Granted Patent B2

US 12,411,064 · App. 17/466,389 · Granted Sep 9, 2025

Inventors: Wenjing Li (Shrewsbury, MA); Beatrice Muriithi (Attleboro, MA); Anna Boardman (Watertown, MA); Matthew A. Lauber (North Smithfield, RI)

Assignee: Waters Technologies Corporation

G01N1/4044C12M21/18G01N1/2813G01N1/312G01N2001/282

Abstract

Claims (14)

1. A kit for protein sample preparation, the kit comprising:

a solid support surface with a polymer coating covering the solid support surface, wherein the polymer coating comprises a first coating portion with functionality for bioconjugation and a second coating portion comprising a diol obtained from hydrolysis of an epoxide containing silane selected from the group consisting of a 3-glycidopropyl trifunctional silane and a 2-(3,4-epoxycyclohexyl) ethyl trifunctional silane, wherein the second coating portion renders the surface hydrophilic and reduces non-specific binding between a protein sample and the solid support surface, wherein the polymer coating is present at a total surface coverage of at least 5 μmoles/m 2 ;

a container containing a buffer solution; and

a vessel for containing the solid support surface and the buffer solution, wherein the buffer solution comprises:

5% to 10% acetonitrile;

from about 10 mM to about 20 mM arginine;

from about 100 mM to about 300 mM methionine;

from about 50 mM to about 200 mM Tris-HCl;

from about 10 mM to about 20 mM CaCl 2 ); and

a polyol selected from the group consisting of xylitol, erythritol, glycerol, propylene glycol, and butanediol.

2. The kit of claim 1 , wherein the polyol is xylitol and the concentration of xylitol ranges from about 600 mM to about 680 mM.

3. The kit of claim 1 , wherein the arginine concentration is about 10 mM.

4. The kit of claim 1 , wherein the methionine concentration is about 200 mM.

5. The kit of claim 1 , further comprising a heat source.

Assignments (1)

Continuity (3)

References Cited (77)