Granted Patent
B2
US 12,448,643 · App. 18/212,868 · Granted Oct 21, 2025
Sequencing of nucleic acids via barcoding in discrete entities
Inventors:
Adam R. Abate (Daly City, CA); John R. Haliburton (San Francisco, CA); Freeman Lan (San Francisco, CA); Adam R. Sciambi (San Francisco, CA)
C12Q1/6804B01F25/4338B01F33/3021B01L3/502784C12N15/1096C12Q1/68C12Q1/6806C12Q1/6874B01F21/00B01L3/502707B01L3/502715B01L3/502761B01L7/52B01L2200/0647B01L2200/0652B01L2300/021B01L2300/0645B01L2300/0864B01L2300/0867B01L2300/123B01L2400/0409B01L2400/0415B01L2400/0424B01L2400/043
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Abstract
Microfluidic methods for barcoding nucleic acid target molecules to be analyzed, e.g., via nucleic acid sequencing techniques, are provided. Also provided are microfluidic, droplet-based methods of preparing nucleic acid barcodes for use in various barcoding applications. The methods described herein facilitate high-throughput sequencing of nucleic acid target molecules as well as single cell and single virus genomic, transcriptomic, and/or proteomic analysis/profiling. Systems and devices for practicing the subject methods are also provided.
Claims (29)
1. A method comprising:
encapsulating, within a discrete entity,
a nucleic acid released from a single cell or a product of the released nucleic acid;
an oligonucleotide including a first nucleic acid barcode serving as an indicator of an affinity reagent that was bound to a molecular target of the single cell;
a plurality of primer sequences;
a plurality of copies of a sequence comprising both a second nucleic acid barcode and a universal sequence,
wherein the second nucleic acid barcode serves as an indicator of the discrete entity in which the oligonucleotide including the first nucleic acid barcode and the released nucleic acid or the product of the released nucleic acid are encapsulated;
incorporating a first copy of the sequence comprising the second nucleic acid barcode and the universal sequence with the oligonucleotide including the first nucleic acid barcode by performing nucleic acid amplification using the universal sequence of the first copy and at least a first primer sequence;
incorporating a second copy of the sequence comprising the second nucleic acid barcode and the universal sequence with the released nucleic acid or the product of the released nucleic acid by performing nucleic acid amplification using the universal sequence of the second copy and at least a second primer sequence; and
sequencing the first nucleic acid barcode, the first copy of the sequence comprising the second nucleic acid barcode, and the second copy of the sequence comprising the second nucleic acid barcode.
2. The method of claim 1 , wherein the affinity reagent comprises a peptide.
3. The method of claim 1 , wherein the affinity reagent comprises an antibody or an antigen binding antibody fragment.
4. The method of claim 1 , wherein the affinity reagent does not comprise an antibody.
5. The method of claim 1 , wherein the affinity reagent comprises a drug.
6. The method of claim 1 , wherein the molecular target is a component of a cell.
7. The method of claim 1 , wherein the product of the released nucleic acid is generated by performing reverse transcription on the released nucleic acid.
8. The method of claim 7 , wherein the reverse transcription occurs without nucleic acid amplification.
9. The method of claim 7 , wherein the product of the released nucleic acid is further generated by performing nucleic acid amplification.
10. The method of claim 9 , further comprising sequencing the amplification product.
11. The method of claim 1 , further comprising amplifying the oligonucleotide before incorporating the second nucleic acid barcode.
12. The method of claim 1 , wherein the oligonucleotide including the first nucleic acid barcode further comprises a unique molecular identifier that uniquely identifies the affinity reagent from a plurality of affinity reagents.
13. The method of claim 12 , further comprising: sequencing the unique molecular identifier; and correcting for amplification bias using the sequenced unique molecular identifier.
14. The method of claim 1 , wherein the universal sequence is complementary to a portion of the first primer sequence and a portion of the second primer sequence,
wherein performing nucleic acid amplification using the universal sequence of the first copy and at least the first primer sequence comprises hybridizing the universal sequence of the first copy to the portion of the first primer sequence, and
wherein performing nucleic acid amplification using the universal sequence of the second copy and at least the second primer sequence comprises hybridizing the universal sequence of the second copy to the portion of the second primer sequence.
15. The method of claim 1 , further comprising:
prior to encapsulating the nucleic acid released from the single cell or the product of the released nucleic acid,
encapsulating the single cell with an affinity reagent bound molecular target comprising the oligonucleotide including the first nucleic acid barcode in the discrete entity;
lysing the single cell within the discrete entity such that the discrete entity comprises a released nucleic acid from within the single cell and the oligonucleotide including the first nucleic acid barcode.
Assignments (1)
ASSIGNMENT OF ASSIGNOR'S INTEREST
Recorded Aug 21, 2023
From: ABATE, ADAM R.; HALIBURTON, JOHN; LAN, FREEMAN; SCIAMBI, ADAM R.
To: THE REGENTS OF THE UNIVERSITY OF CALIFORNIA
Reel/Frame 064651/0339 →
Continuity (4)
Provisional Application
62112075
· Feb 4, 2015
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