IP Library › Granted Patent US 12,390,559
Granted Patent B2
US 12,390,559 · App. 18/658,011 · Granted Aug 19, 2025

Hydrogel medium for the storage and preservation of tissue

Inventors: Scott A. Zawko (Austin, TX); Sarah M. Mayes (Austin, TX)
Assignee: ALAFAIR BIOSCIENCES, INC.
A61L27/52A61L27/26A61L27/3633
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Quick Facts
Patent No.
US 12,390,559
App. No.
18/658,011
Granted
Aug 19, 2025
Kind
B2
Abstract

An embodiment includes therapeutic compositions that include a hydrogel medium and a plurality of tissue segments dispersed within the medium. The hydrogel medium preserves and stores the tissue through processing, transport, and storage. The embodiment addresses an identified problem of preserving a tissue, whether such tissue is fresh, cryopreserved, or sterile. The therapeutic compositions may be placed on or within the body to cover and protect wounds, provide a scaffold for reconstruction, repair, or replacement, and reduce surgical complications as a result of inflammation and scar tissue formation. Other embodiments are described herein.

Claims (62)

1. An apparatus comprising:

a hydrogel including: (a) (i) an upper surface having a width and a length, (a) (ii) a lower surface having a width and a length, (a) (iii) sidewalls that respectively couple the widths and lengths of the upper and lower surfaces to each other;

first, second, third, and fourth segments of non-viable extracellular matrix;

wherein (b) (i) a portion of each of the first and second segments emerges from the upper surface of the hydrogel and a portion of each of the third and fourth segments emerges from the lower surface of the hydrogel, (b) (ii) an additional portion of each of the first, second, third, and fourth segments is submerged within the hydrogel and between the upper and lower surfaces of the hydrogel;

wherein no portion of the first or second segments emerges from the lower surface of the hydrogel and no portion of the third or fourth segments emerges from the upper surface of the hydrogel;

wherein the hydrogel includes hyaluronic acid and alginate.

2. The apparatus of claim 1 , wherein:

the upper surface of the hydrogel includes troughs that collectively form a mesh pattern;

the mesh pattern covers a majority of the upper surface of the hydrogel.

3. The apparatus of claim 1 wherein:

the hyaluronic acid is uncrosslinked;

at least a portion of the alginate is crosslinked with calcium;

the alginate is crosslinked around the uncrosslinked hyaluronic acid to retain the uncrosslinked hyaluronic acid within the alginate.

4. The apparatus of claim 3 included in a kit comprising a calcium chelator.

5. The apparatus of claim 1 , wherein:

the alginate is crosslinked around the first and second segments;

the first and second segments are physically constrained within the hydrogel in response to the alginate being crosslinked around the first and second segments.

6. The apparatus of claim 5 comprising carboxymethylcellulose, wherein the carboxymethylcellulose is physically constrained within the hydrogel in response to the alginate being crosslinked around the carboxymethylcellulose.

7. The apparatus of claim 1 , wherein:

a portion of the hydrogel is included between the first and second segments;

the portion does not include the first and second segments and includes no additional segments of non-viable extracellular matrix;

the portion contacts the upper and lower surfaces of the hydrogel.

8. The apparatus of claim 7 , wherein the portion includes a consistent density from the upper surface of the hydrogel to the lower surface of the hydrogel.

9. The apparatus of claim 1 , wherein:

segments of non-viable extracellular matrix are not evenly distributed throughout the hydrogel;

segments of non-viable extracellular matrix, which do not emerge from the upper or lower surfaces of the hydrogel, are absent from a central core area of the hydrogel;

the central core area includes a middle third of the hydrogel measured parallel to a height of the hydrogel;

the central core area includes a middle third of the hydrogel measured parallel to the length of the hydrogel.

10. The apparatus of claim 1 comprising:

fifth and sixth segments of non-viable extracellular matrix;

wherein no portion of the fifth or sixth segments emerges from the lower surface of the hydrogel;

wherein no portion of the fifth or sixth segments emerges from the upper surface of the hydrogel.

11. An apparatus comprising:

a hydrogel including: (a) (i) an upper surface having a width and a length, (a) (ii) a lower surface having a width and a length, (a) (iii) sidewalls that respectively couple the widths and lengths of the upper and lower surfaces to each other;

first, second, third, and fourth segments of non-viable extracellular matrix;

wherein (b) (i) a portion of each of the first and second segments emerges from the upper surface of the hydrogel, (b) (ii) an additional portion of each of the first and second segments is submerged within the hydrogel and between the upper and lower surfaces of the hydrogel;

wherein no portion of the first or second segments emerges from the lower surface of the hydrogel;

wherein no portion of the third or fourth segments emerges from the lower surface of the hydrogel;

wherein no portion of the third or fourth segments emerges from the upper surface of the hydrogel;

wherein the hydrogel includes hyaluronic acid and alginate.

12. The apparatus of claim 11 , wherein:

the upper surface of the hydrogel includes troughs that collectively form a mesh pattern;

the mesh pattern covers a majority of the upper surface of the hydrogel.

13. The apparatus of claim 11 wherein:

the hyaluronic acid is uncrosslinked;

at least a portion of the alginate is crosslinked with calcium;

the alginate is crosslinked around the uncrosslinked hyaluronic acid to retain the uncrosslinked hyaluronic acid within the alginate.

14. The apparatus of claim 13 included in a kit comprising a calcium chelator.

15. The apparatus of claim 11 , wherein:

the alginate is crosslinked around the first and second segments;

the first and second segments are physically constrained within the hydrogel in response to the alginate being crosslinked around the first and second segments.

16. The apparatus of claim 15 comprising carboxymethylcellulose, wherein the carboxymethylcellulose is physically constrained within the hydrogel in response to the alginate being crosslinked around the carboxymethylcellulose.

17. The apparatus of claim 11 , wherein:

a portion of the hydrogel is included between the first and second segments;

the portion does not include the first and second segments and includes no additional segments of non-viable extracellular matrix;

the portion contacts the upper and lower surfaces of the hydrogel.

18. The apparatus of claim 17 , wherein the portion includes a consistent density from the upper surface of the hydrogel to the lower surface of the hydrogel.

19. The apparatus of claim 11 , wherein:

segments of non-viable extracellular matrix are not evenly distributed throughout the hydrogel;

segments of non-viable extracellular matrix, which do not emerge from the upper or lower surfaces of the hydrogel, are absent from a central core area of the hydrogel;

the central core area includes a middle third of the hydrogel measured parallel to a height of the hydrogel;

the central core area includes a middle third of the hydrogel measured parallel to the length of the hydrogel.

Continuity (4)
Continuation 16485256
Provisional Application 62510977 · May 25, 2017
Provisional Application 62468451 · Mar 8, 2017
Related Publication 20240285830A1 · Aug 29, 2024
References Cited (296)
US 4141973A · Balazs · 1979 [cited by applicant]
US 4196070A · Chao et al. · 1980 [cited by applicant]
US 4818542A · DeLuca et al. · 1989 [cited by applicant]
US 4937270A · Hamilton et al. · 1990 [cited by applicant]
US 5017229A · Burns et al. · 1991 [cited by applicant]
US 5266326A · Barry et al. · 1993 [cited by applicant]
US 5395383A · Adams et al. · 1995 [cited by applicant]
US 5415631A · Churinetz et al. · 1995 [cited by applicant]
US 5531716A · Luzio et al. · 1996 [cited by applicant]
US 5531735A · Thompson · 1996 [cited by applicant]
US 5532221A · Huang et al. · 1996 [cited by applicant]
US 5563186A · Thompson · 1996 [cited by applicant]
US 5578314A · Cochrum et al. · 1996 [cited by applicant]
US 5618561A · Della Valle et al. · 1997 [cited by applicant]
US 5622707A · Dorigatti et al. · 1997 [cited by applicant]
US 5688775A · Renn et al. · 1997 [cited by applicant]
US 5714166A · Tomalia et al. · 1998 [cited by applicant]
US 5750585A · Park et al. · 1998 [cited by applicant]
US 5760200A · Miller et al. · 1998 [cited by applicant]
US 5795584A · Totakura et al. · 1998 [cited by applicant]
US 5863551A · Woerly · 1999 [cited by applicant]
US 5866554A · Shalaby et al. · 1999 [cited by applicant]
US 5874100A · Mahoney et al. · 1999 [cited by applicant]
US 5919442A · Yin et al. · 1999 [cited by applicant]
US 5925009A · Mahoney et al. · 1999 [cited by applicant]
US 5939323A · Valentini et al. · 1999 [cited by applicant]
US 5981821A · Barikosky · 1999 [cited by applicant]
US 5981825A · Brekke · 1999 [cited by applicant]
US 5984948A · Hasson · 1999 [cited by applicant]
US 5993661A · Ruckenstein et al. · 1999 [cited by applicant]
US 6007833A · Chudzik et al. · 1999 [cited by applicant]
US 6030958A · Burns et al. · 2000 [cited by applicant]
US 6060534A · Ronan et al. · 2000 [cited by applicant]
US 6083930A · Roufa et al. · 2000 [cited by applicant]
US 6096018A · Luzio et al. · 2000 [cited by applicant]
US 6124273A · Drohan et al. · 2000 [cited by applicant]
US 6133325A · Schwartz et al. · 2000 [cited by applicant]
US 6150581A · Jiang et al. · 2000 [cited by applicant]
US 6156572A · Bellamkonda et al. · 2000 [cited by applicant]
US 6165201A · Sawhney et al. · 2000 [cited by applicant]
US 6174999B1 · Miller et al. · 2001 [cited by applicant]
US 6184266B1 · Ronan et al. · 2001 [cited by applicant]
US 6235726B1 · Burns et al. · 2001 [cited by applicant]
US 6271278B1 · Park et al. · 2001 [cited by applicant]
US 6281341B1 · Mares-Guia et al. · 2001 [cited by applicant]
US 6294202B1 · Burns et al. · 2001 [cited by applicant]
US 6334968B1 · Shapiro et al. · 2002 [cited by applicant]
US 6368356B1 · Zhong et al. · 2002 [cited by applicant]
US 6372244B1 · Antanavich et al. · 2002 [cited by applicant]
US 6387978B2 · Ronan et al. · 2002 [cited by applicant]
US 6410044B1 · Chudzik et al. · 2002 [cited by applicant]
US 6425918B1 · Shapiro et al. · 2002 [cited by applicant]
US 6497902B1 · Ma · 2002 [cited by applicant]
US 6500777B1 · Wiseman et al. · 2002 [cited by applicant]
US 6511650B1 · Eiselt et al. · 2003 [cited by applicant]
US 6521223B1 · Calias et al. · 2003 [cited by applicant]
US 6548081B2 · Sadozai et al. · 2003 [cited by applicant]
US 6566345B2 · Miller et al. · 2003 [cited by applicant]
US 6599526B2 · Dimitrijevich · 2003 [cited by applicant]
US 6600011B2 · McDonnell et al. · 2003 [cited by applicant]
US 6608117B1 · Gvozdic · 2003 [cited by applicant]
US 6610669B1 · Calias et al. · 2003 [cited by applicant]
US 6630167B2 · Zhang · 2003 [cited by applicant]
US 6630457B1 · Aeschlimann et al. · 2003 [cited by applicant]
US 6638917B1 · Li et al. · 2003 [cited by applicant]
US 6642363B1 · Mooney et al. · 2003 [cited by applicant]
US 6653240B2 · Crawford · 2003 [cited by applicant]
US 6653420B2 · Domschke et al. · 2003 [cited by applicant]
US 6656974B1 · Renn et al. · 2003 [cited by applicant]
US 6693089B1 · Li et al. · 2004 [cited by applicant]
US 6703041B2 · Burns et al. · 2004 [cited by applicant]
US 6703444B2 · Zhao et al. · 2004 [cited by applicant]
US 6723709B1 · Pressato et al. · 2004 [cited by applicant]
US 6750262B1 · Hähnle et al. · 2004 [cited by applicant]
US 6767928B1 · Murphy et al. · 2004 [cited by applicant]
US 6793675B2 · Shapiro et al. · 2004 [cited by applicant]
US 6818018B1 · Sawhney · 2004 [cited by applicant]
US 6841153B1 · Chegini et al. · 2005 [cited by applicant]
US 6869938B1 · Schwartz et al. · 2005 [cited by applicant]
US 6897271B1 · Domschke et al. · 2005 [cited by applicant]
US 6913765B2 · Li et al. · 2005 [cited by applicant]
US 6924370B2 · Chudzik et al. · 2005 [cited by applicant]
US 6943154B2 · Miller et al. · 2005 [cited by applicant]
US 6960617B2 · Omidian et al. · 2005 [cited by applicant]
US 6977231B1 · Matsuda · 2005 [cited by applicant]
US 6991652B2 · Burg · 2006 [cited by applicant]
US 7022313B2 · O'Connor et al. · 2006 [cited by applicant]
US 7060287B1 · Hubbard et al. · 2006 [cited by applicant]
US 7083697B2 · Dao et al. · 2006 [cited by applicant]
US 7201917B2 · Malaviya et al. · 2007 [cited by applicant]
US 7235295B2 · Laurencin et al. · 2007 [cited by applicant]
US 7252832B1 · Stone et al. · 2007 [cited by applicant]
US 7265098B2 · Miller et al. · 2007 [cited by applicant]
US 7347850B2 · Sawhney · 2008 [cited by applicant]
US 7347968B2 · Lim et al. · 2008 [cited by applicant]
US 7347988B2 · Hu et al. · 2008 [cited by applicant]
US 7459021B2 · Bukshpan · 2008 [cited by applicant]
US 7504286B2 · Cho · 2009 [cited by applicant]
US 7553903B2 · Riegel et al. · 2009 [cited by applicant]
US 7572894B2 · Jin et al. · 2009 [cited by applicant]
US 7592418B2 · Pathak et al. · 2009 [cited by applicant]
US 7629388B2 · Mikos et al. · 2009 [cited by applicant]
US 7682540B2 · Boyan et al. · 2010 [cited by applicant]
US 7741476B2 · Lebreton · 2010 [cited by applicant]
US 7758654B2 · Hoganson · 2010 [cited by applicant]
US 7833284B2 · Lieberman · 2010 [cited by applicant]
US 7919542B2 · Hudgins et al. · 2011 [cited by applicant]
US 7968110B2 · Hubbard · 2011 [cited by applicant]
US 7988992B2 · Omidian et al. · 2011 [cited by applicant]
US 7989505B2 · Hu et al. · 2011 [cited by applicant]
US 7998204B2 · Stone et al. · 2011 [cited by applicant]
US 7998380B2 · Turng et al. · 2011 [cited by applicant]
US 8012677B2 · Steen · 2011 [cited by applicant]
US 8025901B2 · Kao et al. · 2011 [cited by applicant]
US 8075908B2 · Delaney · 2011 [cited by applicant]
US 8097273B2 · Fukuhira et al. · 2012 [cited by applicant]
US 8110242B2 · Hawkins et al. · 2012 [cited by applicant]
US 8133840B2 · Mika et al. · 2012 [cited by applicant]
US 8277831B2 · Young et al. · 2012 [cited by applicant]
US 8323675B2 · Greenawalt · 2012 [cited by applicant]
US 8324184B2 · Prestwich et al. · 2012 [cited by applicant]
US 8455001B2 · Ito et al. · 2013 [cited by applicant]
US 8460695B2 · Greenawalt · 2013 [cited by applicant]
US 8551136B2 · Lu · 2013 [cited by applicant]
US 8668863B2 · Zawko et al. · 2014 [cited by applicant]
US 8728499B2 · Zawko et al. · 2014 [cited by applicant]
US 8802115B2 · Altschuler et al. · 2014 [cited by applicant]
US 8809301B2 · Athanasiadis et al. · 2014 [cited by applicant]
US 8927521B2 · Jackson · 2015 [cited by applicant]
US 8946194B2 · Mayes et al. · 2015 [cited by applicant]
US 9034357B2 · Stopek · 2015 [cited by applicant]
US 9095558B2 · Mayes et al. · 2015 [cited by applicant]
US 9320827B2 · Zawko et al. · 2016 [cited by applicant]
US 9371402B2 · Chen et al. · 2016 [cited by applicant]
US 9421221B2 · McKay et al. · 2016 [cited by applicant]
US 9521839B2 · Pelle Meddahi et al. · 2016 [cited by applicant]
US 9662424B2 · Schmidt et al. · 2017 [cited by applicant]
US 9770539B2 · Parakka et al. · 2017 [cited by applicant]
US 9896561B2 · Zawko et al. · 2018 [cited by applicant]
US 9987130B2 · Weber · 2018 [cited by applicant]
US 10786595B2 · Zimnitsky et al. · 2020 [cited by applicant]
US 10850011B2 · Schmidt et al. · 2020 [cited by applicant]
US 10940229B2 · Saito et al. · 2021 [cited by applicant]
US 11541075B2 · Gooding et al. · 2023 [cited by applicant]
US 20020131933A1 · Delmotte · 2002 [cited by applicant]
US 20030134132A1 · Winterton et al. · 2003 [cited by applicant]
US 20040091605A1 · Bayer et al. · 2004 [cited by applicant]
US 20040138329A1 · Hubbell et al. · 2004 [cited by applicant]
US 20040241436A1 · Hsieh et al. · 2004 [cited by applicant]
US 20050107868A1 · Nakayama et al. · 2005 [cited by applicant]
US 20050282148A1 · Warren et al. · 2005 [cited by applicant]
US 20060173394A1 · Stroock et al. · 2006 [cited by applicant]
US 20070015136A1 · Sanchez-Schmitz et al. · 2007 [cited by applicant]
US 20070026038A1 · Bayer et al. · 2007 [cited by applicant]
US 20070031498A1 · Zhong et al. · 2007 [cited by applicant]
US 20070051630A1 · Larsson et al. · 2007 [cited by applicant]
US 20070202084A1 · Sadozai et al. · 2007 [cited by applicant]
US 20080069857A1 · Yeo et al. · 2008 [cited by applicant]
US 20080182012A1 · Fisher et al. · 2008 [cited by applicant]
US 20080254091A1 · Lee et al. · 2008 [cited by applicant]
US 20080264793A1 · Vigh et al. · 2008 [cited by applicant]
US 20080292664A1 · Giammona et al. · 2008 [cited by applicant]
US 20090062233A1 · Ji et al. · 2009 [cited by applicant]
US 20090081265A1 · Peppas · 2009 [cited by applicant]
US 20090170973A1 · Mattiasson et al. · 2009 [cited by applicant]
US 20090181074A1 · Makower et al. · 2009 [cited by applicant]
US 20090294049A1 · Udipi et al. · 2009 [cited by applicant]
US 20100055158A1 · Vitaris et al. · 2010 [cited by applicant]
US 20100062232A1 · Schauer et al. · 2010 [cited by applicant]
US 20100114313A1 · Lack et al. · 2010 [cited by applicant]
US 20100121261A1 · Kablik et al. · 2010 [cited by applicant]
US 20100217403A1 · Champion et al. · 2010 [cited by applicant]
US 20100273667A1 · Kotov et al. · 2010 [cited by applicant]
US 20110008442A1 · Zawko et al. · 2011 [cited by applicant]
US 20110029003A1 · Lavigne et al. · 2011 [cited by applicant]
US 20110070204A1 · Elias · 2011 [cited by applicant]
US 20120039959A1 · Tessmar et al. · 2012 [cited by applicant]
US 20120088832A1 · Mayes et al. · 2012 [cited by applicant]
US 20120213708A1 · Anderson et al. · 2012 [cited by applicant]
US 20120239063A1 · Lee · 2012 [cited by applicant]
US 20120244107A1 · Heckroth et al. · 2012 [cited by applicant]
US 20120282302A1 · McCanless et al. · 2012 [cited by applicant]
US 20130034592A1 · Yamamoto et al. · 2013 [cited by applicant]
US 20130052236A1 · Tessmar et al. · 2013 [cited by applicant]
US 20130095143A1 · Font Perez et al. · 2013 [cited by applicant]
US 20130195789A1 · Lu · 2013 [cited by applicant]
US 20130211320A1 · Alkhamesi et al. · 2013 [cited by applicant]
US 20130252921A1 · Lee et al. · 2013 [cited by applicant]
US 20130316007A1 · Ma et al. · 2013 [cited by applicant]
US 20140050705A1 · Lim et al. · 2014 [cited by applicant]
US 20140256831A1 · Ito et al. · 2014 [cited by applicant]
US 20140322351A1 · Gawande et al. · 2014 [cited by applicant]
US 20150010490A1 · Kim et al. · 2015 [cited by applicant]
US 20150010636A1 · Delaney · 2015 [cited by applicant]
US 20150064143A1 · Lee et al. · 2015 [cited by applicant]
US 20150290327A1 · Zenobi-Wong et al. · 2015 [cited by applicant]
US 20150320915A1 · Schmidt et al. · 2015 [cited by applicant]
US 20160243281A1 · Leach · 2016 [cited by applicant]
US 20160249603A1 · Suryan · 2016 [cited by applicant]
US 20160325006A1 · Laukkanen et al. · 2016 [cited by applicant]
US 20180163012A1 · Zawko et al. · 2018 [cited by applicant]
US 20210269638A1 · Kim et al. · 2021 [cited by applicant]
US 20220168334A1 · Dowling · 2022 [cited by applicant]
US 20230095832A1 · Ng · 2023 [cited by applicant]
US 20230101687A1 · Traverso et al. · 2023 [cited by applicant]
CN 108969392A · 2018 [cited by applicant]
CN 109294002B · 2021 [cited by applicant]
CN 113813448B · 2022 [cited by applicant]
CN 116019973A · 2023 [cited by applicant]
CN 114984300B · 2023 [cited by applicant]
EP 1806367A · 1997 [cited by applicant]
EP 1105094A1 · 2001 [cited by applicant]
EP 1321516B1 · 2006 [cited by applicant]
EP 1778144A2 · 2007 [cited by applicant]
IN 202011004849A · 2021 [cited by applicant]
JP H04235124A · 1992 [cited by applicant]
JP H06100468A · 1994 [cited by applicant]
JP 2001212224A · 2001 [cited by applicant]
JP 2003062057A · 2003 [cited by applicant]
JP 3805654B2 · 2006 [cited by applicant]
JP 3796165B2 · 2006 [cited by applicant]
KR 20020027747A · 2002 [cited by applicant]
KR 20020032351A · 2002 [cited by applicant]
KR 20030055102A · 2003 [cited by applicant]
KR 20120086948A · 2012 [cited by applicant]
KR 101495281B1 · 2015 [cited by applicant]
KR 101507301B1 · 2015 [cited by applicant]
KR 101985368B1 · 2019 [cited by applicant]
KR 102078334B1 · 2020 [cited by applicant]
WO 1997039737A1 · 1997 [cited by applicant]
WO 2002064192A1 · 2002 [cited by applicant]
WO 2002092678A1 · 2002 [cited by applicant]
WO 2005020849A2 · 2005 [cited by applicant]
WO 2009108760A2 · 2009 [cited by applicant]
WO 2013174661A1 · 2013 [cited by applicant]
WO 2014093489A2 · 2014 [cited by applicant]
WO 2015186101A1 · 2015 [cited by applicant]
WO 2018056937A1 · 2018 [cited by applicant]
WO 2020051920A1 · 2020 [cited by applicant]
WO 2022025229A1 · 2022 [cited by applicant]
WO 2022038213A1 · 2022 [cited by applicant]
WO 2022265367A1 · 2022 [cited by applicant]
WO 2022265368A1 · 2022 [cited by applicant]
WO 2023019143A1 · 2023 [cited by applicant]
Cho, W.J., S.H. Oh, and J.H. Lee, “Alginate Film as a Novel Post-Surgical Tissue Adhesion Barrier,” Journal of Biomaterials Science-Polymer Edition, 2010, 14 pages total. [cited by applicant]
Chang, J.-J., et al., “Electrospun anti-adhesion barrier made of chitosan alginate for reducing peritoneal adhesions,” Carbohydrate Polymers, 2012, 9 pages total. [cited by applicant]
Hirasaki, Y., et al., “Development of a Novel Antiadhesive Material, Alginate Flakes, Ex Vivo and In Vivo,” Surgery Today, 2011, 8 pages total. [cited by applicant]
Xu, J.B., J.P. Bartley, and R.A. Johnson, “Preparation and characterization of alginate hydrogel membranes crosslinked using a water-soluble carbodiimide,” Journal of Applied Polymer Science, 2003, 7 pages total. [cited by applicant]
Oerther, S., et al., “Hyaluronate-alginate gel as a novel biomaterial: Mechanical properties and formation mechanism,” 1999, Biotechnology Bioeng, pp. 206-215. [cited by applicant]
Oerther, S., et al., “High interaction alginate-hyaluronate associations by hyaluronate deacetylation for the preparation of efficient biomaterials,” 2000, Biopolymers, pp. 273-281. [cited by applicant]
Ceana, H. Nezhat, et al., “Adhesion Prevention and Management,” Prevention & Management of Laparoendoscopic Surgical Complications, 3rd edition, Society of Laparoendoscopic Surgeons, 2011, 11 total pages. [cited by applicant]
Zhang, Y., et al., “Thermosensitive methyl cellulose-based injectable hydrogels for post-operation anti-adhesion,” Jan. 30, 2014, 8 total pages. [cited by applicant]
Wiseman, D.M., et al., “Metaanalysis of the safety and efficacy of an adhesion barrier (Interceed TC7) in laparotomy,” Apr. 1999, 3 total pages. [cited by applicant]
Dania Al-Jaroudi, MD and Togas Tulandi MD et al., “Adhesion prevention in gynecologic surgery,” Aug. 2005, 9 total pages. [cited by applicant]
Ten Broek, R.P., et al., “Efficacy of polyethylene glycol adhesion barrier after gynecological laparoscopic surgery: results of a randomized controlled pilot study,” 2012, 7 pages total. [cited by applicant]
Ten Broek, R.P., et al., “Benefits and harms of adhesion barriers for abdominal surgery: a systematic review and meta-analysis,” The Lancet, Jan. 4, 2014, 12 pages total. [cited by applicant]
Yang, B., et al., “Prevention of abdominal adhesion formation by thermosensitive PECE-hydrogel in a rat uterine horn model,” Jan. 2011, 10 pages total. [cited by applicant]
Mettler, L., et al., “A safety and efficacy study of a resorbable hydrogel for reduction of post-operative adhesions following myomectomy,” May 2008, 8 pages total. [cited by applicant]
Lauder, C.I., et al., “Use of a modified chitosan-dextran gel to prevent peritoneal adhesions in a porcine hemicolectomy model,” Aug. 2012, 7 pages total. [cited by applicant]
Johns, D.A., et al., “Initial feasibility study of a sprayable hydrogel adhesion barrier system in patients undergoing laparoscopic ovarian surgery,” Aug. 2003, 5 pages total. [cited by applicant]
Tjandra, J.J., et al., “A sprayable hydrogel adhesion barrier facilitates closure of defunctioning loop ileostomy: a randomized trial,” Jun. 2008, 5 pages total. [cited by applicant]
Diamond, M.P., “Reduction of adhesions after uterine myomectomy by Seprafilm membrane (HAL-F): a blinded, prospective, randomized, multicenter clinical study; Seprafilm Adhesion Study Group,” Dec. 1996, 7 pages total. [cited by applicant]
Falabella, C.A., et al., “Novel Macromolecular Crosslinking Hydrogel to Reduce Intra-Abdominal Adhesions,” Journal of Surgical Research, Apr. 2010, 7 total pages. [cited by applicant]
Hill-West, J.L., et al., “Efficacy of adhesion barriers. Resorbable hydrogel, oxidized regenerated cellulose and hyaluronic acid,” Mar. 1996, 5 total pages. [cited by applicant]
Becker, J.M., et al., “Prevention of postoperative abdominal adhesions by a sodium hyaluronate-based bioresorbable membrane: a prospective, randomized, double-blind multicenter study,” Oct. 1996, 2 total pages. [cited by applicant]
Li, T.C., et al., “The value of an absorbable adhesion barrier, Interceed, in the prevention of adhesion reformation following microsurgical adhesiolysis,” Apr. 1994, 5 total pages. [cited by applicant]
Luo, Y., et al., “Cross-linked hyaluronic acid hydrogel films: new biomaterials for drug delivery,” Journal of Controlled Release, Oct. 3, 2000, 16 total pages. [cited by applicant]
Xiao Zheng Shu, et al., “In situ crosslinkable hyaluronan hydrogels for tissue engineering,” Biomaterials, Mar.-Apr. 2004, 10 total pages. [cited by applicant]
Vrijland, W.W., et al., “Fewer intraperitoneal adhesions with use of hyaluronic acid-carboxymethylcellulose membrane: a randomized clinical trial,” Feb. 2002, 7 total pages. [cited by applicant]
Bennett, S.L., et al., “Next-Generation HydroGel Films as Tissue Sealants and Adhesion Barriers,” Nov./Dec. 2003, 7 total pages. [cited by applicant]
European Patent Office, Examination Report mailed Jul. 29, 2020 in European patent application No. 19 156 162.0, 9 pages total. [cited by applicant]
F. Shen, et al., “A Study On The Fabrication Of Porous Chitosan/Gelatin Network Scaffold For Tissue Engineering,” Polymer International, 2000, 4 pages total. [cited by applicant]
K. Lindenhayn, et al., “Retention of Hyaluronic Acid in Alginate Beads: Aspects for In Vitro Cartilage Engineering,” Dec. 23, 1997, 7 pages total. [cited by applicant]
“Halobarrier Gel by Anika”, Anika.com, downloaded Sep. 6, 2023, https://anika.com/medical/products/surgical-solutions/#:˜:text=Hyalobarrier%C2%AE%20Gel%20and%20Hyalobarrier,in%20the%20abdomino%2Dpelvic%20area. [cited by applicant]
“SUPRO Adhesion Barrier Gel”, ansermedical.com, downloaded Sep. 6, 2023, https://www.ansermedical.com/products/adhesion-barrier-supro-gel. [cited by applicant]
“An Absorbable Gel for Adhesion Prevention”, fziomed.com, downloaded Sep. 6, 2023, https://www.fziomed.com/products/peritoneal-surgery/. [cited by applicant]
Froelich et al. “Alginate-Based Materials Loaded with Nanoparticles in Wound Healing”, Pharmaceutics, Apr. 223; 15(4): 1142, https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10143535/. [cited by applicant]
Bovone et al. “Engineering Hydrogel Adhesion for Biomedical Applications via Chemical Design of the Junction”, pubs.acs.org., ACS Biomater. Sci. Eng. 2021, 7, 9, 4048-4076, https://pubs.acs.org/doi/10.1021/acsbiomateria… [cited by applicant]
Andersen et al. “In Situ Gelation for Cell Immobilization and Culture in Alginate Foam Scaffolds”, ncbi.nlm.nih.gov., Tissue Eng Part A., Feb. 1, 2014; 20(3-4): 600-610, https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3926… [cited by applicant]
European Patent Office, “Communication pursuant to Article 94(3) EPC” as mailed Dec. 13, 2017 in European Patent Application 11 831 723.9. [cited by applicant]
Specogna, et al., “Dehydration, Dissolution, and Melting of Cyclodextrin Crystals,” J. Phys. Chem. B 2015, 119, pp. 1433-1442. [cited by applicant]
Zhang, et al., “Ultrafine Cellulose Acetate Fibers with Nanoscale Structural Features,” J. Nanosci. Nanotechnol. 2008, vol. 8, No. 9, pp. 4461-4469. [cited by applicant]
Nie, et al., “Effects of Chain Conformation and Entanglement on the Electrospinning of Pure Alginate,” Biomacromolecules 2008, 9, pp. 1362-1365. [cited by applicant]
Tilley, “Crystals and Crystal Structures,” John Wiley & Sons, Ltd, England, 2006, 16 pages. [cited by applicant]
Rowley, et al., “Alginate hydrogels as synthetic extracellular matrix materials,” Biomaterials, Jan. 1998, 9 pages. [cited by applicant]
Bekkers, et al., “Targeted Dendrotemy Reveals Active and Passive Contributions of the Dendritic Tree to Synaptic Integration and Neuronal Output,” PNAS, Jul. 3, 2007, vol. 104, No. 27, pp. 11447-11452. [cited by applicant]
Ganesh, et al, “Enzymatically Cross-linked Alginic-Hyaluronic acid Composite Hydrogels As Cell Delivery Vehicles,” International Journal of Biological Macromolecules, Apr. 2013, 11 pages. [cited by applicant]
Dahlmann, J., et al, “Fully defined in situ cross-linkable alginate and hyaluronic acid hydrogels for myocardial tissue engineering,” Biomaterials (Jan. 2013) 34(4):940-951. [cited by applicant]
Mohand-Kaci, et al., “Optimized Hyaluronic Acid Design and Culture Conditions for Preservation of Mesenchymal Stem Cell Properties,” Tissue Engineering Part C Methods, Apr. 2013, 13 pages. [cited by applicant]
International Searching Authority, “Notification of Transmittal of the International Search Report and the Written Opinion of the International Searching Authority,” mailed Sep. 10, 2019, in International application No… [cited by applicant]
Badylak, “The extracellular matrix as a biologic scaffold material,” Dec. 14, 2006, 7 pages. [cited by applicant]
Rogers, “Kiln Dried May Not Mean What You Think It Means”, Gibson MeIlvain Company, 2022. [cited by applicant]
Kett Marketing, “Woodworking and the Importance of Moisture Content”, Kett Science of Sensing, 2018. [cited by applicant]
Hsiao et al., “Astrocytes alignment and reactivity on collagen hydrogels patterned with ECM proteins”. Biomaterials, vol. 39, pp. 124-130, Jan. 2015. [cited by applicant]
Poellmann et al., “Geometric microenvironment directs cell morphology on topographically patterned hydrogel substrates”, Acta Biomaterialia, vol. 6, Issue 9, Sep. 2010, pp. 3514-3523. [cited by applicant]
Gulrez et al., “Hydrogels: methods of preparation, characterisation and applications”, Progress in Molecular and Environmental Bioengineering—From Analysis and Modeling to Technology Applications, ISBN: 9789833072685, 2… [cited by applicant]