IP Library › Granted Patent US 12,263,270
Granted Patent B2
US 12,263,270 · App. 17/434,925 · Granted Apr 1, 2025

Acoustic extracellular matrix hydrogels and their use

Inventors: Stephen Francis Badylak (West Lafayette, IN); George S. Hussey (Cranberry Township, PA)
Assignee: University of Pittsburgh—Of the Commonwealth System of Higher Education
A61L26/0057A61L26/008A61L27/3633A61L27/52A61L2400/04
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Quick Facts
Patent No.
US 12,263,270
App. No.
17/434,925
Granted
Apr 1, 2025
Kind
B2
Abstract

Methods are disclosed herein for producing a mammalian acoustic extracellular matrix (ECM) hydrogel. In further embodiments, mammalian acoustic ECM hydrogels are disclosed that are produced using the disclosed methods. Also disclosed is a mammalian acoustic ECM hydrogel, wherein the hydrogel is thermoreversible. Methods of using these acoustic ECM hydrogels are also disclosed.

Claims (27)

1. A method of producing an extracellular matrix (ECM) hydrogel, comprising:

solubilizing mammalian ECM in a liquid using ultrasound frequency to produce an acoustic ECM hydrogel in a liquid phase, wherein the mammalian ECM is present at a concentration of about 25 mg/ml to about 600 mg/ml.

2. The method of claim 1 , wherein the ultrasound frequency is applied to the ECM in the liquid at a temperature between 30° C. to 43° C.

3. The method of claim 1 , wherein the ultrasound frequency is about 20 kHz to about 100 kHz.

4. The method of claim 3 , wherein the ultrasound frequency is applied to the ECM in the liquid for at least 30 seconds.

5. A method of producing an extracellular matrix (ECM) hydrogel, comprising:

solubilizing mammalian ECM in a liquid at a concentration of about 25 mg/ml to about 600 mg/ml with ultrasound at a frequency of about 20 kHz to about 100 kHz at for at least about 60 seconds at a temperature of greater than about 37° C. to produce an acoustic ECM hydrogel in a liquid phase.

6. The method of claim 5 , wherein the ultrasound frequency is applied to the mammalian ECM in the liquid for about 1 minute to about 5 minutes.

7. The method of claim 1 , wherein the mammalian ECM is lyophilized mammalian ECM.

8. The method of claim 1 , wherein the mammalian ECM is provided as pieces in the range of about 10 μm to about 2000 μm.

9. The method of claim 5 , further comprising cooling the acoustic ECM hydrogel in the liquid phase to a temperature of about 37° C. or less, thereby producing the acoustic ECM hydrogel in a gel phase.

10. The method of claim 5 , wherein the ultrasound is at a frequency of about 20 kHz.

11. The method of claim 1 , wherein the ultrasound has an amplitude of about 20 μm to about 320 μm.

12. The method of claim 1 , wherein the mammalian ECM is present at a concentration of about 25 mg/ml to about 150 mg/ml.

13. The method of claim 1 , wherein the liquid is phosphate buffered saline.

14. The method of claim 1 , wherein the ECM is a urinary bladder ECM, a small intestinal submucosal ECM, an esophageal ECM, a trachea ECM, a liver ECM or a dermal ECM.

15. The method of claim 14 , wherein the ECM is porcine ECM or bovine ECM.

16. The method of claim 1 , wherein the acoustic ECM hydrogel is gamma irradiated.

17. A method of producing an extracellular matrix (ECM) hydrogel comprising:

solubilizing mammalian ECM in a liquid using ultrasound frequency to produce an acoustic ECM hydrogel, wherein the ultrasound frequency is about 20 kHz to about 100 kHz.

18. The method of claim 17 , wherein the mammalian ECM is present at a concentration of about 25 mg/ml to about 150 mg/ml and is provided as pieces in the range of about 10 μm to about 2000 μm.

19. The method of claim 18 , wherein the ultrasound frequency is applied to the ECM in the liquid at a temperature of 30° C. to 43° C.

20. The method of claim 19 , wherein the ultrasound has an amplitude of about 20 μm to about 320 μm.

21. The method of claim 20 , wherein the ultrasound frequency is applied to the ECM in the liquid for about 30 seconds to about 5 minutes.

22. The method of claim 17 , wherein the ECM hydrogel undergoes a gel to sol transition at about 37° C.

23. The method of claim 17 , wherein the mammalian ECM is urinary bladder ECM, small intestinal submucosal ECM, esophageal ECM, tracheal ECM, liver ECM, or dermal ECM.

24. The method of claim 17 , wherein the ECM is porcine ECM or bovine ECM.

Assignments (1)
ASSIGNMENT OF ASSIGNOR'S INTEREST Recorded Aug 31, 2021
From: BADYLAK, STEPHEN FRANCIS; HUSSEY, GEORGE S.
To: UNIVERSITY OF PITTSBURGH - OF THE COMMONWEALTH SYSTEM OF HIGHER EDUCATION
Reel/Frame 057344/0377 →
Continuity (3)
Provisional Application 62950565 · Dec 19, 2019
Provisional Application 62817787 · Mar 13, 2019
Related Publication 20220143265A1 · May 12, 2022
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