Phase-contrast microscope with ring-structured fiber lighting and a conjugate-positioned annular phase film
View Patent ↗A phase-contrast microscope includes a light source section configured to emit light; a light guide including a plurality of optical fibers, the light guide transmitting the light emitted from the light source section through the plurality of optical fibers; and an object lens including a lens and an annular phase film, the annular phase film being on the side to which light passes through the lens, the object lens being configured to enlarge an image on a sample irradiated with the light transmitted by the light guide. The plurality of optical fibers include a plurality of emission faces arranged to form a ring, and the light guide is disposed in such a manner that the plurality of emission faces are in a conjugate position to the annular phase film.
1. A phase-contrast microscope comprising:
a light source section configured to emit light;
a light guide comprising a plurality of optical fibers, the light guide transmitting the light emitted from the light source section through the plurality of optical fibers; and
an object lens comprising a lens and an annular phase film, the annular phase film being on a side to which light passes through the lens, the object lens being configured to enlarge an image on a sample irradiated with the light transmitted by the light guide,
wherein
the plurality of optical fibers comprise a plurality of emission faces arranged to form a ring, and
the light guide is disposed in such a manner that the plurality of emission faces are in a conjugate position to the annular phase film.
2. The phase-contrast microscope according to claim 1 further comprising a controller configured to cause light to exit through at least one emission face selected among the plurality of emission faces.
3. The phase-contrast microscope according to claim 2 , wherein
the light source section comprises a plurality of light sources configured to emit light to the plurality of optical fibers, and
the controller causes at least one light source selected among the plurality of light sources to emit light through the at least one emission face.
4. The phase-contrast microscope according to claim 2 , wherein
the ring formed by the plurality of emission faces comprises a plurality of arc regions, and
the controller causes light to exit through at least one emission face among the plurality of emission faces, the at least one emission face forming an arc region selected among the plurality of arc regions.
5. The phase-contrast microscope according to claim 2 , wherein
the plurality of emission faces are arranged to form a plurality of concentric rings, and
the controller causes light to exit through at least one emission face among the plurality of emission faces, the at least one emission face forming a ring selected among the plurality of concentric rings.
6. The phase-contrast microscope according to claim 2 , wherein
the plurality of emission faces emit a plurality of light beams with different peak wavelengths, the plurality of emission faces being arranged in a fixed order repeatedly around a circumference of the ring, and
the controller causes an emission face among the plurality of emission faces to emit a light beam of a selected peak wavelength among the plurality of light beams with different peak wavelengths.
7. The phase-contrast microscope according to claim 1 wherein the plurality of optical fibers comprise multi-mode fibers.
8. The phase-contrast microscope according to claim 1 , wherein
the plurality of optical fibers each comprise a core and a clad coating the core, and
the phase-contrast microscope further comprises a clad-mode-light removing section configured to remove clad mode light propagating through the clad.
9. The phase-contrast microscope according to claim 1 , wherein
the plurality of optical fibers each comprise a core and a clad coating the core, and
the clad comprises a material to remove clad mode light propagating through the clad.
10. The phase-contrast microscope according to claim 1 , wherein
the plurality of optical fibers each comprise a core whose diameter does not exceed a value obtained by dividing a width of the phase film by an imaging magnification of the lens.