Lee (Yongin-si, KR); Kyunghwan Kim (Yongin-si, KR); Youngmin Shin (Yongin-si, KR); Hyunjeong Yang (Seongnam-si, KR); Chanyang Lim (Seongnam-si, KR)
Assignee: NOUL CO, LTD.
G01N1/312B01L3/00C07K16/3061C12Q1/6844C12Q1/6848C12Q1/686C12Q1/701G01N1/30G01N1/31G01N15/06G01N15/14G01N21/77G01N33/4833G01N33/49G01N33/5082G01N33/52G01N33/5304G01N33/533G01N33/574G01N33/60G06T7/0012G06T7/0014B01L3/505B01L7/52G01N2001/302G01N15/01G01N15/075G01N2021/7723G01N2021/7786
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Quick Facts
Patent No.
US 12,216,033
App. No.
18/351,796
Granted
Feb 4, 2025
Kind
B2
Abstract

The present disclosure relates to a gel-phase patch that performs a function of assisting in staining during a staining process such as a process of coming into contact with a specimen such as blood to perform a staining function of staining the specimen, a process of fixing the specimen, or a process of forming an optimal pH at a specimen stained by a staining sample. According to an aspect of the present disclosure, a contact-type staining patch includes a staining solution that reacts with a specimen and a gel receptor provided as a gel matrix of a mesh structure in which a pore that accommodates the staining solution is formed and the mesh structure prevents the staining solution in the pore from leaking or degenerating, and having a contact surface that comes into contact with the specimen to transfer some of the staining solution to the specimen.

Claims (11)

1. A method of staining a specimen using a gel-phase contact-type staining patch, the method comprising:

staining a specimen fixed on a slide with a staining reagent,

wherein, the staining step comprises,

contacting a surface of the gel-phase contact-type staining patch comprising a staining reagent configured to react with the specimen and a buffer solution to the specimen fixed on the slide; and

separating the gel-phase contact-type staining patch from the specimen.

2. The method of claim 1 , wherein the buffer solution has a predetermined pH value, wherein the predetermined pH value is identical to an optimal pH value of the staining reagent.

3. The method of claim 1 , wherein the buffer solution has a predetermined pH value, wherein the predetermined pH value is smaller than the optimal pH of the staining reagent when the optimal pH is acidic, and is larger than the optimal pH of the staining reagent when the optimal pH is basic.

4. The method of claim 1 , wherein the gel is any one selected from the group consisting of hydrogel, silicone gel, silica gel, agar gel, agarose gel, PDMS (polydimethylsiloxane) gel, and PMMA (polymethylmethacrylate) gel.

5. The method of claim 1 , wherein the contacting the surface of the gel-phase contact-type staining patch to the specimen fixed on the slide is performed by applying predetermined pressure.

6. The method of claim 1 , wherein the contacting the surface of the gel-phase contact-type staining patch to the specimen fixed on the slide is performed without applying any external pressure.

7. The method of claim 1 , wherein the staining reagent is any one selected from the group consisting of acetocarmine, methylene blue, eosin, Azure II, acidic fuchsin, crystal violet, safranin, Janus green B, hemotoxylin, Giemsa solution, Wright solution, Wright-Giemsa solution, Romanowski staining solution, Leishman staining solution, Gram staining solution, carbol-fuchsin, Ziehl solution, DNA probe coupled to DAPI (4,6-diamidino-2-phenylindole) fluorochorme, a fluorescent substance, an antibody coupled to an enzyme, an antibody coupled to a fluorescent substance, and an antibody coupled to an isotope.

Assignments (1)
ASSIGNMENT OF ASSIGNOR'S INTEREST Recorded Apr 19, 2024
From: LEE, DONGYOUNG; KIM, KYUNGHWAN; SHIN, YOUNGMIN; YANG, HYUNJEONG; LIM, CHANYANG
To: NOUL CO, LTD.
Reel/Frame 067169/0520 →
Priority Claims (3)
KR 10-2016-0069936 · Jun 4, 2016 · national
KR 10-2016-0069937 · Jun 4, 2016 · national
KR 10-2016-0069938 · Jun 4, 2016 · national
Continuity (5)
Continuation 16417419 · May 20, 2019
Division 15498343 · Apr 26, 2017
Continuation 15206247 · Jul 9, 2016
Provisional Application 62298959 · Feb 23, 2016
Related Publication 20230366792A1 · Nov 16, 2023
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